Effects of Omega-3-Rich Pork Lard on Serum Lipid Profile and Gut Microbiome in C57BL/6NJ Mice

Background and Aims. Hyperlipidemia is a risk factor for cardiovascular diseases. This study is aimed at investigating the effects of consuming omega-3-rich pork lard on the serum lipid profile and gut microbiome of the mice model. Methods and Results. We divided 23 C57BL/6NJ males (16-week-old) int...

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Main Authors: Anantawat Koontanatechanon, Manoosak Wongphatcharachai, Nutthawan Nonthabenjawan, Pichaya Jariyahatthakij, Thanchanok Khorporn, Wanpeuch Parnsen, Benjawan Keattisin, Pattarin Leksrisompong, Pairat Srichana, Sattrachai Prasopdee, Sittiruk Roytrakul, Kusuma Sriyakul, Veerachai Thitapakorn, Kammal Kumar Pawa
Format: Article
Language:English
Published: Wiley 2022-01-01
Series:International Journal of Food Science
Online Access:http://dx.doi.org/10.1155/2022/9269968
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author Anantawat Koontanatechanon
Manoosak Wongphatcharachai
Nutthawan Nonthabenjawan
Pichaya Jariyahatthakij
Thanchanok Khorporn
Wanpeuch Parnsen
Benjawan Keattisin
Pattarin Leksrisompong
Pairat Srichana
Sattrachai Prasopdee
Sittiruk Roytrakul
Kusuma Sriyakul
Veerachai Thitapakorn
Kammal Kumar Pawa
author_facet Anantawat Koontanatechanon
Manoosak Wongphatcharachai
Nutthawan Nonthabenjawan
Pichaya Jariyahatthakij
Thanchanok Khorporn
Wanpeuch Parnsen
Benjawan Keattisin
Pattarin Leksrisompong
Pairat Srichana
Sattrachai Prasopdee
Sittiruk Roytrakul
Kusuma Sriyakul
Veerachai Thitapakorn
Kammal Kumar Pawa
author_sort Anantawat Koontanatechanon
collection DOAJ
description Background and Aims. Hyperlipidemia is a risk factor for cardiovascular diseases. This study is aimed at investigating the effects of consuming omega-3-rich pork lard on the serum lipid profile and gut microbiome of the mice model. Methods and Results. We divided 23 C57BL/6NJ males (16-week-old) into 3 groups, and each group received either a control diet, a high-fat diet of coconut oil (coconut oil), or a high-fat diet of omega-3-rich pork lard (omega lard) for 28 days. Thereafter, fasting serum lipids and fecal microbiomes were analyzed. The serum cholesterol, triglyceride, and LDL levels of the omega lard-treated group were significantly reduced compared to the coconut oil-treated group (P<0.05). However, the microbiome analysis revealed a significant increase in the abundance of Lachnospiraceae in the omega lard-treated group compared to the coconut oil-treated group (P<0.05). Furthermore, Spearman’s correlation analysis revealed that the increased serum lipid content was positively correlated with the abundance of Bacteroidaceae (P<0.05) and negatively correlated with the abundance of Lachnospiraceae (P<0.05). Conclusions. These findings suggested that omega-3-rich pork lard altered the serum lipid profile and gut microbiome in the mice model. Practical Application. The excellent protection offered by omega-3-rich pork lard against hyperlipidemia indicated that pork lard could be used as alternative cooking oil for health-conscious individuals. It could also be introduced as a functional ingredient for patients with hyperlipidemia.
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spelling doaj-art-3710dd8ad57b428ba9be90e4bfef94122025-08-20T02:07:45ZengWileyInternational Journal of Food Science2314-57652022-01-01202210.1155/2022/9269968Effects of Omega-3-Rich Pork Lard on Serum Lipid Profile and Gut Microbiome in C57BL/6NJ MiceAnantawat Koontanatechanon0Manoosak Wongphatcharachai1Nutthawan Nonthabenjawan2Pichaya Jariyahatthakij3Thanchanok Khorporn4Wanpeuch Parnsen5Benjawan Keattisin6Pattarin Leksrisompong7Pairat Srichana8Sattrachai Prasopdee9Sittiruk Roytrakul10Kusuma Sriyakul11Veerachai Thitapakorn12Kammal Kumar Pawa13Chulabhorn International College of MedicineFeed Technology OfficeFeed Technology OfficeFeed Technology OfficeFeed Technology OfficeFeed Technology OfficeCPF Food Research & Development CenterCPF Food Research & Development CenterFeed Technology OfficeChulabhorn International College of MedicineNational Center for Genetic Engineering and Biotechnology (BIOTEC)Chulabhorn International College of MedicineChulabhorn International College of MedicineChulabhorn International College of MedicineBackground and Aims. Hyperlipidemia is a risk factor for cardiovascular diseases. This study is aimed at investigating the effects of consuming omega-3-rich pork lard on the serum lipid profile and gut microbiome of the mice model. Methods and Results. We divided 23 C57BL/6NJ males (16-week-old) into 3 groups, and each group received either a control diet, a high-fat diet of coconut oil (coconut oil), or a high-fat diet of omega-3-rich pork lard (omega lard) for 28 days. Thereafter, fasting serum lipids and fecal microbiomes were analyzed. The serum cholesterol, triglyceride, and LDL levels of the omega lard-treated group were significantly reduced compared to the coconut oil-treated group (P<0.05). However, the microbiome analysis revealed a significant increase in the abundance of Lachnospiraceae in the omega lard-treated group compared to the coconut oil-treated group (P<0.05). Furthermore, Spearman’s correlation analysis revealed that the increased serum lipid content was positively correlated with the abundance of Bacteroidaceae (P<0.05) and negatively correlated with the abundance of Lachnospiraceae (P<0.05). Conclusions. These findings suggested that omega-3-rich pork lard altered the serum lipid profile and gut microbiome in the mice model. Practical Application. The excellent protection offered by omega-3-rich pork lard against hyperlipidemia indicated that pork lard could be used as alternative cooking oil for health-conscious individuals. It could also be introduced as a functional ingredient for patients with hyperlipidemia.http://dx.doi.org/10.1155/2022/9269968
spellingShingle Anantawat Koontanatechanon
Manoosak Wongphatcharachai
Nutthawan Nonthabenjawan
Pichaya Jariyahatthakij
Thanchanok Khorporn
Wanpeuch Parnsen
Benjawan Keattisin
Pattarin Leksrisompong
Pairat Srichana
Sattrachai Prasopdee
Sittiruk Roytrakul
Kusuma Sriyakul
Veerachai Thitapakorn
Kammal Kumar Pawa
Effects of Omega-3-Rich Pork Lard on Serum Lipid Profile and Gut Microbiome in C57BL/6NJ Mice
International Journal of Food Science
title Effects of Omega-3-Rich Pork Lard on Serum Lipid Profile and Gut Microbiome in C57BL/6NJ Mice
title_full Effects of Omega-3-Rich Pork Lard on Serum Lipid Profile and Gut Microbiome in C57BL/6NJ Mice
title_fullStr Effects of Omega-3-Rich Pork Lard on Serum Lipid Profile and Gut Microbiome in C57BL/6NJ Mice
title_full_unstemmed Effects of Omega-3-Rich Pork Lard on Serum Lipid Profile and Gut Microbiome in C57BL/6NJ Mice
title_short Effects of Omega-3-Rich Pork Lard on Serum Lipid Profile and Gut Microbiome in C57BL/6NJ Mice
title_sort effects of omega 3 rich pork lard on serum lipid profile and gut microbiome in c57bl 6nj mice
url http://dx.doi.org/10.1155/2022/9269968
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