Identification of differentially expressed non-coding RNAs in the plasma of women with preterm birth
This study aimed to identify differentially expressed non-coding RNAs (ncRNAs) associated with preterm birth (PTB) and determine biological pathways being influenced in the context of PTB. We processed cell-free RNA sequencing data and identified seventeen differentially expressed (DE) ncRNAs that c...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | RNA Biology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15476286.2024.2449278 |
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| author | Waqasuddin Khan Samiah Kanwar Mohammad Mohsin Mannan Furqan Kabir Naveed Iqbal Mehdia Nadeem Rajab Ali Syeda Rehana Zia Sharmeen Mian Fatima Aziz Sahrish Muneer Adil Kalam Akram Hussain Iqra Javed Muhammad Farrukh Qazi Javairia Khalid Muhammad Imran Nisar Fyezah Jehan |
| author_facet | Waqasuddin Khan Samiah Kanwar Mohammad Mohsin Mannan Furqan Kabir Naveed Iqbal Mehdia Nadeem Rajab Ali Syeda Rehana Zia Sharmeen Mian Fatima Aziz Sahrish Muneer Adil Kalam Akram Hussain Iqra Javed Muhammad Farrukh Qazi Javairia Khalid Muhammad Imran Nisar Fyezah Jehan |
| author_sort | Waqasuddin Khan |
| collection | DOAJ |
| description | This study aimed to identify differentially expressed non-coding RNAs (ncRNAs) associated with preterm birth (PTB) and determine biological pathways being influenced in the context of PTB. We processed cell-free RNA sequencing data and identified seventeen differentially expressed (DE) ncRNAs that could be involved in the onset of PTB. Per the validation via customized RT-qPCR, the recorded variations in expressions of eleven ncRNAs were concordant with the in-silico analyses. The results of this study provide insights into the role of DE ncRNAs and their impact on pregnancy-related biological pathways that could lead to PTB. Further studies are required to elucidate the precise mechanisms by which these DE ncRNAs contribute to adverse pregnancy outcomes (APOs) and their potential as diagnostic biomarkers. |
| format | Article |
| id | doaj-art-3752d36435cf4a1e8dffae34282712cd |
| institution | Kabale University |
| issn | 1547-6286 1555-8584 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | RNA Biology |
| spelling | doaj-art-3752d36435cf4a1e8dffae34282712cd2025-01-13T16:36:25ZengTaylor & Francis GroupRNA Biology1547-62861555-85842025-12-012211810.1080/15476286.2024.2449278Identification of differentially expressed non-coding RNAs in the plasma of women with preterm birthWaqasuddin Khan0Samiah Kanwar1Mohammad Mohsin Mannan2Furqan Kabir3Naveed Iqbal4Mehdia Nadeem Rajab Ali5Syeda Rehana Zia6Sharmeen Mian7Fatima Aziz8Sahrish Muneer9Adil Kalam10Akram Hussain11Iqra Javed12Muhammad Farrukh Qazi13Javairia Khalid14Muhammad Imran Nisar15Fyezah Jehan16Biorepository and Omics Research Group, Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanBiorepository and Omics Research Group, Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanBiorepository and Omics Research Group, Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanInfectious Diseases Research Lab (IDRL), Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanBiorepository and Omics Research Group, Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanBiorepository and Omics Research Group, Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanBiorepository and Omics Research Group, Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanBiorepository and Omics Research Group, Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanInfectious Diseases Research Lab (IDRL), Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanInfectious Diseases Research Lab (IDRL), Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanInfectious Diseases Research Lab (IDRL), Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanInfectious Diseases Research Lab (IDRL), Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanInfectious Diseases Research Lab (IDRL), Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanBiorepository and Omics Research Group, Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanBiorepository and Omics Research Group, Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanBiorepository and Omics Research Group, Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanBiorepository and Omics Research Group, Department of Pediatrics and Child Health, Faculty of Health Sciences, Medical College, The Aga Khan University, Karachi, PakistanThis study aimed to identify differentially expressed non-coding RNAs (ncRNAs) associated with preterm birth (PTB) and determine biological pathways being influenced in the context of PTB. We processed cell-free RNA sequencing data and identified seventeen differentially expressed (DE) ncRNAs that could be involved in the onset of PTB. Per the validation via customized RT-qPCR, the recorded variations in expressions of eleven ncRNAs were concordant with the in-silico analyses. The results of this study provide insights into the role of DE ncRNAs and their impact on pregnancy-related biological pathways that could lead to PTB. Further studies are required to elucidate the precise mechanisms by which these DE ncRNAs contribute to adverse pregnancy outcomes (APOs) and their potential as diagnostic biomarkers.https://www.tandfonline.com/doi/10.1080/15476286.2024.2449278Preterm birthCf-RNA-seqncRNAsTAC assayannotations |
| spellingShingle | Waqasuddin Khan Samiah Kanwar Mohammad Mohsin Mannan Furqan Kabir Naveed Iqbal Mehdia Nadeem Rajab Ali Syeda Rehana Zia Sharmeen Mian Fatima Aziz Sahrish Muneer Adil Kalam Akram Hussain Iqra Javed Muhammad Farrukh Qazi Javairia Khalid Muhammad Imran Nisar Fyezah Jehan Identification of differentially expressed non-coding RNAs in the plasma of women with preterm birth RNA Biology Preterm birth Cf-RNA-seq ncRNAs TAC assay annotations |
| title | Identification of differentially expressed non-coding RNAs in the plasma of women with preterm birth |
| title_full | Identification of differentially expressed non-coding RNAs in the plasma of women with preterm birth |
| title_fullStr | Identification of differentially expressed non-coding RNAs in the plasma of women with preterm birth |
| title_full_unstemmed | Identification of differentially expressed non-coding RNAs in the plasma of women with preterm birth |
| title_short | Identification of differentially expressed non-coding RNAs in the plasma of women with preterm birth |
| title_sort | identification of differentially expressed non coding rnas in the plasma of women with preterm birth |
| topic | Preterm birth Cf-RNA-seq ncRNAs TAC assay annotations |
| url | https://www.tandfonline.com/doi/10.1080/15476286.2024.2449278 |
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