Optimization of culture conditions of microalgae in an algal-bacterial system by response surface method

An algal-bacterial system was constructed to explore the effects of culture conditions on the growth of microalgae. On the basis of the results of single factor test, three factors including the agitating rate, the initial inoculation ratio of microalgae and bacteria, and the organic load of the was...

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Main Authors: LUO Longzao, ZENG Fanjian, TIAN Guangming
Format: Article
Language:English
Published: Zhejiang University Press 2019-02-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.02.091
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author LUO Longzao
ZENG Fanjian
TIAN Guangming
author_facet LUO Longzao
ZENG Fanjian
TIAN Guangming
author_sort LUO Longzao
collection DOAJ
description An algal-bacterial system was constructed to explore the effects of culture conditions on the growth of microalgae. On the basis of the results of single factor test, three factors including the agitating rate, the initial inoculation ratio of microalgae and bacteria, and the organic load of the wastewater were optimizated by using the response surface method. The results showed that the microalgal growth was significantly affected by airtight condition, agitation rate, initial inoculation ratio and organic load of the culture system, and the microalgae grew better in the open culture system. The optimum condition by the response surface optimization was as follows: agitating rate of 1 574.29 r/min, inoculation ratio 150∶1 of bacteria-alga and organic load (chemical oxygen demand, COD) of 3 676.02 mg/L. After seven days of culture under the optimimum condition, the biomass of microalgae was 5.68 g/L, which was in accordance with the theoretically predicted value (5.69 g/L). The above results can lay a scientific basis for improving the resource recovery efficiency from wastewater by algal-bacterial system.
format Article
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institution Kabale University
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2097-5155
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publishDate 2019-02-01
publisher Zhejiang University Press
record_format Article
series 浙江大学学报. 农业与生命科学版
spelling doaj-art-369e791f538b4f65b09b7109082b606e2025-08-20T03:58:14ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552019-02-01459510110.3785/j.issn.1008-9209.2018.02.09110089209Optimization of culture conditions of microalgae in an algal-bacterial system by response surface methodLUO LongzaoZENG FanjianTIAN GuangmingAn algal-bacterial system was constructed to explore the effects of culture conditions on the growth of microalgae. On the basis of the results of single factor test, three factors including the agitating rate, the initial inoculation ratio of microalgae and bacteria, and the organic load of the wastewater were optimizated by using the response surface method. The results showed that the microalgal growth was significantly affected by airtight condition, agitation rate, initial inoculation ratio and organic load of the culture system, and the microalgae grew better in the open culture system. The optimum condition by the response surface optimization was as follows: agitating rate of 1 574.29 r/min, inoculation ratio 150∶1 of bacteria-alga and organic load (chemical oxygen demand, COD) of 3 676.02 mg/L. After seven days of culture under the optimimum condition, the biomass of microalgae was 5.68 g/L, which was in accordance with the theoretically predicted value (5.69 g/L). The above results can lay a scientific basis for improving the resource recovery efficiency from wastewater by algal-bacterial system.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.02.091microalgaealgal-bacterial systemculture conditionresponse surface methodoptimization
spellingShingle LUO Longzao
ZENG Fanjian
TIAN Guangming
Optimization of culture conditions of microalgae in an algal-bacterial system by response surface method
浙江大学学报. 农业与生命科学版
microalgae
algal-bacterial system
culture condition
response surface method
optimization
title Optimization of culture conditions of microalgae in an algal-bacterial system by response surface method
title_full Optimization of culture conditions of microalgae in an algal-bacterial system by response surface method
title_fullStr Optimization of culture conditions of microalgae in an algal-bacterial system by response surface method
title_full_unstemmed Optimization of culture conditions of microalgae in an algal-bacterial system by response surface method
title_short Optimization of culture conditions of microalgae in an algal-bacterial system by response surface method
title_sort optimization of culture conditions of microalgae in an algal bacterial system by response surface method
topic microalgae
algal-bacterial system
culture condition
response surface method
optimization
url https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.02.091
work_keys_str_mv AT luolongzao optimizationofcultureconditionsofmicroalgaeinanalgalbacterialsystembyresponsesurfacemethod
AT zengfanjian optimizationofcultureconditionsofmicroalgaeinanalgalbacterialsystembyresponsesurfacemethod
AT tianguangming optimizationofcultureconditionsofmicroalgaeinanalgalbacterialsystembyresponsesurfacemethod