Fermentation of faba bean flour as a pre-treatment in high-moisture extrusion for simultaneous fortification of vitamin B12 and reduction of raffinose oligosaccharides in meat analogues

Faba bean is a promising ingredient for the production of meat analogues using high-moisture extrusion; however, the presence of antinutrients poses a challenge to its use. Moreover, the deficiency of vitamin B12 in plant-based meat analogues underscores the need to enhance their nutritional profile...

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Main Authors: Katja Kantanen, Bhawani Chamlagain, Valentina Succi, Siri Seppänen, Minnamari Edelmann, Aino Siitonen, Lotta Kemppinen, Asmo Kemppinen, Mikko Kangas, Pekka Varmanen, Vieno Piironen, Kirsi Jouppila
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Future Foods
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666833524002211
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author Katja Kantanen
Bhawani Chamlagain
Valentina Succi
Siri Seppänen
Minnamari Edelmann
Aino Siitonen
Lotta Kemppinen
Asmo Kemppinen
Mikko Kangas
Pekka Varmanen
Vieno Piironen
Kirsi Jouppila
author_facet Katja Kantanen
Bhawani Chamlagain
Valentina Succi
Siri Seppänen
Minnamari Edelmann
Aino Siitonen
Lotta Kemppinen
Asmo Kemppinen
Mikko Kangas
Pekka Varmanen
Vieno Piironen
Kirsi Jouppila
author_sort Katja Kantanen
collection DOAJ
description Faba bean is a promising ingredient for the production of meat analogues using high-moisture extrusion; however, the presence of antinutrients poses a challenge to its use. Moreover, the deficiency of vitamin B12 in plant-based meat analogues underscores the need to enhance their nutritional profile. This study aimed to fortify faba bean flour with vitamin B12 through fermentation with Propionibacterium freudenreichii and Levilactobacillus brevis while concurrently reducing the content of raffinose family oligosaccharides. The fermentation conditions were initially optimised to maximise active vitamin B12 production, ensure adequate raffinose family oligosaccharide degradation, and maintain microbiological safety. The fermentation was then scaled up to 10 kg and 25 kg. The fermented mass was incorporated into high-moisture extrusion using a wet feeding system, along with a mixture of pea protein isolate and oat fibre concentrate to aid texturization. The extrudates containing the fermented mass exhibited a vitamin B12 content of 75–85 ng/g dry matter, with raffinose, stachyose, and verbascose contents reduced by 53, 56, and 86 %, respectively. Extrudates containing the fermented mass had a layered structure and, compared to control extrudates, a softer texture in general, a more brittle structure, lighter colour, and reduced water absorption capacity.
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spelling doaj-art-375654ed9a6741b28e5a729d9df5ea152025-08-20T02:48:58ZengElsevierFuture Foods2666-83352024-12-011010051810.1016/j.fufo.2024.100518Fermentation of faba bean flour as a pre-treatment in high-moisture extrusion for simultaneous fortification of vitamin B12 and reduction of raffinose oligosaccharides in meat analoguesKatja Kantanen0Bhawani Chamlagain1Valentina Succi2Siri Seppänen3Minnamari Edelmann4Aino Siitonen5Lotta Kemppinen6Asmo Kemppinen7Mikko Kangas8Pekka Varmanen9Vieno Piironen10Kirsi Jouppila11Corresponding authors.; Department of Food and Nutrition, University of Helsinki, P.O. Box 66, FI-00014 University of Helsinki, FinlandCorresponding authors.; Department of Food and Nutrition, University of Helsinki, P.O. Box 66, FI-00014 University of Helsinki, FinlandDepartment of Food and Nutrition, University of Helsinki, P.O. Box 66, FI-00014 University of Helsinki, FinlandDepartment of Food and Nutrition, University of Helsinki, P.O. Box 66, FI-00014 University of Helsinki, FinlandDepartment of Food and Nutrition, University of Helsinki, P.O. Box 66, FI-00014 University of Helsinki, FinlandDepartment of Food and Nutrition, University of Helsinki, P.O. Box 66, FI-00014 University of Helsinki, FinlandDepartment of Food and Nutrition, University of