P-coumaric acid inhibits biofilm formation in pellicles and association with meropenem shows synergistic effect against Acinetobacter baumannii
Acinetobacter baumannii is one of the main causes of hospital infections and has shown broad resistance to antimicrobials. Therefore, effective alternatives are needed to combat this species. From this perspective, this study aimed to search for an antimicrobial alternative using products of natural...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | The Microbe |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950194625000469 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850056910357659648 |
|---|---|
| author | Samily Aquino de Sá Oliveira Danillo Sales Rosa Renata de Faria Silva Souza Amaro Antônio Silva Neto Edilson do Carmo Marins Júnior Márcio Rennan Santos Tavares Maísa Mota Antunes Gustavo Batista de Menezes Vasco Ariston de Carvalho Azevero Flavia Figueira Aburjaile Jackson Roberto Guedes da Silva Almeida Daniel Rodrigo Cavalcante de Araújo Fabiane Rabelo da Costa Batista Carine Rosa Naue Mateus Matiuzzi da Costa |
| author_facet | Samily Aquino de Sá Oliveira Danillo Sales Rosa Renata de Faria Silva Souza Amaro Antônio Silva Neto Edilson do Carmo Marins Júnior Márcio Rennan Santos Tavares Maísa Mota Antunes Gustavo Batista de Menezes Vasco Ariston de Carvalho Azevero Flavia Figueira Aburjaile Jackson Roberto Guedes da Silva Almeida Daniel Rodrigo Cavalcante de Araújo Fabiane Rabelo da Costa Batista Carine Rosa Naue Mateus Matiuzzi da Costa |
| author_sort | Samily Aquino de Sá Oliveira |
| collection | DOAJ |
| description | Acinetobacter baumannii is one of the main causes of hospital infections and has shown broad resistance to antimicrobials. Therefore, effective alternatives are needed to combat this species. From this perspective, this study aimed to search for an antimicrobial alternative using products of natural origin with the potential to combat multidrug-resistant A. baumannii (MDR). Initially, the crude ethanolic extract (CEE) from Hymenaea martiana leaves was prepared, which went through phytochemical screening and compound analysis by high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD). The minimum inhibitory concentration and the minimum bactericidal concentration of CEE, as well as secondary metabolites detected in HPLC, were determined using nine isolates of A. baumannii MDR. The motility and formation of biofilms on solid-liquid interfaces and in pellicles were verified, as well as the interference of secondary metabolites on these virulence factors. The synergistic combination of secondary metabolites and meropenem was also evaluated. In the HPLC-DAD analysis, gallic acid (GA), p-coumaric acid (PA), and rutin were detected. Antimicrobial activity was found for CEE, GA, and PA. PA showed antibiofilm potential, especially on the pellicle. Both phenolic acids showed excellent synergistic activity, reversing resistance to meropenem in some cases. The PA-meropenem combination showed good results, and its use rescues the sensitivity of an already known antibiotic, combined with a substance with antibiofilm activity. These results provide a path for the use of new antimicrobial therapies that may assist in treating A. baumannii. |
| format | Article |
| id | doaj-art-370e511c97344a418ed86ac27b7a80cc |
| institution | DOAJ |
| issn | 2950-1946 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | The Microbe |
| spelling | doaj-art-370e511c97344a418ed86ac27b7a80cc2025-08-20T02:51:35ZengElsevierThe Microbe2950-19462025-03-01610027810.1016/j.microb.2025.100278P-coumaric acid inhibits biofilm formation in pellicles and association with meropenem shows synergistic effect against Acinetobacter baumanniiSamily Aquino de Sá Oliveira0Danillo Sales Rosa1Renata de Faria Silva Souza2Amaro Antônio Silva Neto3Edilson do Carmo Marins Júnior4Márcio Rennan Santos Tavares5Maísa Mota Antunes6Gustavo Batista de Menezes7Vasco Ariston de Carvalho Azevero8Flavia Figueira Aburjaile9Jackson Roberto Guedes da Silva Almeida10Daniel Rodrigo Cavalcante de Araújo11Fabiane Rabelo da Costa Batista12Carine Rosa Naue13Mateus Matiuzzi da Costa14Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco 64770-000, BrazilUniversidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco 64770-000, BrazilUniversidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco 64770-000, BrazilUniversidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco 64770-000, BrazilUniversidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco 64770-000, BrazilUniversidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco 64770-000, BrazilUniversidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais 31270-901, BrazilUniversidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais 31270-901, BrazilUniversidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais 31270-901, BrazilUniversidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais 31270-901, BrazilUniversidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco 64770-000, BrazilInstituto Nacional do Semiárido (INSA), Campina Grande, Paraíba 58434-700, BrazilInstituto Nacional do Semiárido (INSA), Campina Grande, Paraíba 58434-700, BrazilHospital Universitário da Universidade Federal do Vale do São Francisco (HU-UNIVASF), Petrolina, Pernambuco 56304-205, BrazilUniversidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco 64770-000, Brazil; Corresponding author.Acinetobacter baumannii is one of the main causes of hospital infections and has shown broad resistance to antimicrobials. Therefore, effective alternatives are needed to combat this species. From this perspective, this study aimed to search for an antimicrobial alternative using products of natural origin with