Polymorphisms in cathepsin b is associated with growth in Asian seabass
This study investigates the relationship between polymorphisms in the cathepsin B (ctsb) gene and body weight in Asian seabass (Lates calcarifer). Despite the economic significance of this species, the genetic factors that influence growth performance in fish remain poorly understood. CTSB, a lysoso...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2025-09-01
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| Series: | Aquaculture and Fisheries |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468550X25000644 |
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| author | Shadame Yeo Joey Wong Y.X. Tay Zituo Yang Le Wang Fei Sun May Lee Yanfei Wen Gen Hua Yue |
| author_facet | Shadame Yeo Joey Wong Y.X. Tay Zituo Yang Le Wang Fei Sun May Lee Yanfei Wen Gen Hua Yue |
| author_sort | Shadame Yeo |
| collection | DOAJ |
| description | This study investigates the relationship between polymorphisms in the cathepsin B (ctsb) gene and body weight in Asian seabass (Lates calcarifer). Despite the economic significance of this species, the genetic factors that influence growth performance in fish remain poorly understood. CTSB, a lysosomal cysteine protease involved in protein degradation and tissue remodelling, is a crucial regulator of growth. We analysed the full-length cDNA of ctsb and mapped it to the major quantitative trait locus (QTL) for growth on linkage group 2 in L. calcarifer. Single nucleotide polymorphisms (SNPs) within ctsb were identified and their associations with body weight were analysed in 298 individuals. Results revealed that SNP1 (C > T) in intron 2 of ctsb was significantly correlated with body weight. Quantitative real-time PCR (qRT-PCR) showed that ctsb was expressed in all tissues, with the highest expression in the gills and kidney of developing seabass. Knockdown/overexpression of ctsb in cell lines respectively promoted/inhibited cell proliferation without altering cell size. These findings suggest that ctsb plays a significant role in growth regulation in Asian seabass. This study provides a potential molecular marker for selecting fast-growing Asian seabass. Future research should focus on identifying causal variants in ctsb associated with accelerated growth, conducting in vivo studies, and exploring related signalling pathways. |
| format | Article |
| id | doaj-art-36e32afe1f4d499d973d400ff8ba6dc8 |
| institution | Kabale University |
| issn | 2468-550X |
| language | English |
| publishDate | 2025-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Aquaculture and Fisheries |
| spelling | doaj-art-36e32afe1f4d499d973d400ff8ba6dc82025-08-22T04:57:05ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2025-09-0110576477010.1016/j.aaf.2025.05.003Polymorphisms in cathepsin b is associated with growth in Asian seabassShadame Yeo0Joey Wong1Y.X. Tay2Zituo Yang3Le Wang4Fei Sun5May Lee6Yanfei Wen7Gen Hua Yue8Temasek Life Sciences Laboratory, National University of Singapore, 117604, SingaporeTemasek Life Sciences Laboratory, National University of Singapore, 117604, SingaporeTemasek Life Sciences Laboratory, National University of Singapore, 117604, SingaporeTemasek Life Sciences Laboratory, National University of Singapore, 117604, SingaporeTemasek Life Sciences Laboratory, National University of Singapore, 117604, SingaporeTemasek Life Sciences Laboratory, National University of Singapore, 117604, SingaporeTemasek Life Sciences Laboratory, National University of Singapore, 117604, SingaporeTemasek Life Sciences Laboratory, National University of Singapore, 117604, SingaporeTemasek Life Sciences Laboratory, National University of Singapore, 117604, Singapore; Department of Biological Sciences, National University of Singapore, 117558, Singapore; Corresponding author. Temasek Life Sciences Laboratory, National University of Singapore, 117604, Singapore.This study investigates the relationship between polymorphisms in the cathepsin B (ctsb) gene and body weight in Asian seabass (Lates calcarifer). Despite the economic significance of this species, the genetic factors that influence growth performance in fish remain poorly understood. CTSB, a lysosomal cysteine protease involved in protein degradation and tissue remodelling, is a crucial regulator of growth. We analysed the full-length cDNA of ctsb and mapped it to the major quantitative trait locus (QTL) for growth on linkage group 2 in L. calcarifer. Single nucleotide polymorphisms (SNPs) within ctsb were identified and their associations with body weight were analysed in 298 individuals. Results revealed that SNP1 (C > T) in intron 2 of ctsb was significantly correlated with body weight. Quantitative real-time PCR (qRT-PCR) showed that ctsb was expressed in all tissues, with the highest expression in the gills and kidney of developing seabass. Knockdown/overexpression of ctsb in cell lines respectively promoted/inhibited cell proliferation without altering cell size. These findings suggest that ctsb plays a significant role in growth regulation in Asian seabass. This study provides a potential molecular marker for selecting fast-growing Asian seabass. Future research should focus on identifying causal variants in ctsb associated with accelerated growth, conducting in vivo studies, and exploring related signalling pathways.http://www.sciencedirect.com/science/article/pii/S2468550X25000644SeabassAquacultureSNPGrowthAssociationBreeding |
| spellingShingle | Shadame Yeo Joey Wong Y.X. Tay Zituo Yang Le Wang Fei Sun May Lee Yanfei Wen Gen Hua Yue Polymorphisms in cathepsin b is associated with growth in Asian seabass Aquaculture and Fisheries Seabass Aquaculture SNP Growth Association Breeding |
| title | Polymorphisms in cathepsin b is associated with growth in Asian seabass |
| title_full | Polymorphisms in cathepsin b is associated with growth in Asian seabass |
| title_fullStr | Polymorphisms in cathepsin b is associated with growth in Asian seabass |
| title_full_unstemmed | Polymorphisms in cathepsin b is associated with growth in Asian seabass |
| title_short | Polymorphisms in cathepsin b is associated with growth in Asian seabass |
| title_sort | polymorphisms in cathepsin b is associated with growth in asian seabass |
| topic | Seabass Aquaculture SNP Growth Association Breeding |
| url | http://www.sciencedirect.com/science/article/pii/S2468550X25000644 |
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