Long-lived memory B-cell responses following BCG vaccination.
The role of T-cells in immunity against Mycobacterium tuberculosis (M. tuberculosis) infection has been extensively studied, however, that of B-cells still remains comparatively unexplored. In this study, we determined the presence and frequencies of mycobacteria-specific memory B-cells (MBCs) in pe...
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0051381&type=printable |
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| author | Ismail Sebina Jacqueline M Cliff Steven G Smith Sarah Nogaro Emily L Webb Eleanor M Riley Hazel M Dockrell Alison M Elliott Julius C R Hafalla Stephen Cose |
| author_facet | Ismail Sebina Jacqueline M Cliff Steven G Smith Sarah Nogaro Emily L Webb Eleanor M Riley Hazel M Dockrell Alison M Elliott Julius C R Hafalla Stephen Cose |
| author_sort | Ismail Sebina |
| collection | DOAJ |
| description | The role of T-cells in immunity against Mycobacterium tuberculosis (M. tuberculosis) infection has been extensively studied, however, that of B-cells still remains comparatively unexplored. In this study, we determined the presence and frequencies of mycobacteria-specific memory B-cells (MBCs) in peripheral blood from clinically healthy, Bacillus Calmette Guerin (BCG) vaccinated (n = 79) and unvaccinated (n = 14) donors. Purified protein derivative (PPD)-specific MBCs were present in most donors (both vaccinated and unvaccinated) but their frequencies were significantly higher in vaccinated than in unvaccinated donors. MBCs specific for other mycobacterial antigens [antigen-85A (Ag85A), antigen-85B (Ag85B), 6 kDalton early secretory antigenic target (ESAT-6) and the 10 kDalton-culture filtrate protein (CFP-10)] were less prevalent than those recognising PPD. Furthermore, PPD-specific MBCs were detected in BCG vaccinated donors without ESAT-6 and CFP-10 specific responses. Together, these results indicate that BCG vaccination induces long-lived MBC responses. Similar patterns of response were seen when we examined mycobacteria-specific antibody and T-cell responses in these donors. Our data show for the first time that BCG vaccination elicits long-lived mycobacteria-specific MBC responses in healthy individuals, suggesting a more substantial role of B-cells in the response to BCG and other mycobacterial infections than previously thought. |
| format | Article |
| id | doaj-art-371d2245b60c43a6833ba286ab54f6b9 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-371d2245b60c43a6833ba286ab54f6b92025-08-20T02:30:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5138110.1371/journal.pone.0051381Long-lived memory B-cell responses following BCG vaccination.Ismail SebinaJacqueline M CliffSteven G SmithSarah NogaroEmily L WebbEleanor M RileyHazel M DockrellAlison M ElliottJulius C R HafallaStephen CoseThe role of T-cells in immunity against Mycobacterium tuberculosis (M. tuberculosis) infection has been extensively studied, however, that of B-cells still remains comparatively unexplored. In this study, we determined the presence and frequencies of mycobacteria-specific memory B-cells (MBCs) in peripheral blood from clinically healthy, Bacillus Calmette Guerin (BCG) vaccinated (n = 79) and unvaccinated (n = 14) donors. Purified protein derivative (PPD)-specific MBCs were present in most donors (both vaccinated and unvaccinated) but their frequencies were significantly higher in vaccinated than in unvaccinated donors. MBCs specific for other mycobacterial antigens [antigen-85A (Ag85A), antigen-85B (Ag85B), 6 kDalton early secretory antigenic target (ESAT-6) and the 10 kDalton-culture filtrate protein (CFP-10)] were less prevalent than those recognising PPD. Furthermore, PPD-specific MBCs were detected in BCG vaccinated donors without ESAT-6 and CFP-10 specific responses. Together, these results indicate that BCG vaccination induces long-lived MBC responses. Similar patterns of response were seen when we examined mycobacteria-specific antibody and T-cell responses in these donors. Our data show for the first time that BCG vaccination elicits long-lived mycobacteria-specific MBC responses in healthy individuals, suggesting a more substantial role of B-cells in the response to BCG and other mycobacterial infections than previously thought.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0051381&type=printable |
| spellingShingle | Ismail Sebina Jacqueline M Cliff Steven G Smith Sarah Nogaro Emily L Webb Eleanor M Riley Hazel M Dockrell Alison M Elliott Julius C R Hafalla Stephen Cose Long-lived memory B-cell responses following BCG vaccination. PLoS ONE |
| title | Long-lived memory B-cell responses following BCG vaccination. |
| title_full | Long-lived memory B-cell responses following BCG vaccination. |
| title_fullStr | Long-lived memory B-cell responses following BCG vaccination. |
| title_full_unstemmed | Long-lived memory B-cell responses following BCG vaccination. |
| title_short | Long-lived memory B-cell responses following BCG vaccination. |
| title_sort | long lived memory b cell responses following bcg vaccination |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0051381&type=printable |
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