In Vitro Efficacy of the Monoterpene Linalool Isolated or Combined with the Nematophagous Fungus <i>Duddingtonia flagrans</i> in the Control of Sheep Gastrointestinal Nematodes
New alternatives for controlling resistant populations of gastrointestinal nematodes are being studied, including the use of plant compounds and biological control with nematophagous fungi. The objective of this study was to evaluate the in vitro anthelmintic effect of linalool and its association w...
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2024-12-01
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| author | Antônia Aniellen Raianne Moisés Aguiar Ana Maria Santos Lima Thais Ferreira Feitosa Wesley Lyeverton Correia Ribeiro Filippe Elias Freitas Soares Fabio Ribeiro Braga Vinícius Longo Ribeiro Vilela |
| author_facet | Antônia Aniellen Raianne Moisés Aguiar Ana Maria Santos Lima Thais Ferreira Feitosa Wesley Lyeverton Correia Ribeiro Filippe Elias Freitas Soares Fabio Ribeiro Braga Vinícius Longo Ribeiro Vilela |
| author_sort | Antônia Aniellen Raianne Moisés Aguiar |
| collection | DOAJ |
| description | New alternatives for controlling resistant populations of gastrointestinal nematodes are being studied, including the use of plant compounds and biological control with nematophagous fungi. The objective of this study was to evaluate the in vitro anthelmintic effect of linalool and its association with the fungus <i>Duddingtonia flagrans</i> (isolated AC001) in controlling gastrointestinal nematodes in sheep. The ovicidal activity of linalool was assessed via the Egg Hatch Test (EHT), and the larvicidal activity of linalool, alone and in combination with <i>D. flagrans</i> conidia, was evaluated via the Larval Motility Inhibition Test (LMIT) on infective larvae (L3). In the EHT, 100% inhibition occurred (at 1.25 and 2.5 mg/mL), with an LC50 of 0.49 mg/mL. In the LMIT, linalool alone inhibited 100% of larval motility (at 4% and 8%), with an LC50 of 0.42% or 4.2 mg/mL. In the combination of linalool with <i>D. flagrans</i>, there was a significant reduction in larvae, starting at 24 h, with 100% reduction after 14 days, thus being more effective in reducing L3 compared to the use of the fungus alone. It is concluded that linalool exhibits ovicidal and larvicidal activity, and its association with <i>D. flagrans</i> enhances the fungal predation capacity and potentiates anthelmintic efficacy. |
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| institution | Kabale University |
| issn | 2036-7481 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Microbiology Research |
| spelling | doaj-art-36afe05d260e433fa322a2bb4c4801392025-01-24T13:41:41ZengMDPI AGMicrobiology Research2036-74812024-12-01161110.3390/microbiolres16010001In Vitro Efficacy of the Monoterpene Linalool Isolated or Combined with the Nematophagous Fungus <i>Duddingtonia flagrans</i> in the Control of Sheep Gastrointestinal NematodesAntônia Aniellen Raianne Moisés Aguiar0Ana Maria Santos Lima1Thais Ferreira Feitosa2Wesley Lyeverton Correia Ribeiro3Filippe Elias Freitas Soares4Fabio Ribeiro Braga5Vinícius Longo Ribeiro Vilela6Postgraduate Program in Science and Animal Health, Federal University of Campina Grande, Patos 58708-110, PB, BrazilDepartment of Veterinary Medicine, Federal Institute of Paraíba, Sousa 58814-000, PB, BrazilDepartment of Veterinary Medicine, Federal Institute of Paraíba, Sousa 58814-000, PB, BrazilDepartment of Physiology and Pharmacology, Federal University of Ceará, Fortaleza 60020-181, CE, BrazilDepartment of Chemistry, Federal University of Lavras, Lavras 37200-900, MG, BrazilExperimental Parasitology and Biological Control Laboratory, University Vila-Velha, Vila Velha 29102-920, ES, BrazilPostgraduate Program in Science and Animal Health, Federal University of Campina Grande, Patos 58708-110, PB, BrazilNew alternatives for controlling resistant populations of gastrointestinal nematodes are being studied, including the use of plant compounds and biological control with nematophagous fungi. The objective of this study was to evaluate the in vitro anthelmintic effect of linalool and its association with the fungus <i>Duddingtonia flagrans</i> (isolated AC001) in controlling gastrointestinal nematodes in sheep. The ovicidal activity of linalool was assessed via the Egg Hatch Test (EHT), and the larvicidal activity of linalool, alone and in combination with <i>D. flagrans</i> conidia, was evaluated via the Larval Motility Inhibition Test (LMIT) on infective larvae (L3). In the EHT, 100% inhibition occurred (at 1.25 and 2.5 mg/mL), with an LC50 of 0.49 mg/mL. In the LMIT, linalool alone inhibited 100% of larval motility (at 4% and 8%), with an LC50 of 0.42% or 4.2 mg/mL. In the combination of linalool with <i>D. flagrans</i>, there was a significant reduction in larvae, starting at 24 h, with 100% reduction after 14 days, thus being more effective in reducing L3 compared to the use of the fungus alone. It is concluded that linalool exhibits ovicidal and larvicidal activity, and its association with <i>D. flagrans</i> enhances the fungal predation capacity and potentiates anthelmintic efficacy.https://www.mdpi.com/2036-7481/16/1/1helminthsanthelmintic resistancephytotherapymonoterpenebiological controlnematophagous fungi |
| spellingShingle | Antônia Aniellen Raianne Moisés Aguiar Ana Maria Santos Lima Thais Ferreira Feitosa Wesley Lyeverton Correia Ribeiro Filippe Elias Freitas Soares Fabio Ribeiro Braga Vinícius Longo Ribeiro Vilela In Vitro Efficacy of the Monoterpene Linalool Isolated or Combined with the Nematophagous Fungus <i>Duddingtonia flagrans</i> in the Control of Sheep Gastrointestinal Nematodes Microbiology Research helminths anthelmintic resistance phytotherapy monoterpene biological control nematophagous fungi |
| title | In Vitro Efficacy of the Monoterpene Linalool Isolated or Combined with the Nematophagous Fungus <i>Duddingtonia flagrans</i> in the Control of Sheep Gastrointestinal Nematodes |
| title_full | In Vitro Efficacy of the Monoterpene Linalool Isolated or Combined with the Nematophagous Fungus <i>Duddingtonia flagrans</i> in the Control of Sheep Gastrointestinal Nematodes |
| title_fullStr | In Vitro Efficacy of the Monoterpene Linalool Isolated or Combined with the Nematophagous Fungus <i>Duddingtonia flagrans</i> in the Control of Sheep Gastrointestinal Nematodes |
| title_full_unstemmed | In Vitro Efficacy of the Monoterpene Linalool Isolated or Combined with the Nematophagous Fungus <i>Duddingtonia flagrans</i> in the Control of Sheep Gastrointestinal Nematodes |
| title_short | In Vitro Efficacy of the Monoterpene Linalool Isolated or Combined with the Nematophagous Fungus <i>Duddingtonia flagrans</i> in the Control of Sheep Gastrointestinal Nematodes |
| title_sort | in vitro efficacy of the monoterpene linalool isolated or combined with the nematophagous fungus i duddingtonia flagrans i in the control of sheep gastrointestinal nematodes |
| topic | helminths anthelmintic resistance phytotherapy monoterpene biological control nematophagous fungi |
| url | https://www.mdpi.com/2036-7481/16/1/1 |
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