Chemical Fingerprint and Metabolic Profile Analysis of Tianshu Tablets by Ultra-High Performance Liquid Chromatography/Quadrupole-Time of Flight Mass Spectrometry
In recent years, the chemical fingerprinting of traditional Chinese medicines and the metabolites in these compounds has been a hot topic. In the present study, the chemical fingerprint of Tianshu tablets (TST) and the metabolic characteristics of compounds in rats after intragastric administration...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/9158942 |
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| author | Lin Chen Renhao Chen Hui Ouyang Qi Wang Zhifeng Li Yulin Feng Shilin Yang |
| author_facet | Lin Chen Renhao Chen Hui Ouyang Qi Wang Zhifeng Li Yulin Feng Shilin Yang |
| author_sort | Lin Chen |
| collection | DOAJ |
| description | In recent years, the chemical fingerprinting of traditional Chinese medicines and the metabolites in these compounds has been a hot topic. In the present study, the chemical fingerprint of Tianshu tablets (TST) and the metabolic characteristics of compounds in rats after intragastric administration were studied by ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC/Q-TOF MS). In a preliminary study, 77 chemical components in TST were determined by comparison with retention times, accurate molecular mass, and characteristic fragment ions of the known compounds in the literature and some well-known compounds were analyzed in detail, and the fragmentation pathways for parishins B, gastrodin A, and cnidilide or neocnilide were specifically analyzed. After intragastric administration of TST (4 g/kg) to rats, a total of 61 compounds were detected in plasma samples, including 7 prototypes and 54 metabolites. After further analysis, it was found that these metabolites were subjected to glucuronidation, sulfation, methylation, hydroxylation, dehydrogenation, or mixed metabolic processes. Hydroxylation and glucuronidation were finally confirmed as the main metabolic pathways. This is the first research on the chemical fingerprint and metabolites of TST, which lays a foundation for further investigation of TST. |
| format | Article |
| id | doaj-art-36b120bfa9634b33b6c2a05b233be5a7 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-36b120bfa9634b33b6c2a05b233be5a72025-02-03T07:24:12ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732019-01-01201910.1155/2019/91589429158942Chemical Fingerprint and Metabolic Profile Analysis of Tianshu Tablets by Ultra-High Performance Liquid Chromatography/Quadrupole-Time of Flight Mass SpectrometryLin Chen0Renhao Chen1Hui Ouyang2Qi Wang3Zhifeng Li4Yulin Feng5Shilin Yang6College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, ChinaCollege of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, ChinaState Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, ChinaCollege of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, ChinaCollege of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, ChinaState Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, ChinaState Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, ChinaIn recent years, the chemical fingerprinting of traditional Chinese medicines and the metabolites in these compounds has been a hot topic. In the present study, the chemical fingerprint of Tianshu tablets (TST) and the metabolic characteristics of compounds in rats after intragastric administration were studied by ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC/Q-TOF MS). In a preliminary study, 77 chemical components in TST were determined by comparison with retention times, accurate molecular mass, and characteristic fragment ions of the known compounds in the literature and some well-known compounds were analyzed in detail, and the fragmentation pathways for parishins B, gastrodin A, and cnidilide or neocnilide were specifically analyzed. After intragastric administration of TST (4 g/kg) to rats, a total of 61 compounds were detected in plasma samples, including 7 prototypes and 54 metabolites. After further analysis, it was found that these metabolites were subjected to glucuronidation, sulfation, methylation, hydroxylation, dehydrogenation, or mixed metabolic processes. Hydroxylation and glucuronidation were finally confirmed as the main metabolic pathways. This is the first research on the chemical fingerprint and metabolites of TST, which lays a foundation for further investigation of TST.http://dx.doi.org/10.1155/2019/9158942 |
| spellingShingle | Lin Chen Renhao Chen Hui Ouyang Qi Wang Zhifeng Li Yulin Feng Shilin Yang Chemical Fingerprint and Metabolic Profile Analysis of Tianshu Tablets by Ultra-High Performance Liquid Chromatography/Quadrupole-Time of Flight Mass Spectrometry Journal of Analytical Methods in Chemistry |
| title | Chemical Fingerprint and Metabolic Profile Analysis of Tianshu Tablets by Ultra-High Performance Liquid Chromatography/Quadrupole-Time of Flight Mass Spectrometry |
| title_full | Chemical Fingerprint and Metabolic Profile Analysis of Tianshu Tablets by Ultra-High Performance Liquid Chromatography/Quadrupole-Time of Flight Mass Spectrometry |
| title_fullStr | Chemical Fingerprint and Metabolic Profile Analysis of Tianshu Tablets by Ultra-High Performance Liquid Chromatography/Quadrupole-Time of Flight Mass Spectrometry |
| title_full_unstemmed | Chemical Fingerprint and Metabolic Profile Analysis of Tianshu Tablets by Ultra-High Performance Liquid Chromatography/Quadrupole-Time of Flight Mass Spectrometry |
| title_short | Chemical Fingerprint and Metabolic Profile Analysis of Tianshu Tablets by Ultra-High Performance Liquid Chromatography/Quadrupole-Time of Flight Mass Spectrometry |
| title_sort | chemical fingerprint and metabolic profile analysis of tianshu tablets by ultra high performance liquid chromatography quadrupole time of flight mass spectrometry |
| url | http://dx.doi.org/10.1155/2019/9158942 |
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