Three-dimensional spheroid models for cardiovascular biology and pathology
Scaffold-free three-dimensional (3D) cellular spheroid cultures better replicate the in vivo cellular microenvironments of complex tissues than traditional two-dimensional (2D) cell cultures, as they promote more intricate cell-cell and cell-extracellular matrix (ECM) interactions. In the context of...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Mechanobiology in Medicine |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949907025000324 |
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| _version_ | 1849424314435108864 |
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| author | Alanna Krug Gabrielle Inserra Rhonda Drewes Amanda Krajnik Joseph A. Brazzo, III Thomas Mousso Su Chin Heo Yongho Bae |
| author_facet | Alanna Krug Gabrielle Inserra Rhonda Drewes Amanda Krajnik Joseph A. Brazzo, III Thomas Mousso Su Chin Heo Yongho Bae |
| author_sort | Alanna Krug |
| collection | DOAJ |
| description | Scaffold-free three-dimensional (3D) cellular spheroid cultures better replicate the in vivo cellular microenvironments of complex tissues than traditional two-dimensional (2D) cell cultures, as they promote more intricate cell-cell and cell-extracellular matrix (ECM) interactions. In the context of cardiovascular research, 3D spheroids have emerged as valuable models for studying angiogenesis, modeling the cardiac microenvironment, and advancing drug development and cardiac tissue repair. Given that cardiovascular disease remains the leading cause of morbidity worldwide, exploring 3D spheroids as in vitro models in cardiovascular research holds potential for advancing the field. Despite their promise, the experimental potential of 3D spheroids in cardiovascular disease and biology has yet to be realized. Therefore, this review discusses the advantages and limitations of 3D spheroid models for studying angiogenesis and cardiovascular pathobiology, their applications in cardiac drug development and tissue repair, and how these models can advance cardiovascular research. |
| format | Article |
| id | doaj-art-36fa6253733d4c19a433abf50d308437 |
| institution | Kabale University |
| issn | 2949-9070 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Mechanobiology in Medicine |
| spelling | doaj-art-36fa6253733d4c19a433abf50d3084372025-08-20T03:30:14ZengElsevierMechanobiology in Medicine2949-90702025-09-013310014410.1016/j.mbm.2025.100144Three-dimensional spheroid models for cardiovascular biology and pathologyAlanna Krug0Gabrielle Inserra1Rhonda Drewes2Amanda Krajnik3Joseph A. Brazzo, III4Thomas Mousso5Su Chin Heo6Yongho Bae7Department of Pathology and Anatomical Sciences, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USADepartment of Pathology and Anatomical Sciences, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USADepartment of Pathology and Anatomical Sciences, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USADepartment of Pathology and Anatomical Sciences, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USADepartment of Pathology and Anatomical Sciences, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USADepartment of Pathology and Anatomical Sciences, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USADepartment of Orthopedic Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA, USADepartment of Pathology and Anatomical Sciences, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USA; Department of Biomedical Engineering, School of Engineering and Applied Sciences, University at Buffalo, Buffalo, NY, USA; Corresponding author. Department of Pathology and Anatomical Sciences, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USA.Scaffold-free three-dimensional (3D) cellular spheroid cultures better replicate the in vivo cellular microenvironments of complex tissues than traditional two-dimensional (2D) cell cultures, as they promote more intricate cell-cell and cell-extracellular matrix (ECM) interactions. In the context of cardiovascular research, 3D spheroids have emerged as valuable models for studying angiogenesis, modeling the cardiac microenvironment, and advancing drug development and cardiac tissue repair. Given that cardiovascular disease remains the leading cause of morbidity worldwide, exploring 3D spheroids as in vitro models in cardiovascular research holds potential for advancing the field. Despite their promise, the experimental potential of 3D spheroids in cardiovascular disease and biology has yet to be realized. Therefore, this review discusses the advantages and limitations of 3D spheroid models for studying angiogenesis and cardiovascular pathobiology, their applications in cardiac drug development and tissue repair, and how these models can advance cardiovascular research.http://www.sciencedirect.com/science/article/pii/S2949907025000324 |
| spellingShingle | Alanna Krug Gabrielle Inserra Rhonda Drewes Amanda Krajnik Joseph A. Brazzo, III Thomas Mousso Su Chin Heo Yongho Bae Three-dimensional spheroid models for cardiovascular biology and pathology Mechanobiology in Medicine |
| title | Three-dimensional spheroid models for cardiovascular biology and pathology |
| title_full | Three-dimensional spheroid models for cardiovascular biology and pathology |
| title_fullStr | Three-dimensional spheroid models for cardiovascular biology and pathology |
| title_full_unstemmed | Three-dimensional spheroid models for cardiovascular biology and pathology |
| title_short | Three-dimensional spheroid models for cardiovascular biology and pathology |
| title_sort | three dimensional spheroid models for cardiovascular biology and pathology |
| url | http://www.sciencedirect.com/science/article/pii/S2949907025000324 |
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