Effects of anti-CD4 antibody treatment on calcium ions influx in peanut-sensitized C3H/HeJ mice
The precise mechanism underlying the effects of anti-CD4 antibody and calcium ions (Ca2+) in peanut allergy remains unknown. C3H/HeJ mice sensitized with peanut protein extract (PPE) were injected with anti-CD4 antibodies for 4 weeks. Stimulation with PPE increased the specific immunoglobulin (IgE),...
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Tsinghua University Press
2023-05-01
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001975 |
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author | Junjuan Wang Cui Zhou Shiwen Han Zainabu Majid Na Sun Huilian Che |
author_facet | Junjuan Wang Cui Zhou Shiwen Han Zainabu Majid Na Sun Huilian Che |
author_sort | Junjuan Wang |
collection | DOAJ |
description | The precise mechanism underlying the effects of anti-CD4 antibody and calcium ions (Ca2+) in peanut allergy remains unknown. C3H/HeJ mice sensitized with peanut protein extract (PPE) were injected with anti-CD4 antibodies for 4 weeks. Stimulation with PPE increased the specific immunoglobulin (IgE), cytokine, histamine, and mMcp-1 levels, upregulated decorin (Dcn) expression, induced Ca2+ inflow in the spleen, and augmented the expression of the transcription factors GATA-3 and Foxp3, which resulted in Th2 and Treg cell activation. Notably, the Ca2+ levels were positively correlated with the histamine, interleukin (IL)-4, IL-5, and IL-13 levels, and negatively correlated with IL-10 levels. However, administration of anti-CD4 antibodies markedly alleviated allergic symptoms, activated T cells, and reduced Ca2+ inflow, cytokine, histamine, mMcp-1, and the IgHG3, CXCL12, MMP2 and FABP4 gene. Our results indicated that anti-CD4 antibodies can ameliorate PPE-induced allergy, which is probably related to the suppression of Ca2+ inflow, and inhibiting histamine, cytokine and IgHG3, CXCL12, MMP2, and FABP4, thus exerting a protective effect against PPE-sensitized food allergy. |
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id | doaj-art-3753fa9c64a6498aa5372f93009ae239 |
institution | Kabale University |
issn | 2213-4530 |
language | English |
publishDate | 2023-05-01 |
publisher | Tsinghua University Press |
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series | Food Science and Human Wellness |
spelling | doaj-art-3753fa9c64a6498aa5372f93009ae2392025-02-03T06:53:35ZengTsinghua University PressFood Science and Human Wellness2213-45302023-05-01123765773Effects of anti-CD4 antibody treatment on calcium ions influx in peanut-sensitized C3H/HeJ miceJunjuan Wang0Cui Zhou1Shiwen Han2Zainabu Majid3Na Sun4Huilian Che5Key Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaSchool of Public Health, Capital Medical University, Beijing100069, ChinaKey Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaKey Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaKey Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Corresponding author at: College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, Beijing 100083, China.The precise mechanism underlying the effects of anti-CD4 antibody and calcium ions (Ca2+) in peanut allergy remains unknown. C3H/HeJ mice sensitized with peanut protein extract (PPE) were injected with anti-CD4 antibodies for 4 weeks. Stimulation with PPE increased the specific immunoglobulin (IgE), cytokine, histamine, and mMcp-1 levels, upregulated decorin (Dcn) expression, induced Ca2+ inflow in the spleen, and augmented the expression of the transcription factors GATA-3 and Foxp3, which resulted in Th2 and Treg cell activation. Notably, the Ca2+ levels were positively correlated with the histamine, interleukin (IL)-4, IL-5, and IL-13 levels, and negatively correlated with IL-10 levels. However, administration of anti-CD4 antibodies markedly alleviated allergic symptoms, activated T cells, and reduced Ca2+ inflow, cytokine, histamine, mMcp-1, and the IgHG3, CXCL12, MMP2 and FABP4 gene. Our results indicated that anti-CD4 antibodies can ameliorate PPE-induced allergy, which is probably related to the suppression of Ca2+ inflow, and inhibiting histamine, cytokine and IgHG3, CXCL12, MMP2, and FABP4, thus exerting a protective effect against PPE-sensitized food allergy.http://www.sciencedirect.com/science/article/pii/S2213453022001975Calcium ionsAnti-CD4C3H/HeJ micePeanutAllergy |
spellingShingle | Junjuan Wang Cui Zhou Shiwen Han Zainabu Majid Na Sun Huilian Che Effects of anti-CD4 antibody treatment on calcium ions influx in peanut-sensitized C3H/HeJ mice Food Science and Human Wellness Calcium ions Anti-CD4 C3H/HeJ mice Peanut Allergy |
title | Effects of anti-CD4 antibody treatment on calcium ions influx in peanut-sensitized C3H/HeJ mice |
title_full | Effects of anti-CD4 antibody treatment on calcium ions influx in peanut-sensitized C3H/HeJ mice |
title_fullStr | Effects of anti-CD4 antibody treatment on calcium ions influx in peanut-sensitized C3H/HeJ mice |
title_full_unstemmed | Effects of anti-CD4 antibody treatment on calcium ions influx in peanut-sensitized C3H/HeJ mice |
title_short | Effects of anti-CD4 antibody treatment on calcium ions influx in peanut-sensitized C3H/HeJ mice |
title_sort | effects of anti cd4 antibody treatment on calcium ions influx in peanut sensitized c3h hej mice |
topic | Calcium ions Anti-CD4 C3H/HeJ mice Peanut Allergy |
url | http://www.sciencedirect.com/science/article/pii/S2213453022001975 |
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