Immunocytochemistry and DNA-Image Cytometry in Diagnostic Effusion Cytology I. Prevalence of Markers in Tumour Cell Positive and Negative Smears
To determine the prevalence of immunocytochemical positivities for a panel of antibodies in benign and malignant cells in effusions with known follow‐up in order to use these as diagnostic markers. Besides their ability to identify malignant epithelial cells their contribution to the differential di...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1999-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1999/459158 |
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| author | Helma Motherby Mary Kube Nikolaus Friedrichs Bahram Nadjari Kristiane Knops Andreas Donner Betty Baschiera Peter Dalquen Alfred Böcking |
| author_facet | Helma Motherby Mary Kube Nikolaus Friedrichs Bahram Nadjari Kristiane Knops Andreas Donner Betty Baschiera Peter Dalquen Alfred Böcking |
| author_sort | Helma Motherby |
| collection | DOAJ |
| description | To determine the prevalence of immunocytochemical positivities for a panel of antibodies in benign and malignant cells in effusions with known follow‐up in order to use these as diagnostic markers. Besides their ability to identify malignant epithelial cells their contribution to the differential diagnosis between carcinomatoses and mesotheliomas was investigated. 101 tumour cell positive and 53 negative effusions were stained with 12 different antibodies. Results were scored semiquantitatively per cell type. Furthermore, DNA‐image cytometry was performed. While prevalence of Ber‐EP4 positivity was 95.4% in metastatic carcinoma cells, it was 0% in those from mesotheliomas. No cell type reacted with this marker in benign effusions (0%). Ber‐EP4 correctly differentiated between metastatic carcinoma and mesothelioma in 98.0%. Prevalence of DNA‐aneuploidy was 95.4% in metastatic carcinomas, 57.1% in mesotheliomas and 0% in reactive effusions. Combining immunocytochemistry (Ber‐EP4 positivity) and DNA‐image cytometry (aneuploidy) results in a 100% detection of metastatic carcinomatoses and 57.1% of mesotheliomas. Both markers furthermore allowed a correct differentiation of these entities in 98%. |
| format | Article |
| id | doaj-art-36cd47c0390e46ccae2d39dbf53f8329 |
| institution | OA Journals |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1999-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-36cd47c0390e46ccae2d39dbf53f83292025-08-20T02:07:41ZengWileyAnalytical Cellular Pathology0921-89121878-36511999-01-0119172010.1155/1999/459158Immunocytochemistry and DNA-Image Cytometry in Diagnostic Effusion Cytology I. Prevalence of Markers in Tumour Cell Positive and Negative SmearsHelma Motherby0Mary Kube1Nikolaus Friedrichs2Bahram Nadjari3Kristiane Knops4Andreas Donner5Betty Baschiera6Peter Dalquen7Alfred Böcking8Institute of Cytopathology, Heinrich Heine University, Moorenstr. 5, D‐40225 Düsseldorf, GermanyInstitute of Cytopathology, Heinrich Heine University, Moorenstr. 5, D‐40225 Düsseldorf, GermanyInstitute of Cytopathology, Heinrich Heine University, Moorenstr. 5, D‐40225 Düsseldorf, GermanyInstitute of Cytopathology, Heinrich Heine University, Moorenstr. 5, D‐40225 Düsseldorf, GermanyInstitute of Cytopathology, Heinrich Heine University, Moorenstr. 5, D‐40225 Düsseldorf, GermanyInstitute of Pathology, Heinrich Heine University, Moorenstr. 5, D‐40225 Düsseldorf, GermanyDivision of Cytopathology, Institute of Pathology, University Hospital Basel, Schönbeinstr. 40, CH‐4003 Basel, SwitzerlandDivision of Cytopathology, Institute of Pathology, University Hospital Basel, Schönbeinstr. 40, CH‐4003 Basel, SwitzerlandInstitute of Cytopathology, Heinrich Heine University, Moorenstr. 5, D‐40225 Düsseldorf, GermanyTo determine the prevalence of immunocytochemical positivities for a panel of antibodies in benign and malignant cells in effusions with known follow‐up in order to use these as diagnostic markers. Besides their ability to identify malignant epithelial cells their contribution to the differential diagnosis between carcinomatoses and mesotheliomas was investigated. 101 tumour cell positive and 53 negative effusions were stained with 12 different antibodies. Results were scored semiquantitatively per cell type. Furthermore, DNA‐image cytometry was performed. While prevalence of Ber‐EP4 positivity was 95.4% in metastatic carcinoma cells, it was 0% in those from mesotheliomas. No cell type reacted with this marker in benign effusions (0%). Ber‐EP4 correctly differentiated between metastatic carcinoma and mesothelioma in 98.0%. Prevalence of DNA‐aneuploidy was 95.4% in metastatic carcinomas, 57.1% in mesotheliomas and 0% in reactive effusions. Combining immunocytochemistry (Ber‐EP4 positivity) and DNA‐image cytometry (aneuploidy) results in a 100% detection of metastatic carcinomatoses and 57.1% of mesotheliomas. Both markers furthermore allowed a correct differentiation of these entities in 98%.http://dx.doi.org/10.1155/1999/459158 |
| spellingShingle | Helma Motherby Mary Kube Nikolaus Friedrichs Bahram Nadjari Kristiane Knops Andreas Donner Betty Baschiera Peter Dalquen Alfred Böcking Immunocytochemistry and DNA-Image Cytometry in Diagnostic Effusion Cytology I. Prevalence of Markers in Tumour Cell Positive and Negative Smears Analytical Cellular Pathology |
| title | Immunocytochemistry and DNA-Image Cytometry in Diagnostic Effusion Cytology I. Prevalence of Markers in Tumour Cell Positive and Negative Smears |
| title_full | Immunocytochemistry and DNA-Image Cytometry in Diagnostic Effusion Cytology I. Prevalence of Markers in Tumour Cell Positive and Negative Smears |
| title_fullStr | Immunocytochemistry and DNA-Image Cytometry in Diagnostic Effusion Cytology I. Prevalence of Markers in Tumour Cell Positive and Negative Smears |
| title_full_unstemmed | Immunocytochemistry and DNA-Image Cytometry in Diagnostic Effusion Cytology I. Prevalence of Markers in Tumour Cell Positive and Negative Smears |
| title_short | Immunocytochemistry and DNA-Image Cytometry in Diagnostic Effusion Cytology I. Prevalence of Markers in Tumour Cell Positive and Negative Smears |
| title_sort | immunocytochemistry and dna image cytometry in diagnostic effusion cytology i prevalence of markers in tumour cell positive and negative smears |
| url | http://dx.doi.org/10.1155/1999/459158 |
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