Expression of CD68+ Tumor associated macrophages in relation to β-catenin in carcinoma stomach
Background: With no unified system for tumor associated macrophages (TAMs) density assessment, limited information is available on their relationship with β-catenin expression. Aim: To evaluate the density of CD68+ TAMs in gastric adenocarcinoma samples by immunohistochemistry and correlate it with...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2023-07-01
|
| Series: | Indian Journal of Pathology and Microbiology |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/ijpm.ijpm_535_22 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832591221036417024 |
|---|---|
| author | Nikhil Sharma Ranjita Panigrahi Prita Pradhan Sabyasachi Parida Saroj R. Sahoo |
| author_facet | Nikhil Sharma Ranjita Panigrahi Prita Pradhan Sabyasachi Parida Saroj R. Sahoo |
| author_sort | Nikhil Sharma |
| collection | DOAJ |
| description | Background:
With no unified system for tumor associated macrophages (TAMs) density assessment, limited information is available on their relationship with β-catenin expression.
Aim:
To evaluate the density of CD68+ TAMs in gastric adenocarcinoma samples by immunohistochemistry and correlate it with grade, stage, invasion, and beta-catenin.
Designs and Settings:
Formalin fixed paraffin embedded (FFPE) blocks from gastrectomy specimens of proven gastric adenocarcinoma were prospectively and retrospectively were studied over a period of two years.
Materials and Methods:
Immunohistochemistry with CD68 and β-catenin was performed. TAM density was qualitatively compared in “tumor” versus “stroma” and “tumor” versus “non-tumor” regions. Quantitative CD68+ TAM density was assessed using different methods and compared. Cases were classified as high and low TAM based on the median value and correlated with histologic type, location, grade, stage and β-catenin expression pattern.
Statistical Analysis:
Spearman’s rank correlation test was used to compare the different methods of TAM density evaluation. The categorical variables were studied using Pearson’s Chi-square or Fisher’s exact test. CD68+ TAM density and β-catenin expression were correlated by analysis of variance. A P value ≤ 0.05 was taken as statistically significant.
Results:
The CD68+ TAMs in the “tumor” versus “non-tumor” area (p = 0.34) and “tumor” versus “stroma distribution” (p = 0.81) did not show any statistical significance. All methods of TAM density were found to be comparable. High TAM group is significantly associated with lymphovascular invasion, tumor depth, lymph node metastasis, and abnormal β-catenin expression.
Conclusion:
TAMs density plays an important role in the tumor stage. Macrophages may possibly induce gastric cancer invasiveness by activating β-catenin pathway. |
| format | Article |
| id | doaj-art-36ca7f80ab434db5bd9978576cd26564 |
| institution | Kabale University |
| issn | 0377-4929 0974-5130 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Pathology and Microbiology |
| spelling | doaj-art-36ca7f80ab434db5bd9978576cd265642025-01-22T15:07:46ZengWolters Kluwer Medknow PublicationsIndian Journal of Pathology and Microbiology0377-49290974-51302023-07-01671152010.4103/ijpm.ijpm_535_22Expression of CD68+ Tumor associated macrophages in relation to β-catenin in carcinoma stomachNikhil SharmaRanjita PanigrahiPrita PradhanSabyasachi ParidaSaroj R. SahooBackground: With no unified system for tumor associated macrophages (TAMs) density assessment, limited information is available on their relationship with β-catenin expression. Aim: To evaluate the density of CD68+ TAMs in gastric adenocarcinoma samples by immunohistochemistry and correlate it with grade, stage, invasion, and beta-catenin. Designs and Settings: Formalin fixed paraffin embedded (FFPE) blocks from gastrectomy specimens of proven gastric adenocarcinoma were prospectively and retrospectively were studied over a period of two years. Materials and Methods: Immunohistochemistry with CD68 and β-catenin was performed. TAM density was qualitatively compared in “tumor” versus “stroma” and “tumor” versus “non-tumor” regions. Quantitative CD68+ TAM density was assessed using different methods and compared. Cases were classified as high and low TAM based on the median value and correlated with histologic type, location, grade, stage and β-catenin expression pattern. Statistical Analysis: Spearman’s rank correlation test was used to compare the different methods of TAM density evaluation. The categorical variables were studied using Pearson’s Chi-square or Fisher’s exact test. CD68+ TAM density and β-catenin expression were correlated by analysis of variance. A P value ≤ 0.05 was taken as statistically significant. Results: The CD68+ TAMs in the “tumor” versus “non-tumor” area (p = 0.34) and “tumor” versus “stroma distribution” (p = 0.81) did not show any statistical significance. All methods of TAM density were found to be comparable. High TAM group is significantly associated with lymphovascular invasion, tumor depth, lymph node metastasis, and abnormal β-catenin expression. Conclusion: TAMs density plays an important role in the tumor stage. Macrophages may possibly induce gastric cancer invasiveness by activating β-catenin pathway.https://journals.lww.com/10.4103/ijpm.ijpm_535_22β-cateningastrectomymacrophagesmicroenvironmentstomach |
| spellingShingle | Nikhil Sharma Ranjita Panigrahi Prita Pradhan Sabyasachi Parida Saroj R. Sahoo Expression of CD68+ Tumor associated macrophages in relation to β-catenin in carcinoma stomach Indian Journal of Pathology and Microbiology β-catenin gastrectomy macrophages microenvironment stomach |
| title | Expression of CD68+ Tumor associated macrophages in relation to β-catenin in carcinoma stomach |
| title_full | Expression of CD68+ Tumor associated macrophages in relation to β-catenin in carcinoma stomach |
| title_fullStr | Expression of CD68+ Tumor associated macrophages in relation to β-catenin in carcinoma stomach |
| title_full_unstemmed | Expression of CD68+ Tumor associated macrophages in relation to β-catenin in carcinoma stomach |
| title_short | Expression of CD68+ Tumor associated macrophages in relation to β-catenin in carcinoma stomach |
| title_sort | expression of cd68 tumor associated macrophages in relation to β catenin in carcinoma stomach |
| topic | β-catenin gastrectomy macrophages microenvironment stomach |
| url | https://journals.lww.com/10.4103/ijpm.ijpm_535_22 |
| work_keys_str_mv | AT nikhilsharma expressionofcd68tumorassociatedmacrophagesinrelationtobcateninincarcinomastomach AT ranjitapanigrahi expressionofcd68tumorassociatedmacrophagesinrelationtobcateninincarcinomastomach AT pritapradhan expressionofcd68tumorassociatedmacrophagesinrelationtobcateninincarcinomastomach AT sabyasachiparida expressionofcd68tumorassociatedmacrophagesinrelationtobcateninincarcinomastomach AT sarojrsahoo expressionofcd68tumorassociatedmacrophagesinrelationtobcateninincarcinomastomach |