Biomechanical and histomorphometric characterization of the melatonin treatment effect in the carotid artery subjected to hypobaric hypoxia
This study aims to assess the efficacy of melatonin in mitigating the adverse effects of hypobaric hypoxia on the cardiovascular system of neonatal lambs (30 days old). Two groups were considered for this purpose: (i) Melatonin-treated group (N = 5) and (ii) Control group (N = 6) without treatment....
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-04-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1554004/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849726103294312448 |
|---|---|
| author | Eugenio Rivera Alvaro Navarrete Claudio M. Garcia-Herrera Leonardo Gordillo Enrique Cerda Diego J. Celentano Alejandro Gonzalez-Candia Emilio A. Herrera Emilio A. Herrera |
| author_facet | Eugenio Rivera Alvaro Navarrete Claudio M. Garcia-Herrera Leonardo Gordillo Enrique Cerda Diego J. Celentano Alejandro Gonzalez-Candia Emilio A. Herrera Emilio A. Herrera |
| author_sort | Eugenio Rivera |
| collection | DOAJ |
| description | This study aims to assess the efficacy of melatonin in mitigating the adverse effects of hypobaric hypoxia on the cardiovascular system of neonatal lambs (30 days old). Two groups were considered for this purpose: (i) Melatonin-treated group (N = 5) and (ii) Control group (N = 6) without treatment. All subjects were exposed to hypobaric hypoxia during gestation and perinatal periods, with melatonin administered after birth. The study focused on the carotid artery, a known predictor of cardiovascular risk. Biomechanical tests, morphometric, and histological measurements were conducted, and a numerical model was developed based on the biomechanical data. Key findings showed remodeling effects: Firstly, a realignment of collagen fibers towards a longitudinal direction was observed with melatonin treatment, similar to non-hypoxic arteries. Second, changes in residual stress and ex-vivo luminal radius were noted, aiming to reduce wall stress and increase vascular resistance. These changes indicate an antihypertensive response, reducing the effects of increased blood pressure and flow due to hypobaric hypoxia. This study demonstrates that biomechanical and histomorphometric methodologies effectively assess the beneficial effects of melatonin treatment under hypobaric hypoxia exposure. |
| format | Article |
| id | doaj-art-36cc564c6965435fa1adb81eef338c50 |
| institution | DOAJ |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-36cc564c6965435fa1adb81eef338c502025-08-20T03:10:18ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-04-011310.3389/fbioe.2025.15540041554004Biomechanical and histomorphometric characterization of the melatonin treatment effect in the carotid artery subjected to hypobaric hypoxiaEugenio Rivera0Alvaro Navarrete1Claudio M. Garcia-Herrera2Leonardo Gordillo3Enrique Cerda4Diego J. Celentano5Alejandro Gonzalez-Candia6Emilio A. Herrera7Emilio A. Herrera8Departamento de Ingeniería Mecánica, Universidad de Santiago de Chile, Santiago, ChileDepartamento de Ingeniería Mecánica, Universidad de Santiago de Chile, Santiago, ChileDepartamento de Ingeniería Mecánica, Universidad de Santiago de Chile, Santiago, ChileDepartamento de Física, Universidad de Santiago de Chile, Santiago, ChileDepartamento de Física, Universidad de Santiago de Chile, Santiago, ChileDepartment of Mechanical and Metallurgical Engineering, Pontificia Universidad Católica de Chile, Santiago, ChileInstitute of Health Sciences, Universidad de O’Higgins, Rancagua, ChilePathophysiology Program, Faculty of Medicine, Institute of Biomedical Sciences (ICBM), Universidad de Chile, Santiago, ChileInternational Center for Andean Studies (INCAS), Universidad de Chile, Santiago, ChileThis study aims to assess the efficacy of melatonin in mitigating the adverse effects of hypobaric hypoxia on the cardiovascular system of neonatal lambs (30 days old). Two groups were considered for this purpose: (i) Melatonin-treated group (N = 5) and (ii) Control group (N = 6) without