the potential to combat multidrug-resistant A. baumannii (MDR). Initially, the crude ethanolic extract (CEE) from Hymenaea martiana leaves was prepared, which went through phytochemical screening and compound analysis by high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD). The minimum inhibitory concentration and the minimum bactericidal concentration of CEE, as well as secondary metabolites detected in HPLC, were determined using nine isolates of A. baumannii MDR. The motility and formation of biofilms on solid-liquid interfaces and in pellicles were verified, as well as the interference of secondary metabolites on these virulence factors. The synergistic combination of secondary metabolites and meropenem was also evaluated. In the HPLC-DAD analysis, gallic acid (GA), p-coumaric acid (PA), and rutin were detected. Antimicrobial activity was found for CEE, GA, and PA. PA showed antibiofilm potential, especially on the pellicle. Both phenolic acids showed excellent synergistic activity, reversing resistance to meropenem in some cases. The PA-meropenem combination showed good results, and its use rescues the sensitivity of an already known antibiotic, combined with a substance with antibiofilm activity. These results provide a path for the use of new antimicrobial therapies that may assist in treating A. baumannii.http://www.sciencedirect.com/science/article/pii/S2950194625000469Antimicrobial resistanceSecondary metabolitesHospital infectionsVirulenceAntimicrobial alternativeAntibiofilm activity |
| spellingShingle | Samily Aquino de Sá Oliveira Danillo Sales Rosa Renata de Faria Silva Souza Amaro Antônio Silva Neto Edilson do Carmo Marins Júnior Márcio Rennan Santos Tavares Maísa Mota Antunes Gustavo Batista de Menezes Vasco Ariston de Carvalho Azevero Flavia Figueira Aburjaile Jackson Roberto Guedes da Silva Almeida Daniel Rodrigo Cavalcante de Araújo Fabiane Rabelo da Costa Batista Carine Rosa Naue Mateus Matiuzzi da Costa P-coumaric acid inhibits biofilm formation in pellicles and association with meropenem shows synergistic effect against Acinetobacter baumannii The Microbe Antimicrobial resistance Secondary metabolites Hospital infections Virulence Antimicrobial alternative Antibiofilm activity |
| title | P-coumaric acid inhibits biofilm formation in pellicles and association with meropenem shows synergistic effect against Acinetobacter baumannii |
| title_full | P-coumaric acid inhibits biofilm formation in pellicles and association with meropenem shows synergistic effect against Acinetobacter baumannii |
| title_fullStr | P-coumaric acid inhibits biofilm formation in pellicles and association with meropenem shows synergistic effect against Acinetobacter baumannii |
| title_full_unstemmed | P-coumaric acid inhibits biofilm formation in pellicles and association with meropenem shows synergistic effect against Acinetobacter baumannii |
| title_short | P-coumaric acid inhibits biofilm formation in pellicles and association with meropenem shows synergistic effect against Acinetobacter baumannii |
| title_sort | p coumaric acid inhibits biofilm formation in pellicles and association with meropenem shows synergistic effect against acinetobacter baumannii |
| topic | Antimicrobial resistance Secondary metabolites Hospital infections Virulence Antimicrobial alternative Antibiofilm activity |
| url | http://www.sciencedirect.com/science/article/pii/S2950194625000469 |
| work_keys_str_mv | AT samilyaquinodesaoliveira pcoumaricacidinhibitsbiofilmformationinpelliclesandassociationwithmeropenemshowssynergisticeffectagainstacinetobacterbaumannii AT danillosalesrosa pcoumaricacidinhibitsbiofilmformationinpelliclesandassociationwithmeropenemshowssynergisticeffectagainstacinetobacterbaumannii AT renatadefariasilvasouza pcoumaricacidinhibitsbiofilmformationinpelliclesandassociationwithmeropenemshowssynergisticeffectagainstacinetobacterbaumannii AT amaroantoniosilvaneto pcoumaricacidinhibitsbiofilmformationinpelliclesandassociationwithmeropenemshowssynergisticeffectagainstacinetobacterbaumannii AT edilsondocarmomarinsjunior pcoumaricacidinhibitsbiofilmformationinpelliclesandassociationwithmeropenemshowssynergisticeffectagainstacinetobacterbaumannii AT marciorennansantostavares pcoumaricacidinhibitsbiofilmformationinpelliclesandassociationwithmeropenemshowssynergisticeffectagainstacinetobacterbaumannii AT maisamotaantunes pcoumaricacidinhibitsbiofilmformationinpelliclesandassociationwithmeropenemshowssynergisticeffectagainstacinetobacterbaumannii AT gustavobatistademenezes pcoumaricacidinhibitsbiofilmformationinpelliclesandassociationwithmeropenemshowssynergisticeffectagainstacinetobacterbaumannii AT vascoaristondecarvalhoazevero pcoumaricacidinhibitsbiofilmformationinpelliclesandassociationwithmeropenemshowssynergisticeffectagainstacinetobacterbaumannii AT flaviafigueiraaburjaile pcoumaricacidinhibitsbiofilmformationinpelliclesandassociationwithmeropenemshowssynergisticeffectagainstacinetobacterbaumannii AT jacksonrobertoguedesdasilvaalmeida pcoumaricacidinhibitsbiofilmformationinpelliclesandassociationwithmeropenemshowssynergisticeffectagainstacinetobacterbaumannii AT danielrodrigocavalcantedearaujo pcoumaricacidinhibitsbiofilmformationinpelliclesandassociationwithmeropenemshowssynergisticeffectagainstacinetobacterbaumannii AT fabianerabelodacostabatista pcoumaricacidinhibitsbiofilmformationinpelliclesandassociationwithmeropenemshowssynergisticeffectagainstacinetobacterbaumannii AT carinerosanaue pcoumaricacidinhibitsbiofilmformationinpelliclesandassociationwithmeropenemshowssynergisticeffectagainstacinetobacterbaumannii AT mateusmatiuzzidacosta pcoumaricacidinhibitsbiofilmformationinpelliclesandassociationwithmeropenemshowssynergisticeffectagainstacinetobacterbaumannii |