The Effect of Salinity on Heavy Metal Tolerance in Two Energy Willow Varieties

This study evaluated the response of two willow varieties, <i>Salix × smithiana</i> Willd. and <i>Salix viminalis</i> L. var. Gigantea, to selected heavy metals and elevated soil salinity, simulating complex environmental conditions during phytoremediation. Plants propagated...

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Main Authors: Kinga Drzewiecka, Zuzanna Kaźmierczak, Magdalena Woźniak, Michał Rybak
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/12/1747
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author Kinga Drzewiecka
Zuzanna Kaźmierczak
Magdalena Woźniak
Michał Rybak
author_facet Kinga Drzewiecka
Zuzanna Kaźmierczak
Magdalena Woźniak
Michał Rybak
author_sort Kinga Drzewiecka
collection DOAJ
description This study evaluated the response of two willow varieties, <i>Salix × smithiana</i> Willd. and <i>Salix viminalis</i> L. var. Gigantea, to selected heavy metals and elevated soil salinity, simulating complex environmental conditions during phytoremediation. Plants propagated from stem cuttings were cultivated in pots under field conditions in soil artificially contaminated with a mixture of Cd, Ni, Cu, Zn, and Pb salts at two concentration levels representing lower and higher guideline thresholds. Sodium chloride was added to induce salinity stress. <i>S. × smithiana</i> exhibited enhanced growth under combined metal and salinity stress, suggesting efficient tolerance mechanisms. This was reflected in elevated relative water content (RWC) and increased accumulation of Zn and Cd in shoots. In contrast, Gigantea showed growth inhibition and primarily sequestered metals in roots, indicating a stress-avoidance strategy and reduced metal translocation. While salinity alone negatively affected both varieties, its combination with metals mitigated growth reduction in <i>S. × smithiana</i>, possibly due to improved ion homeostasis or cross-tolerance. Zn and Cd displayed the highest bioconcentration and mobility. Based on bioconcentration factor (BCF) and translocation factor (TF), <i>S. × smithiana</i> appears suitable for phytoextraction, whereas <i>S. viminalis</i> var. Gigantea appears suitable for phytostabilization. These results support species-specific approaches to phytoremediation in multi-contaminant environments.
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spelling doaj-art-3721e91e1cba40d78ef727c5dc0cb4142025-08-20T03:27:42ZengMDPI AGPlants2223-77472025-06-011412174710.3390/plants14121747The Effect of Salinity on Heavy Metal Tolerance in Two Energy Willow VarietiesKinga Drzewiecka0Zuzanna Kaźmierczak1Magdalena Woźniak2Michał Rybak3Department of Chemistry, Faculty of Forestry and Wood Technology, Poznań University of Life Sciences, Wojska Polskiego 75, 60-625 Poznań, PolandDepartment of Chemistry, Faculty of Forestry and Wood Technology, Poznań University of Life Sciences, Wojska Polskiego 75, 60-625 Poznań, PolandDepartment of Chemistry, Faculty of Forestry and Wood Technology, Poznań University of Life Sciences, Wojska Polskiego 75, 60-625 Poznań, PolandDepartment of Water Protection, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznań, PolandThis study evaluated the response of two willow varieties, <i>Salix × smithiana</i> Willd. and <i>Salix viminalis</i> L. var. Gigantea, to selected heavy metals and elevated soil salinity, simulating complex environmental conditions during phytoremediation. Plants propagated from stem cuttings were cultivated in pots under field conditions in soil artificially contaminated with a mixture of Cd, Ni, Cu, Zn, and Pb salts at two concentration levels representing lower and higher guideline thresholds. Sodium chloride was added to induce salinity stress. <i>S. × smithiana</i> exhibited enhanced growth under combined metal and salinity stress, suggesting efficient tolerance mechanisms. This was reflected in elevated relative water content (RWC) and increased accumulation of Zn and Cd in shoots. In contrast, Gigantea showed growth inhibition and primarily sequestered metals in roots, indicating a stress-avoidance strategy and reduced metal translocation. While salinity alone negatively affected both varieties, its combination with metals mitigated growth reduction in <i>S. × smithiana</i>, possibly due to improved ion homeostasis or cross-tolerance. Zn and Cd displayed the highest bioconcentration and mobility. Based on bioconcentration factor (BCF) and translocation factor (TF), <i>S. × smithiana</i> appears suitable for phytoextraction, whereas <i>S. viminalis</i> var. Gigantea appears suitable for phytostabilization. These results support species-specific approaches to phytoremediation in multi-contaminant environments.https://www.mdpi.com/2223-7747/14/12/1747environmental stressmetal uptakeosmotic stressphytoremediation<i>Salix</i> sp.
spellingShingle Kinga Drzewiecka
Zuzanna Kaźmierczak
Magdalena Woźniak
Michał Rybak
The Effect of Salinity on Heavy Metal Tolerance in Two Energy Willow Varieties
Plants
environmental stress
metal uptake
osmotic stress
phytoremediation
<i>Salix</i> sp.
title The Effect of Salinity on Heavy Metal Tolerance in Two Energy Willow Varieties
title_full The Effect of Salinity on Heavy Metal Tolerance in Two Energy Willow Varieties
title_fullStr The Effect of Salinity on Heavy Metal Tolerance in Two Energy Willow Varieties
title_full_unstemmed The Effect of Salinity on Heavy Metal Tolerance in Two Energy Willow Varieties
title_short The Effect of Salinity on Heavy Metal Tolerance in Two Energy Willow Varieties
title_sort effect of salinity on heavy metal tolerance in two energy willow varieties
topic environmental stress
metal uptake
osmotic stress
phytoremediation
<i>Salix</i> sp.
url https://www.mdpi.com/2223-7747/14/12/1747
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