treatment. All subjects were exposed to hypobaric hypoxia during gestation and perinatal periods, with melatonin administered after birth. The study focused on the carotid artery, a known predictor of cardiovascular risk. Biomechanical tests, morphometric, and histological measurements were conducted, and a numerical model was developed based on the biomechanical data. Key findings showed remodeling effects: Firstly, a realignment of collagen fibers towards a longitudinal direction was observed with melatonin treatment, similar to non-hypoxic arteries. Second, changes in residual stress and ex-vivo luminal radius were noted, aiming to reduce wall stress and increase vascular resistance. These changes indicate an antihypertensive response, reducing the effects of increased blood pressure and flow due to hypobaric hypoxia. This study demonstrates that biomechanical and histomorphometric methodologies effectively assess the beneficial effects of melatonin treatment under hypobaric hypoxia exposure.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1554004/fullbiomechanicscarotid arteryhypobaric hypoxiamelatoninbiomechanical testsfinite element method |
| spellingShingle | Eugenio Rivera Alvaro Navarrete Claudio M. Garcia-Herrera Leonardo Gordillo Enrique Cerda Diego J. Celentano Alejandro Gonzalez-Candia Emilio A. Herrera Emilio A. Herrera Biomechanical and histomorphometric characterization of the melatonin treatment effect in the carotid artery subjected to hypobaric hypoxia Frontiers in Bioengineering and Biotechnology biomechanics carotid artery hypobaric hypoxia melatonin biomechanical tests finite element method |
| title | Biomechanical and histomorphometric characterization of the melatonin treatment effect in the carotid artery subjected to hypobaric hypoxia |
| title_full | Biomechanical and histomorphometric characterization of the melatonin treatment effect in the carotid artery subjected to hypobaric hypoxia |
| title_fullStr | Biomechanical and histomorphometric characterization of the melatonin treatment effect in the carotid artery subjected to hypobaric hypoxia |
| title_full_unstemmed | Biomechanical and histomorphometric characterization of the melatonin treatment effect in the carotid artery subjected to hypobaric hypoxia |
| title_short | Biomechanical and histomorphometric characterization of the melatonin treatment effect in the carotid artery subjected to hypobaric hypoxia |
| title_sort | biomechanical and histomorphometric characterization of the melatonin treatment effect in the carotid artery subjected to hypobaric hypoxia |
| topic | biomechanics carotid artery hypobaric hypoxia melatonin biomechanical tests finite element method |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1554004/full |
| work_keys_str_mv | AT eugeniorivera biomechanicalandhistomorphometriccharacterizationofthemelatonintreatmenteffectinthecarotidarterysubjectedtohypobarichypoxia AT alvaronavarrete biomechanicalandhistomorphometriccharacterizationofthemelatonintreatmenteffectinthecarotidarterysubjectedtohypobarichypoxia AT claudiomgarciaherrera biomechanicalandhistomorphometriccharacterizationofthemelatonintreatmenteffectinthecarotidarterysubjectedtohypobarichypoxia AT leonardogordillo biomechanicalandhistomorphometriccharacterizationofthemelatonintreatmenteffectinthecarotidarterysubjectedtohypobarichypoxia AT enriquecerda biomechanicalandhistomorphometriccharacterizationofthemelatonintreatmenteffectinthecarotidarterysubjectedtohypobarichypoxia AT diegojcelentano biomechanicalandhistomorphometriccharacterizationofthemelatonintreatmenteffectinthecarotidarterysubjectedtohypobarichypoxia AT alejandrogonzalezcandia biomechanicalandhistomorphometriccharacterizationofthemelatonintreatmenteffectinthecarotidarterysubjectedtohypobarichypoxia AT emilioaherrera biomechanicalandhistomorphometriccharacterizationofthemelatonintreatmenteffectinthecarotidarterysubjectedtohypobarichypoxia AT emilioaherrera biomechanicalandhistomorphometriccharacterizationofthemelatonintreatmenteffectinthecarotidarterysubjectedtohypobarichypoxia |