Determination of the volatile fatty acid contents in rumen fluid of ruminants by headspace-gas chromatograph method
A headspace-gas chromatograph (HS-GC) (external standard method) was established for the determination of the volatile fatty acid (VFA) contents in rumen fluid of ruminants. Under the optimized headspace sampling conditions (balance temperature at 85 ℃ and equilibrium time of 30 min), the method was...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2021-10-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2020.11.201 |
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| Summary: | A headspace-gas chromatograph (HS-GC) (external standard method) was established for the determination of the volatile fatty acid (VFA) contents in rumen fluid of ruminants. Under the optimized headspace sampling conditions (balance temperature at 85 ℃ and equilibrium time of 30 min), the method was verified and used to detect the VFA. The coefficient of determination of regression equation for each compound was not less than 0.999 7. The limit of detection (LOD) was 0.036-0.329 mmol/L. The recovery rates of three different concentrations of standards were 93.540%-108.130%. The detection results showed that the relative standard deviations (RSDs) of VFA in all samples were less than 2.3%, and the method was stable within three days. The developed method is simple, which has accurate quantity and good repeatability, and suitable for the quantitative analysis of VFA in rumen fluid of ruminants. |
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| ISSN: | 1008-9209 2097-5155 |