Identification of Characteristic Flavor Compounds and Quality Evaluation of <i>Butyriboletus roseoflavus</i> from Different Regions in Yunnan

<i>Butyriboletus roseoflavus</i> is a rare wild edible mushroom. Yet, the relationship between its chemical composition and quality, as well as the influence of geographic origin on its flavor profile, remains unclear. In this study, ultra-performance liquid chromatography–tandem mass sp...

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Main Authors: Ling Song, Qiming Zhao, Xuebin Shao, Xiangqian Lv, Juan Lu, Ruiping Luo, Yurong Liu, Xing Zhou, Qiang Li, Mingying Gui
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Foods
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Online Access:https://www.mdpi.com/2304-8158/14/10/1676
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author Ling Song
Qiming Zhao
Xuebin Shao
Xiangqian Lv
Juan Lu
Ruiping Luo
Yurong Liu
Xing Zhou
Qiang Li
Mingying Gui
author_facet Ling Song
Qiming Zhao
Xuebin Shao
Xiangqian Lv
Juan Lu
Ruiping Luo
Yurong Liu
Xing Zhou
Qiang Li
Mingying Gui
author_sort Ling Song
collection DOAJ
description <i>Butyriboletus roseoflavus</i> is a rare wild edible mushroom. Yet, the relationship between its chemical composition and quality, as well as the influence of geographic origin on its flavor profile, remains unclear. In this study, ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) and headspace solid-phase microextraction coupled with gas chromatography–mass spectrometry (HS-SPME-GC-MS) were used to investigate flavor differences and influencing factors among samples from different regions. Seventeen key volatile compounds (OAV > 1) were identified, with α-pinene, styrene, octanal, 1,3,5-trithiane, and 2,4-undecadienal being the primary aroma contributors. Six characteristic taste-active compounds (TAV > 1) were detected, among which Glu, Ala, and His played dominant roles. Differential metabolites were mainly enriched in nucleotides and their derivatives, suggesting their importance in environmental adaptation and quality formation. Correlation analysis revealed that the abundance of key metabolites was closely related to geographic origin: temperature, humidity, light intensity, and CO<sub>2</sub> concentration mainly influenced aroma variation, while taste differences were associated with soil electrical conductivity and microclimatic changes mediated by altitude. These findings provide a comprehensive understanding of the flavor characteristics of <i>B. roseoflavus</i> and offer a theoretical basis for its future processing and utilization.
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spelling doaj-art-371fbda9da184ee1b6a3cc66d0c4c4eb2025-08-20T02:33:55ZengMDPI AGFoods2304-81582025-05-011410167610.3390/foods14101676Identification of Characteristic Flavor Compounds and Quality Evaluation of <i>Butyriboletus roseoflavus</i> from Different Regions in YunnanLing Song0Qiming Zhao1Xuebin Shao2Xiangqian Lv3Juan Lu4Ruiping Luo5Yurong Liu6Xing Zhou7Qiang Li8Mingying Gui9College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, ChinaThe State-Owned Forest Farm in Longyang District Baoshan City, Baoshan 678000, ChinaLibrary, Yunnan Agricultural University, Kunming 650201, ChinaYunnan Plateau Characteristic Agricultural Industry Research Institute, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaSchool of Food and Biological Engineering, Chengdu University, Chengdu 610106, ChinaYunnan Plateau Characteristic Agricultural Industry Research Institute, Yunnan Agricultural University, Kunming 650201, China<i>Butyriboletus roseoflavus</i> is a rare wild edible mushroom. Yet, the relationship between its chemical composition and quality, as well as the influence of geographic origin on its flavor profile, remains unclear. In this study, ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) and headspace solid-phase microextraction coupled with gas chromatography–mass spectrometry (HS-SPME-GC-MS) were used to investigate flavor differences and influencing factors among samples from different regions. Seventeen key volatile compounds (OAV > 1) were identified, with α-pinene, styrene, octanal, 1,3,5-trithiane, and 2,4-undecadienal being the primary aroma contributors. Six characteristic taste-active compounds (TAV > 1) were detected, among which Glu, Ala, and His played dominant roles. Differential metabolites were mainly enriched in nucleotides and their derivatives, suggesting their importance in environmental adaptation and quality formation. Correlation analysis revealed that the abundance of key metabolites was closely related to geographic origin: temperature, humidity, light intensity, and CO<sub>2</sub> concentration mainly influenced aroma variation, while taste differences were associated with soil electrical conductivity and microclimatic changes mediated by altitude. These findings provide a comprehensive understanding of the flavor characteristics of <i>B. roseoflavus</i> and offer a theoretical basis for its future processing and utilization.https://www.mdpi.com/2304-8158/14/10/1676edible mushroomaromatastequalitygeographic origin
spellingShingle Ling Song
Qiming Zhao
Xuebin Shao
Xiangqian Lv
Juan Lu
Ruiping Luo
Yurong Liu
Xing Zhou
Qiang Li
Mingying Gui
Identification of Characteristic Flavor Compounds and Quality Evaluation of <i>Butyriboletus roseoflavus</i> from Different Regions in Yunnan
Foods
edible mushroom
aroma
taste
quality
geographic origin
title Identification of Characteristic Flavor Compounds and Quality Evaluation of <i>Butyriboletus roseoflavus</i> from Different Regions in Yunnan
title_full Identification of Characteristic Flavor Compounds and Quality Evaluation of <i>Butyriboletus roseoflavus</i> from Different Regions in Yunnan
title_fullStr Identification of Characteristic Flavor Compounds and Quality Evaluation of <i>Butyriboletus roseoflavus</i> from Different Regions in Yunnan
title_full_unstemmed Identification of Characteristic Flavor Compounds and Quality Evaluation of <i>Butyriboletus roseoflavus</i> from Different Regions in Yunnan
title_short Identification of Characteristic Flavor Compounds and Quality Evaluation of <i>Butyriboletus roseoflavus</i> from Different Regions in Yunnan
title_sort identification of characteristic flavor compounds and quality evaluation of i butyriboletus roseoflavus i from different regions in yunnan
topic edible mushroom
aroma
taste
quality
geographic origin
url https://www.mdpi.com/2304-8158/14/10/1676
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