Findings and methodologies in oral phageome research: a systematic review

Background The oral microbiome serves as both an indicator and a mediator of oral health. Evidence indicates that bacteriophages (phages) are widely present in the oral microbiome and exhibit diverse classifications and interactions with human cells and other microbes. These phages constitute the or...

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Main Authors: Xin Chen, Ting Zou, Guicong Ding, Shan Jiang
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Journal of Oral Microbiology
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Online Access:https://www.tandfonline.com/doi/10.1080/20002297.2024.2417099
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author Xin Chen
Ting Zou
Guicong Ding
Shan Jiang
author_facet Xin Chen
Ting Zou
Guicong Ding
Shan Jiang
author_sort Xin Chen
collection DOAJ
description Background The oral microbiome serves as both an indicator and a mediator of oral health. Evidence indicates that bacteriophages (phages) are widely present in the oral microbiome and exhibit diverse classifications and interactions with human cells and other microbes. These phages constitute the oral phageome, which potentially exerts significant yet unexplored effects on the interplay between oral and general health.Methods Three databases (PubMed/MEDLINE, Embase and Scopus) were searched for metagenomic analyses that investigated the oral phageome. Eligible studies were synthesized based on their methodological approaches and findings.Results A total of 14 articles were included in this systematic review. Among the 14 articles included, there were six studies that discussed disease-related alterations, along with a discursive examination of additional variables such as sampling niches, external interventions and methodologies. The phages that infect Streptococcus Actinomyces Haemophilus, and Veillonella have been discovered to be associated with chronic periodontitis, caries, and pancreatic ductal carcinoma.Conclusions This systematic review focuses on findings and methodologies in oral phageome studies, which were conducted using highly heterogeneous methodologies that explored the oral phageome in multiple directions while placing constraints on quantitative statistics. Combining different kinds of sample types, utilizing the characteristics of different methods, involving both DNA and RNA phages, and differentiating lysogenic and lytic phages should be the distinction of further studies.
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spelling doaj-art-375fbe6c39ee414bb7eaaba6625cbcca2025-08-20T02:48:12ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972024-12-0116110.1080/20002297.2024.2417099Findings and methodologies in oral phageome research: a systematic reviewXin Chen0Ting Zou1Guicong Ding2Shan Jiang3Shenzhen Children’s Hospital, China Medical University (CMU), Shenzhen, ChinaShenzhen Clinical College of Stomatology, School of Stomatology, Southern Medical University, Shenzhen, Guangdong, ChinaShenzhen Children’s Hospital, China Medical University (CMU), Shenzhen, ChinaShenzhen Clinical College of Stomatology, School of Stomatology, Southern Medical University, Shenzhen, Guangdong, ChinaBackground The oral microbiome serves as both an indicator and a mediator of oral health. Evidence indicates that bacteriophages (phages) are widely present in the oral microbiome and exhibit diverse classifications and interactions with human cells and other microbes. These phages constitute the oral phageome, which potentially exerts significant yet unexplored effects on the interplay between oral and general health.Methods Three databases (PubMed/MEDLINE, Embase and Scopus) were searched for metagenomic analyses that investigated the oral phageome. Eligible studies were synthesized based on their methodological approaches and findings.Results A total of 14 articles were included in this systematic review. Among the 14 articles included, there were six studies that discussed disease-related alterations, along with a discursive examination of additional variables such as sampling niches, external interventions and methodologies. The phages that infect Streptococcus Actinomyces Haemophilus, and Veillonella have been discovered to be associated with chronic periodontitis, caries, and pancreatic ductal carcinoma.Conclusions This systematic review focuses on findings and methodologies in oral phageome studies, which were conducted using highly heterogeneous methodologies that explored the oral phageome in multiple directions while placing constraints on quantitative statistics. Combining different kinds of sample types, utilizing the characteristics of different methods, involving both DNA and RNA phages, and differentiating lysogenic and lytic phages should be the distinction of further studies.https://www.tandfonline.com/doi/10.1080/20002297.2024.2417099Oralphageomemethodologymetagenomicsbioinformatics
spellingShingle Xin Chen
Ting Zou
Guicong Ding
Shan Jiang
Findings and methodologies in oral phageome research: a systematic review
Journal of Oral Microbiology
Oral
phageome
methodology
metagenomics
bioinformatics
title Findings and methodologies in oral phageome research: a systematic review
title_full Findings and methodologies in oral phageome research: a systematic review
title_fullStr Findings and methodologies in oral phageome research: a systematic review
title_full_unstemmed Findings and methodologies in oral phageome research: a systematic review
title_short Findings and methodologies in oral phageome research: a systematic review
title_sort findings and methodologies in oral phageome research a systematic review
topic Oral
phageome
methodology
metagenomics
bioinformatics
url https://www.tandfonline.com/doi/10.1080/20002297.2024.2417099
work_keys_str_mv AT xinchen findingsandmethodologiesinoralphageomeresearchasystematicreview
AT tingzou findingsandmethodologiesinoralphageomeresearchasystematicreview
AT guicongding findingsandmethodologiesinoralphageomeresearchasystematicreview
AT shanjiang findingsandmethodologiesinoralphageomeresearchasystematicreview