Soluble expression of nucleocapsid protein of feline coronavirus and reactivity identification of its polyclonal antibody
To prepare the specific antibodies against nucleocapsid (N) protein of feline coronavirus (FCoV), the full-length cDNA of N gene was amplified from type ⅠFCoV (ZJU1709) and subcloned into pCold TF prokaryotic expression vector. The recombinant N protein was induced by isopropyl-β-D-thiogalactopyrano...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2023-06-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.05.072 |
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