Detection of anti-MGL_1304 IgE using the ImmunoCAP system for diagnosis of type I allergy to sweat
Background: Type I allergy to sweat is involved in the pathogenesis of atopic dermatitis (AD) and cholinergic urticaria (CholU), with MGL_1304 from Malassezia globosa being the major causative antigen. Currently, no standard diagnostic test exists for sweat allergy that uses serum. Methods: The Immu...
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Elsevier
2025-04-01
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| Series: | Allergology International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1323893024001308 |
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| author | Shunsuke Takahagi Masaya Moriwaki Kaori Ishii Natsuko Asakura Michihiro Hide |
| author_facet | Shunsuke Takahagi Masaya Moriwaki Kaori Ishii Natsuko Asakura Michihiro Hide |
| author_sort | Shunsuke Takahagi |
| collection | DOAJ |
| description | Background: Type I allergy to sweat is involved in the pathogenesis of atopic dermatitis (AD) and cholinergic urticaria (CholU), with MGL_1304 from Malassezia globosa being the major causative antigen. Currently, no standard diagnostic test exists for sweat allergy that uses serum. Methods: The ImmunoCAP (iCAP) system to measure antigen-specific IgE was developed using recombinant MGL_1304 (rMGL_1304). Using a positive histamine release test (HRT) against the semi-purified sweat antigen (QR) as a criterion for diagnosing sweat allergy, the diagnostic usefulness of anti-MGL_1304 IgE detected through iCAP was analyzed in comparison with conventional anti-Malassezia antigen m227 IgE (anti-m227 IgE). Results: The iCAP system with rMGL_1304 detected anti-MGL_1304 IgE in serum samples without detection of non-specific reactions. In 93 patients with AD or CholU, of which 58 were HRT-positive, anti-MGL_1304 IgE titers correlated with histamine release levels in HRT against QR better than anti-m227 IgE titers. The cutoff value for sweat allergy diagnosis was 1.55 UA/mL for anti-m227 IgE (sensitivity: 79.3 %; specificity: 65.7 %) and 0.671 UA/mL for anti-MGL_1304 IgE (sensitivity: 84.5 %; specificity: 80.0 %). Clinical features of AD and CholU were partially associated with anti-m227 IgE and anti-MGL_1304 IgE titers but not with histamine release in HRT using QR. Conclusions: Anti-MGL_1304 IgE detection using iCAP is simple and can help diagnosis of sweat allergy with better accuracy than conventional anti-Malassezia antigen IgE. |
| format | Article |
| id | doaj-art-36be76dc94dc40c796e0e4c4f72b9455 |
| institution | OA Journals |
| issn | 1323-8930 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Allergology International |
| spelling | doaj-art-36be76dc94dc40c796e0e4c4f72b94552025-08-20T02:09:56ZengElsevierAllergology International1323-89302025-04-0174231632410.1016/j.alit.2024.11.004Detection of anti-MGL_1304 IgE using the ImmunoCAP system for diagnosis of type I allergy to sweatShunsuke Takahagi0Masaya Moriwaki1Kaori Ishii2Natsuko Asakura3Michihiro Hide4Department of Dermatology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan; Department of Dermatology, JA Hiroshima General Hospital, Hiroshima, JapanDepartment of Dermatology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, JapanDepartment of Dermatology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, JapanReference Laboratory, Medical Scientific Affairs, Thermo Fisher Diagnostics K.K., Tokyo, JapanDepartment of Dermatology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan; Department of Dermatology, Hiroshima City Hiroshima Citizens Hospital, Hiroshima, Japan; Corresponding author. Department of Dermatology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.Background: Type I allergy to sweat is involved in the pathogenesis of atopic dermatitis (AD) and cholinergic urticaria (CholU), with MGL_1304 from Malassezia globosa being the major causative antigen. Currently, no standard diagnostic test exists for sweat allergy that uses serum. Methods: The ImmunoCAP (iCAP) system to measure antigen-specific IgE was developed using recombinant MGL_1304 (rMGL_1304). Using a positive histamine release test (HRT) against the semi-purified sweat antigen (QR) as a criterion for diagnosing sweat allergy, the diagnostic usefulness of anti-MGL_1304 IgE detected through iCAP was analyzed in comparison with conventional anti-Malassezia antigen m227 IgE (anti-m227 IgE). Results: The iCAP system with rMGL_1304 detected anti-MGL_1304 IgE in serum samples without detection of non-specific reactions. In 93 patients with AD or CholU, of which 58 were HRT-positive, anti-MGL_1304 IgE titers correlated with histamine release levels in HRT against QR better than anti-m227 IgE titers. The cutoff value for sweat allergy diagnosis was 1.55 UA/mL for anti-m227 IgE (sensitivity: 79.3 %; specificity: 65.7 %) and 0.671 UA/mL for anti-MGL_1304 IgE (sensitivity: 84.5 %; specificity: 80.0 %). Clinical features of AD and CholU were partially associated with anti-m227 IgE and anti-MGL_1304 IgE titers but not with histamine release in HRT using QR. Conclusions: Anti-MGL_1304 IgE detection using iCAP is simple and can help diagnosis of sweat allergy with better accuracy than conventional anti-Malassezia antigen IgE.http://www.sciencedirect.com/science/article/pii/S1323893024001308Atopic dermatitisCholinergic urticariaImmunoCAPMGL_1304Sweat allergy |
| spellingShingle | Shunsuke Takahagi Masaya Moriwaki Kaori Ishii Natsuko Asakura Michihiro Hide Detection of anti-MGL_1304 IgE using the ImmunoCAP system for diagnosis of type I allergy to sweat Allergology International Atopic dermatitis Cholinergic urticaria ImmunoCAP MGL_1304 Sweat allergy |
| title | Detection of anti-MGL_1304 IgE using the ImmunoCAP system for diagnosis of type I allergy to sweat |
| title_full | Detection of anti-MGL_1304 IgE using the ImmunoCAP system for diagnosis of type I allergy to sweat |
| title_fullStr | Detection of anti-MGL_1304 IgE using the ImmunoCAP system for diagnosis of type I allergy to sweat |
| title_full_unstemmed | Detection of anti-MGL_1304 IgE using the ImmunoCAP system for diagnosis of type I allergy to sweat |
| title_short | Detection of anti-MGL_1304 IgE using the ImmunoCAP system for diagnosis of type I allergy to sweat |
| title_sort | detection of anti mgl 1304 ige using the immunocap system for diagnosis of type i allergy to sweat |
| topic | Atopic dermatitis Cholinergic urticaria ImmunoCAP MGL_1304 Sweat allergy |
| url | http://www.sciencedirect.com/science/article/pii/S1323893024001308 |
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