Deranged Bioenergetics and Defective Redox Capacity in T Lymphocytes and Neutrophils Are Related to Cellular Dysfunction and Increased Oxidative Stress in Patients with Active Systemic Lupus Erythematosus
Urinary excretion of N-benzoyl-glycyl-Nε-(hexanonyl)lysine, a biomarker of oxidative stress, was higher in 26 patients with active systemic lupus erythematosus (SLE) than in 11 non-SLE patients with connective tissue diseases and in 14 healthy volunteers. We hypothesized that increased oxidative str...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/548516 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832565498968014848 |
|---|---|
| author | Ko-Jen Li Cheng-Han Wu Song-Chou Hsieh Ming-Chi Lu Chang-Youh Tsai Chia-Li Yu |
| author_facet | Ko-Jen Li Cheng-Han Wu Song-Chou Hsieh Ming-Chi Lu Chang-Youh Tsai Chia-Li Yu |
| author_sort | Ko-Jen Li |
| collection | DOAJ |
| description | Urinary excretion of N-benzoyl-glycyl-Nε-(hexanonyl)lysine, a biomarker of oxidative stress, was higher in 26 patients with active systemic lupus erythematosus (SLE) than in 11 non-SLE patients with connective tissue diseases and in 14 healthy volunteers. We hypothesized that increased oxidative stress in active SLE might be attributable to deranged bioenergetics, defective reduction-oxidation (redox) capacity, or other factors. We demonstrated that, compared to normal cells, T lymphocytes (T) and polymorphonuclear neutrophils (PMN) of active SLE showed defective expression of facilitative glucose transporters GLUT-3 and GLUT-6, which led to increased intracellular basal lactate and decreased ATP production. In addition, the redox capacity, including intracellular GSH levels and the enzyme activity of glutathione peroxidase (GSH-Px) and γ-glutamyl-transpeptidase (GGT), was decreased in SLE-T. Compared to normal cells, SLE-PMN showed decreased intracellular GSH levels, and GGT enzyme activity was found in SLE-PMN and enhanced expression of CD53, a coprecipitating molecule for GGT. We conclude that deranged cellular bioenergetics and defective redox capacity in T and PMN are responsible for cellular immune dysfunction and are related to increased oxidative stress in active SLE patients. |
| format | Article |
| id | doaj-art-3776c87ac6a84ee1b8064db89a317dcc |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-3776c87ac6a84ee1b8064db89a317dcc2025-02-03T01:07:27ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/548516548516Deranged Bioenergetics and Defective Redox Capacity in T Lymphocytes and Neutrophils Are Related to Cellular Dysfunction and Increased Oxidative Stress in Patients with Active Systemic Lupus ErythematosusKo-Jen Li0Cheng-Han Wu1Song-Chou Hsieh2Ming-Chi Lu3Chang-Youh Tsai4Chia-Li Yu5Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, 7 Chung-Shan South Road, Taipei 100, TaiwanDepartment of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, 7 Chung-Shan South Road, Taipei 100, TaiwanDepartment of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, 7 Chung-Shan South Road, Taipei 100, TaiwanDivision of Immunology, Rheumatology, and Allergy, Buddhist Dalin Tzu-Chi General Hospital, 2 Ming-Shen Road, Chia-Yi 622, TaiwanDivision of Allergy, Immunology, and Rheumatology, Taipei Veterans General Hospital, 201 Shih-Pai Road, Taipei 112, TaiwanDepartment of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, 7 Chung-Shan South Road, Taipei 100, TaiwanUrinary excretion of N-benzoyl-glycyl-Nε-(hexanonyl)lysine, a biomarker of oxidative stress, was higher in 26 patients with active systemic lupus erythematosus (SLE) than in 11 non-SLE patients with connective tissue diseases and in 14 healthy volunteers. We hypothesized that increased oxidative stress in active SLE might be attributable to deranged bioenergetics, defective reduction-oxidation (redox) capacity, or