Measuring the Amount of Melatonin in Twelve Medicinal and Edible Plants
Melatonin (Mel) is a conserved chemical that has been found in far-removed evolutionary organisms. Additionally, it has been proposed that Mel is a stand-alone plant growth regulator whose action is presumably similar to that of IAA and may moderate the effects of other plant growth regulators...
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University of Baghdad
2025-04-01
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| Series: | Ibn Al-Haitham Journal for Pure and Applied Sciences |
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| Online Access: | https://jih.uobaghdad.edu.iq/index.php/j/article/view/3600 |
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| author | Hanan H. ALQaragholy Ayyad W. ALShahwany |
| author_facet | Hanan H. ALQaragholy Ayyad W. ALShahwany |
| author_sort | Hanan H. ALQaragholy |
| collection | DOAJ |
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Melatonin (Mel) is a conserved chemical that has been found in far-removed evolutionary organisms. Additionally, it has been proposed that Mel is a stand-alone plant growth regulator whose action is presumably similar to that of IAA and may moderate the effects of other plant growth regulators. Twelve medicinal herbs were divided into parts, and their melatonin concentrations were measured. These plants Ziziphus spina-christi, , Eucalyptus comaldulensis, Melissa officinalis, Alhagi maurorum, Lavandula angustifolia, Vitex agnus-sactus, Piper nigrum, Hordeum vulgare, Matricaria chamomilla, Borago officinalis, Solanum lycopersicum and Citrus aurantium . Methanol was used to extract melatonin from dried plant parts at room temperature for 6 hours. Then, cyclo (18) carbon was used to refine melatonin. Then, melatonin was analyzed with an reversed-phase chromatography )RP-C18(-approved high-performance liquid chromatography system "HPLC "technique with fluorescence detection and Thin layer chromatography "TLC". Melatonin contents in extracts of Z.spina-christi, E. comaldulensis, M. officinalis, A. maurorum, L. angustifolia,V. agnus-sactus, P. nigrum, H. vulgare, M. chamomilla, B. officinalis, S. lycopersicum and C. aurantium were 420.494, 418.831, 409.750, 408.248, 385.145,239.967, 232.299, 220.569,215.804, 201.862, 108.517 and 106.744 ng/g of plant dry weight, respectively. The extract of Z. spina-christi contained the most melatonin (420.494 ng/g of plant dry weight). Not only do Mel concentrations vary between species, but also within variants of the same species. Different environmental influences on plant growth and development during successive stages of seed morphological and physiological development may cause variations in MEL contents.
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| format | Article |
| id | doaj-art-36e1cb7e360e4eee8bcf5d6c0e068847 |
| institution | OA Journals |
| issn | 1609-4042 2521-3407 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | University of Baghdad |
| record_format | Article |
| series | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| spelling | doaj-art-36e1cb7e360e4eee8bcf5d6c0e0688472025-08-20T02:27:15ZengUniversity of BaghdadIbn Al-Haitham Journal for Pure and Applied Sciences1609-40422521-34072025-04-0138210.30526/38.2.3600Measuring the Amount of Melatonin in Twelve Medicinal and Edible PlantsHanan H. ALQaragholy0https://orcid.org/0009-0006-3230-7279Ayyad W. ALShahwany1https://orcid.org/0000-0002-3821-6355Department of Science, College of Basic Education, Wasit University, Kut, Iraq.Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. Melatonin (Mel) is a conserved chemical that has been found in far-removed evolutionary organisms. Additionally, it has been proposed that Mel is a stand-alone plant growth regulator whose action is presumably similar to that of IAA and may moderate the effects of other plant growth regulators. Twelve medicinal herbs were divided into parts, and their melatonin concentrations were measured. These plants Ziziphus spina-christi, , Eucalyptus comaldulensis, Melissa officinalis, Alhagi maurorum, Lavandula angustifolia, Vitex agnus-sactus, Piper nigrum, Hordeum vulgare, Matricaria chamomilla, Borago officinalis, Solanum lycopersicum and Citrus aurantium . Methanol was used to extract melatonin from dried plant parts at room temperature for 6 hours. Then, cyclo (18) carbon was used to refine melatonin. Then, melatonin was analyzed with an reversed-phase chromatography )RP-C18(-approved high-performance liquid chromatography system "HPLC "technique with fluorescence detection and Thin layer chromatography "TLC". Melatonin contents in extracts of Z.spina-christi, E. comaldulensis, M. officinalis, A. maurorum, L. angustifolia,V. agnus-sactus, P. nigrum, H. vulgare, M. chamomilla, B. officinalis, S. lycopersicum and C. aurantium were 420.494, 418.831, 409.750, 408.248, 385.145,239.967, 232.299, 220.569,215.804, 201.862, 108.517 and 106.744 ng/g of plant dry weight, respectively. The extract of Z. spina-christi contained the most melatonin (420.494 ng/g of plant dry weight). Not only do Mel concentrations vary between species, but also within variants of the same species. Different environmental influences on plant growth and development during successive stages of seed morphological and physiological development may cause variations in MEL contents. https://jih.uobaghdad.edu.iq/index.php/j/article/view/3600Melatonin HPLCTLC |
| spellingShingle | Hanan H. ALQaragholy Ayyad W. ALShahwany Measuring the Amount of Melatonin in Twelve Medicinal and Edible Plants Ibn Al-Haitham Journal for Pure and Applied Sciences Melatonin HPLC TLC |
| title | Measuring the Amount of Melatonin in Twelve Medicinal and Edible Plants |
| title_full | Measuring the Amount of Melatonin in Twelve Medicinal and Edible Plants |
| title_fullStr | Measuring the Amount of Melatonin in Twelve Medicinal and Edible Plants |
| title_full_unstemmed | Measuring the Amount of Melatonin in Twelve Medicinal and Edible Plants |
| title_short | Measuring the Amount of Melatonin in Twelve Medicinal and Edible Plants |
| title_sort | measuring the amount of melatonin in twelve medicinal and edible plants |
| topic | Melatonin HPLC TLC |
| url | https://jih.uobaghdad.edu.iq/index.php/j/article/view/3600 |
| work_keys_str_mv | AT hananhalqaragholy measuringtheamountofmelatoninintwelvemedicinalandedibleplants AT ayyadwalshahwany measuringtheamountofmelatoninintwelvemedicinalandedibleplants |