Chemical Characterization, Antioxidant and Enzyme‐Inhibitory Activities of Different Extracts from Three Phlomis Species

Phlomis species (family Lamiaceae) are highly valued as food and herbal medicine. The present study is designed to investigate the chemical composition and antioxidant and enzyme inhibitory activities of extracts from P. fruticosa, P. herba‐venti, and P. kurdica aerial parts. Different classes of me...

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Main Authors: Sakina Yagi, Gokhan Zengin, Mehmet Veysi Cetiz, Zoltán Cziáky, József Jeko, Muammer Bahşi, Uğur Çakılcıoğlu, Halbay Turumtay, Abdurrahman Aktumsek
Format: Article
Language:English
Published: Wiley-VCH 2025-08-01
Series:ChemistryOpen
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Online Access:https://doi.org/10.1002/open.202500004
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author Sakina Yagi
Gokhan Zengin
Mehmet Veysi Cetiz
Zoltán Cziáky
József Jeko
Muammer Bahşi
Uğur Çakılcıoğlu
Halbay Turumtay
Abdurrahman Aktumsek
author_facet Sakina Yagi
Gokhan Zengin
Mehmet Veysi Cetiz
Zoltán Cziáky
József Jeko
Muammer Bahşi
Uğur Çakılcıoğlu
Halbay Turumtay
Abdurrahman Aktumsek
author_sort Sakina Yagi
collection DOAJ
description Phlomis species (family Lamiaceae) are highly valued as food and herbal medicine. The present study is designed to investigate the chemical composition and antioxidant and enzyme inhibitory activities of extracts from P. fruticosa, P. herba‐venti, and P. kurdica aerial parts. Different classes of metabolites, including phenolic acids, phenylethanoids, flavonoids, iridoids, organic acids, terpenes, and fatty acids, are identified in the three species, with methanol as the best solvent to recover bioactive compounds from the three species in addition to ethyl acetate for P. kurdica. Around 70% methanol extract of P. herba‐venti exerts the best radical scavenging and ions‐reducing properties, while its methanol extract exhibits the highest acetylcholinesterase inhibitory activity. The ethyl acetate extract of P. fruticosa displays the best chelating power, and its other polar extracts have the highest total antioxidant activity. Furthermore, molecular docking and molecular dynamics simulations have underscored the therapeutic potential of bioactive compounds, including isoverbascoside, samioside, forsythoside B, and hattushoside. In conclusion, the study indicates that these three Phlomis species are a rich source of bioactive molecules with possible therapeutic applications, and the selection of appropriate extraction solvents is crucial for the targeted biological activity.
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institution Kabale University
issn 2191-1363
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series ChemistryOpen
spelling doaj-art-372548b42d6e4dbda53b4ae34505efda2025-08-21T12:05:32ZengWiley-VCHChemistryOpen2191-13632025-08-01148n/an/a10.1002/open.202500004Chemical Characterization, Antioxidant and Enzyme‐Inhibitory Activities of Different Extracts from Three Phlomis SpeciesSakina Yagi0Gokhan Zengin1Mehmet Veysi Cetiz2Zoltán Cziáky3József Jeko4Muammer Bahşi5Uğur Çakılcıoğlu6Halbay Turumtay7Abdurrahman Aktumsek8Department of Botany Faculty of Science University of Khartoum Khartoum 11115 SudanDepartment of Biology Science Faculty Selcuk University 42130 Konya TürkiyeDepartment of Medical Biochemistry Faculty of Medicine Harran University 63290 Sanliurfa TurkeyAgricultural and Molecular Research and Service Institute University of Nyíregyháza 001 Nyíregyháza HU‐4400 HungaryAgricultural and Molecular Research and Service Institute University of Nyíregyháza 001 Nyíregyháza HU‐4400 HungaryFaculty of Education Department of Primary Education Fırat University Elazığ TurkeyPertek Sakine Genç Vocational School Munzur University 62500 Pertek,Tunceli TurkeyDepartment of Energy System Engineering Karadeniz Technical University 61830 Trabzon TurkeyDepartment of Biology Science Faculty Selcuk University 42130 Konya TürkiyePhlomis species (family Lamiaceae) are highly valued as food and herbal medicine. The present study is designed to investigate the chemical composition and antioxidant and enzyme inhibitory activities of extracts from P. fruticosa, P. herba‐venti, and P. kurdica aerial parts. Different classes of metabolites, including phenolic acids, phenylethanoids, flavonoids, iridoids, organic acids, terpenes, and fatty acids, are identified in the three species, with methanol as the best solvent to recover bioactive compounds from the three species in addition to ethyl acetate for P. kurdica. Around 70% methanol extract of P. herba‐venti exerts the best radical scavenging and ions‐reducing properties, while its methanol extract exhibits the highest acetylcholinesterase inhibitory activity. The ethyl acetate extract of P. fruticosa displays the best chelating power, and its other polar extracts have the highest total antioxidant activity. Furthermore, molecular docking and molecular dynamics simulations have underscored the therapeutic potential of bioactive compounds, including isoverbascoside, samioside, forsythoside B, and hattushoside. In conclusion, the study indicates that these three Phlomis species are a rich source of bioactive molecules with possible therapeutic applications, and the selection of appropriate extraction solvents is crucial for the targeted biological activity.https://doi.org/10.1002/open.202500004antioxidantsenzyme inhibitionP. herba‐ventiP. kurdicaPhlomis fruticosaphytoconstituents
spellingShingle Sakina Yagi
Gokhan Zengin
Mehmet Veysi Cetiz
Zoltán Cziáky
József Jeko
Muammer Bahşi
Uğur Çakılcıoğlu
Halbay Turumtay
Abdurrahman Aktumsek
Chemical Characterization, Antioxidant and Enzyme‐Inhibitory Activities of Different Extracts from Three Phlomis Species
ChemistryOpen
antioxidants
enzyme inhibition
P. herba‐venti
P. kurdica
Phlomis fruticosa
phytoconstituents
title Chemical Characterization, Antioxidant and Enzyme‐Inhibitory Activities of Different Extracts from Three Phlomis Species
title_full Chemical Characterization, Antioxidant and Enzyme‐Inhibitory Activities of Different Extracts from Three Phlomis Species
title_fullStr Chemical Characterization, Antioxidant and Enzyme‐Inhibitory Activities of Different Extracts from Three Phlomis Species
title_full_unstemmed Chemical Characterization, Antioxidant and Enzyme‐Inhibitory Activities of Different Extracts from Three Phlomis Species
title_short Chemical Characterization, Antioxidant and Enzyme‐Inhibitory Activities of Different Extracts from Three Phlomis Species
title_sort chemical characterization antioxidant and enzyme inhibitory activities of different extracts from three phlomis species
topic antioxidants
enzyme inhibition
P. herba‐venti
P. kurdica
Phlomis fruticosa
phytoconstituents
url https://doi.org/10.1002/open.202500004
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