Pathogenicity and immunogenicity of recombinant methyltransferase-defective vesicular stomatitis virus
To prevent the vesicular stomatitis virus (VSV) disease, using a panel of recombinant VSV (E1764A, S1827A, Y1650A, and F1691A) that were defective in mRNA cap methylation as vaccine to inoculate five-week-old BALB/c female mice through oral route with 1×10<sup>6</sup> PFU, the pathogenic...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2011-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.04.001 |
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| author | ZHANG Xiao-dong SUN Jing LI Jian-rong YU Lian |
| author_facet | ZHANG Xiao-dong SUN Jing LI Jian-rong YU Lian |
| author_sort | ZHANG Xiao-dong |
| collection | DOAJ |
| description | To prevent the vesicular stomatitis virus (VSV) disease, using a panel of recombinant VSV (E1764A, S1827A, Y1650A, and F1691A) that were defective in mRNA cap methylation as vaccine to inoculate five-week-old BALB/c female mice through oral route with 1×10<sup>6</sup> PFU, the pathogenicity and immunogenicity of these viruses were determined. The result showed that recombinant S 1827A, Y1650A, F1691A were attenuated in mice and E1764A was still pathogenic to mice. The immunized mice were challenged with wild type VSV. Mice immunized with S1827A and Y1650A trigged a high level of neutralizing antibody and were protected from virulent challenge. Taken together, the results above demonstrated that methyltransferase (MTase)-defective VSV (S1827A and Y1650A) were not only attenuated in animals, but also exhibited excellent immunogenicity. Therefore, MTase-defective viruses will be good live vaccine candidates against VSV. |
| format | Article |
| id | doaj-art-36f69ab9b33d4a3c9e8e4500df519da4 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2011-07-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-36f69ab9b33d4a3c9e8e4500df519da42025-08-20T03:34:20ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552011-07-013735536210.3785/j.issn.1008-9209.2011.04.00110089209Pathogenicity and immunogenicity of recombinant methyltransferase-defective vesicular stomatitis virusZHANG Xiao-dongSUN JingLI Jian-rongYU LianTo prevent the vesicular stomatitis virus (VSV) disease, using a panel of recombinant VSV (E1764A, S1827A, Y1650A, and F1691A) that were defective in mRNA cap methylation as vaccine to inoculate five-week-old BALB/c female mice through oral route with 1×10<sup>6</sup> PFU, the pathogenicity and immunogenicity of these viruses were determined. The result showed that recombinant S 1827A, Y1650A, F1691A were attenuated in mice and E1764A was still pathogenic to mice. The immunized mice were challenged with wild type VSV. Mice immunized with S1827A and Y1650A trigged a high level of neutralizing antibody and were protected from virulent challenge. Taken together, the results above demonstrated that methyltransferase (MTase)-defective VSV (S1827A and Y1650A) were not only attenuated in animals, but also exhibited excellent immunogenicity. Therefore, MTase-defective viruses will be good live vaccine candidates against VSV.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.04.001vesicular stomatitis virusmethyltransferasepathogenicityimmunogenicity |
| spellingShingle | ZHANG Xiao-dong SUN Jing LI Jian-rong YU Lian Pathogenicity and immunogenicity of recombinant methyltransferase-defective vesicular stomatitis virus 浙江大学学报. 农业与生命科学版 vesicular stomatitis virus methyltransferase pathogenicity immunogenicity |
| title | Pathogenicity and immunogenicity of recombinant methyltransferase-defective vesicular stomatitis virus |
| title_full | Pathogenicity and immunogenicity of recombinant methyltransferase-defective vesicular stomatitis virus |
| title_fullStr | Pathogenicity and immunogenicity of recombinant methyltransferase-defective vesicular stomatitis virus |
| title_full_unstemmed | Pathogenicity and immunogenicity of recombinant methyltransferase-defective vesicular stomatitis virus |
| title_short | Pathogenicity and immunogenicity of recombinant methyltransferase-defective vesicular stomatitis virus |
| title_sort | pathogenicity and immunogenicity of recombinant methyltransferase defective vesicular stomatitis virus |
| topic | vesicular stomatitis virus methyltransferase pathogenicity immunogenicity |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.04.001 |
| work_keys_str_mv | AT zhangxiaodong pathogenicityandimmunogenicityofrecombinantmethyltransferasedefectivevesicularstomatitisvirus AT sunjing pathogenicityandimmunogenicityofrecombinantmethyltransferasedefectivevesicularstomatitisvirus AT lijianrong pathogenicityandimmunogenicityofrecombinantmethyltransferasedefectivevesicularstomatitisvirus AT yulian pathogenicityandimmunogenicityofrecombinantmethyltransferasedefectivevesicularstomatitisvirus |