Helsinki, P.O. Box 66, FI-00014 University of Helsinki, FinlandDepartment of Food and Nutrition, University of Helsinki, P.O. Box 66, FI-00014 University of Helsinki, FinlandDepartment of Food and Nutrition, University of Helsinki, P.O. Box 66, FI-00014 University of Helsinki, FinlandDepartment of Food and Nutrition, University of Helsinki, P.O. Box 66, FI-00014 University of Helsinki, FinlandDepartment of Food and Nutrition, University of Helsinki, P.O. Box 66, FI-00014 University of Helsinki, FinlandDepartment of Food and Nutrition, University of Helsinki, P.O. Box 66, FI-00014 University of Helsinki, FinlandFaba bean is a promising ingredient for the production of meat analogues using high-moisture extrusion; however, the presence of antinutrients poses a challenge to its use. Moreover, the deficiency of vitamin B12 in plant-based meat analogues underscores the need to enhance their nutritional profile. This study aimed to fortify faba bean flour with vitamin B12 through fermentation with Propionibacterium freudenreichii and Levilactobacillus brevis while concurrently reducing the content of raffinose family oligosaccharides. The fermentation conditions were initially optimised to maximise active vitamin B12 production, ensure adequate raffinose family oligosaccharide degradation, and maintain microbiological safety. The fermentation was then scaled up to 10 kg and 25 kg. The fermented mass was incorporated into high-moisture extrusion using a wet feeding system, along with a mixture of pea protein isolate and oat fibre concentrate to aid texturization. The extrudates containing the fermented mass exhibited a vitamin B12 content of 75–85 ng/g dry matter, with raffinose, stachyose, and verbascose contents reduced by 53, 56, and 86 %, respectively. Extrudates containing the fermented mass had a layered structure and, compared to control extrudates, a softer texture in general, a more brittle structure, lighter colour, and reduced water absorption capacity.http://www.sciencedirect.com/science/article/pii/S2666833524002211FermentationVitamin B12Raffinose family oligosaccharidesHigh-moisture extrusionWet mass feedingFaba bean
spellingShingle Katja Kantanen
Bhawani Chamlagain
Valentina Succi
Siri Seppänen
Minnamari Edelmann
Aino Siitonen
Lotta Kemppinen
Asmo Kemppinen
Mikko Kangas
Pekka Varmanen
Vieno Piironen
Kirsi Jouppila
Fermentation of faba bean flour as a pre-treatment in high-moisture extrusion for simultaneous fortification of vitamin B12 and reduction of raffinose oligosaccharides in meat analogues
Future Foods
Fermentation
Vitamin B12
Raffinose family oligosaccharides
High-moisture extrusion
Wet mass feeding
Faba bean
title Fermentation of faba bean flour as a pre-treatment in high-moisture extrusion for simultaneous fortification of vitamin B12 and reduction of raffinose oligosaccharides in meat analogues
title_full Fermentation of faba bean flour as a pre-treatment in high-moisture extrusion for simultaneous fortification of vitamin B12 and reduction of raffinose oligosaccharides in meat analogues
title_fullStr Fermentation of faba bean flour as a pre-treatment in high-moisture extrusion for simultaneous fortification of vitamin B12 and reduction of raffinose oligosaccharides in meat analogues
title_full_unstemmed Fermentation of faba bean flour as a pre-treatment in high-moisture extrusion for simultaneous fortification of vitamin B12 and reduction of raffinose oligosaccharides in meat analogues
title_short Fermentation of faba bean flour as a pre-treatment in high-moisture extrusion for simultaneous fortification of vitamin B12 and reduction of raffinose oligosaccharides in meat analogues
title_sort fermentation of faba bean flour as a pre treatment in high moisture extrusion for simultaneous fortification of vitamin b12 and reduction of raffinose oligosaccharides in meat analogues
topic Fermentation
Vitamin B12
Raffinose family oligosaccharides
High-moisture extrusion
Wet mass feeding
Faba bean
url http://www.sciencedirect.com/science/article/pii/S2666833524002211
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