other factors. We demonstrated that, compared to normal cells, T lymphocytes (T) and polymorphonuclear neutrophils (PMN) of active SLE showed defective expression of facilitative glucose transporters GLUT-3 and GLUT-6, which led to increased intracellular basal lactate and decreased ATP production. In addition, the redox capacity, including intracellular GSH levels and the enzyme activity of glutathione peroxidase (GSH-Px) and γ-glutamyl-transpeptidase (GGT), was decreased in SLE-T. Compared to normal cells, SLE-PMN showed decreased intracellular GSH levels, and GGT enzyme activity was found in SLE-PMN and enhanced expression of CD53, a coprecipitating molecule for GGT. We conclude that deranged cellular bioenergetics and defective redox capacity in T and PMN are responsible for cellular immune dysfunction and are related to increased oxidative stress in active SLE patients.http://dx.doi.org/10.1155/2012/548516 |
| spellingShingle | Ko-Jen Li Cheng-Han Wu Song-Chou Hsieh Ming-Chi Lu Chang-Youh Tsai Chia-Li Yu Deranged Bioenergetics and Defective Redox Capacity in T Lymphocytes and Neutrophils Are Related to Cellular Dysfunction and Increased Oxidative Stress in Patients with Active Systemic Lupus Erythematosus Clinical and Developmental Immunology |
| title | Deranged Bioenergetics and Defective Redox Capacity in T Lymphocytes and Neutrophils Are Related to Cellular Dysfunction and Increased Oxidative Stress in Patients with Active Systemic Lupus Erythematosus |
| title_full | Deranged Bioenergetics and Defective Redox Capacity in T Lymphocytes and Neutrophils Are Related to Cellular Dysfunction and Increased Oxidative Stress in Patients with Active Systemic Lupus Erythematosus |
| title_fullStr | Deranged Bioenergetics and Defective Redox Capacity in T Lymphocytes and Neutrophils Are Related to Cellular Dysfunction and Increased Oxidative Stress in Patients with Active Systemic Lupus Erythematosus |
| title_full_unstemmed | Deranged Bioenergetics and Defective Redox Capacity in T Lymphocytes and Neutrophils Are Related to Cellular Dysfunction and Increased Oxidative Stress in Patients with Active Systemic Lupus Erythematosus |
| title_short | Deranged Bioenergetics and Defective Redox Capacity in T Lymphocytes and Neutrophils Are Related to Cellular Dysfunction and Increased Oxidative Stress in Patients with Active Systemic Lupus Erythematosus |
| title_sort | deranged bioenergetics and defective redox capacity in t lymphocytes and neutrophils are related to cellular dysfunction and increased oxidative stress in patients with active systemic lupus erythematosus |
| url | http://dx.doi.org/10.1155/2012/548516 |
| work_keys_str_mv | AT kojenli derangedbioenergeticsanddefectiveredoxcapacityintlymphocytesandneutrophilsarerelatedtocellulardysfunctionandincreasedoxidativestressinpatientswithactivesystemiclupuserythematosus AT chenghanwu derangedbioenergeticsanddefectiveredoxcapacityintlymphocytesandneutrophilsarerelatedtocellulardysfunctionandincreasedoxidativestressinpatientswithactivesystemiclupuserythematosus AT songchouhsieh derangedbioenergeticsanddefectiveredoxcapacityintlymphocytesandneutrophilsarerelatedtocellulardysfunctionandincreasedoxidativestressinpatientswithactivesystemiclupuserythematosus AT mingchilu derangedbioenergeticsanddefectiveredoxcapacityintlymphocytesandneutrophilsarerelatedtocellulardysfunctionandincreasedoxidativestressinpatientswithactivesystemiclupuserythematosus AT changyouhtsai derangedbioenergeticsanddefectiveredoxcapacityintlymphocytesandneutrophilsarerelatedtocellulardysfunctionandincreasedoxidativestressinpatientswithactivesystemiclupuserythematosus AT chialiyu derangedbioenergeticsanddefectiveredoxcapacityintlymphocytesandneutrophilsarerelatedtocellulardysfunctionandincreasedoxidativestressinpatientswithactivesystemiclupuserythematosus |