Cell-SELEX Identifies a “Sticky” RNA Aptamer Sequence
Cell-SELEX is performed to select for cell binding aptamers. We employed an additional selection pressure by using RNAse to remove surface-binding aptamers and select for cell-internalizing aptamers. A common RNA sequence was identified from independent cell-SELEX procedures against two different pa...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.1155/2017/4943072 |
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| _version_ | 1849306644900478976 |
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| author | Partha Ray Rebekah R. White |
| author_facet | Partha Ray Rebekah R. White |
| author_sort | Partha Ray |
| collection | DOAJ |
| description | Cell-SELEX is performed to select for cell binding aptamers. We employed an additional selection pressure by using RNAse to remove surface-binding aptamers and select for cell-internalizing aptamers. A common RNA sequence was identified from independent cell-SELEX procedures against two different pancreatic cancer cell lines, indicating a strong selection pressure towards this sequence from the large pool of other available sequences present in the aptamer library. The aptamer is not specific for the pancreatic cancer cell lines, and a similar sequence motif is present in previously published internalizing aptamers. The identified sequence forms a structural motif that binds to a surface protein, which either is highly abundant or has strong affinity for the selected aptamer sequence. Deselecting (removing) this sequence during cell-SELEX may increase the probability of identifying aptamers against cell type-specific targets on the cell surface. |
| format | Article |
| id | doaj-art-36d173cc028e4eb2acb8acc4a0736f5e |
| institution | Kabale University |
| issn | 2090-0201 2090-021X |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-36d173cc028e4eb2acb8acc4a0736f5e2025-08-20T03:55:01ZengWileyJournal of Nucleic Acids2090-02012090-021X2017-01-01201710.1155/2017/49430724943072Cell-SELEX Identifies a “Sticky” RNA Aptamer SequencePartha Ray0Rebekah R. White1Department of Surgery, UC San Diego School of Medicine, La Jolla, CA, USADepartment of Surgery, UC San Diego School of Medicine, La Jolla, CA, USACell-SELEX is performed to select for cell binding aptamers. We employed an additional selection pressure by using RNAse to remove surface-binding aptamers and select for cell-internalizing aptamers. A common RNA sequence was identified from independent cell-SELEX procedures against two different pancreatic cancer cell lines, indicating a strong selection pressure towards this sequence from the large pool of other available sequences present in the aptamer library. The aptamer is not specific for the pancreatic cancer cell lines, and a similar sequence motif is present in previously published internalizing aptamers. The identified sequence forms a structural motif that binds to a surface protein, which either is highly abundant or has strong affinity for the selected aptamer sequence. Deselecting (removing) this sequence during cell-SELEX may increase the probability of identifying aptamers against cell type-specific targets on the cell surface.http://dx.doi.org/10.1155/2017/4943072 |
| spellingShingle | Partha Ray Rebekah R. White Cell-SELEX Identifies a “Sticky” RNA Aptamer Sequence Journal of Nucleic Acids |
| title | Cell-SELEX Identifies a “Sticky” RNA Aptamer Sequence |
| title_full | Cell-SELEX Identifies a “Sticky” RNA Aptamer Sequence |
| title_fullStr | Cell-SELEX Identifies a “Sticky” RNA Aptamer Sequence |
| title_full_unstemmed | Cell-SELEX Identifies a “Sticky” RNA Aptamer Sequence |
| title_short | Cell-SELEX Identifies a “Sticky” RNA Aptamer Sequence |
| title_sort | cell selex identifies a sticky rna aptamer sequence |
| url | http://dx.doi.org/10.1155/2017/4943072 |
| work_keys_str_mv | AT partharay cellselexidentifiesastickyrnaaptamersequence AT rebekahrwhite cellselexidentifiesastickyrnaaptamersequence |