Glucosylceramides containing very long-acyl-chain fatty acid are critical for cotton fiber elongation by influencing brassinosteroid synthesis and signaling
Sphingolipids are not only a pivotal component of membranes but also act as bioactive molecules. Cotton fiber is one of the longest plant cells and sphingolipids are closely associated with the development of cotton fiber cells. However, their function in cotton fiber cell development and its action...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2025-08-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514125001588 |
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| author | Qiaoling Wang Xingying Yan Qi Niu Hongju Zhang Huidan Tian Zheng Chen Fan Xu Yu You Yinghui Gao Zhiqing Qiao Ming Luo |
| author_facet | Qiaoling Wang Xingying Yan Qi Niu Hongju Zhang Huidan Tian Zheng Chen Fan Xu Yu You Yinghui Gao Zhiqing Qiao Ming Luo |
| author_sort | Qiaoling Wang |
| collection | DOAJ |
| description | Sphingolipids are not only a pivotal component of membranes but also act as bioactive molecules. Cotton fiber is one of the longest plant cells and sphingolipids are closely associated with the development of cotton fiber cells. However, their function in cotton fiber cell development and its action mechanism is unclear. Through cotton genetic transformation and chemistry biological approach, we identified the function and action mechanism of the glucosylceramide synthase gene GhGCS1 and its product glucosylceramide (GluCer) in cotton fiber growth. GhGCS1 was preferentially expressed at the stage of fiber elongation and localized in the endoplasmic reticulum. Overexpression of GhGCS1 promoted GluCer synthesis and fiber elongation, which was consistent with the exogenous application of GluCer (FA-C22) (containing very long-acyl-chain fatty acid) to cotton fiber in ovule culture system in vitro. Contrarily, suppressing GhGCS1 expression inhibited GluCer synthesis and fiber elongation, which was similar as the exogenous application of GluCer synthesis inhibitor, PDMP. Transcriptome analysis revealed that the fiber elongation regulated by GhGCS1 was associated with brassinosteroid (BR) synthesis and signaling related gene expression. Meanwhile, we detected the BL content of control and transgenic fiber cells. The BL content significantly increased and decreased in up- and down-regulated transgenic fibers when compared with control fibers, respectively. Furthermore, we found that PDMP treatment blocked BR synthesis and signal transduction, while exogenous application of GluCer could enhance BR synthesis and signaling. Overall, our results revealed that GhGCS1 and GluCer regulated cotton fiber elongation by influencing BR synthesis and signaling. Our study shed a novel insight on regulatory mechanism of cotton fiber elongation and provides theoretical support, genetic resources and novel transgenic materials for improvement of crop quality. |
| format | Article |
| id | doaj-art-368f7a3f15dd4f798312e18dca4d53c3 |
| institution | Kabale University |
| issn | 2214-5141 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj-art-368f7a3f15dd4f798312e18dca4d53c32025-08-22T04:56:37ZengKeAi Communications Co., Ltd.Crop Journal2214-51412025-08-011341081109210.1016/j.cj.2025.06.012Glucosylceramides containing very long-acyl-chain fatty acid are critical for cotton fiber elongation by influencing brassinosteroid synthesis and signalingQiaoling Wang0Xingying Yan1Qi Niu2Hongju Zhang3Huidan Tian4Zheng Chen5Fan Xu6Yu You7Yinghui Gao8Zhiqing Qiao9Ming Luo10College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400715, China; Corresponding author.Sphingolipids are not only a pivotal component of membranes but also act as bioactive molecules. Cotton fiber is one of the longest plant cells and sphingolipids are closely associated with the development of cotton fiber cells. However, their function in cotton fiber cell development and its action mechanism is unclear. Through cotton genetic transformation and chemistry biological approach, we identified the function and action mechanism of the glucosylceramide synthase gene GhGCS1 and its product glucosylceramide (GluCer) in cotton fiber growth. GhGCS1 was preferentially expressed at the stage of fiber elongation and localized in the endoplasmic reticulum. Overexpression of GhGCS1 promoted GluCer synthesis and fiber elongation, which was consistent with the exogenous application of GluCer (FA-C22) (containing very long-acyl-chain fatty acid) to cotton fiber in ovule culture system in vitro. Contrarily, suppressing GhGCS1 expression inhibited GluCer synthesis and fiber elongation, which was similar as the exogenous application of GluCer synthesis inhibitor, PDMP. Transcriptome analysis revealed that the fiber elongation regulated by GhGCS1 was associated with brassinosteroid (BR) synthesis and signaling related gene expression. Meanwhile, we detected the BL content of control and transgenic fiber cells. The BL content significantly increased and decreased in up- and down-regulated transgenic fibers when compared with control fibers, respectively. Furthermore, we found that PDMP treatment blocked BR synthesis and signal transduction, while exogenous application of GluCer could enhance BR synthesis and signaling. Overall, our results revealed that GhGCS1 and GluCer regulated cotton fiber elongation by influencing BR synthesis and signaling. Our study shed a novel insight on regulatory mechanism of cotton fiber elongation and provides theoretical support, genetic resources and novel transgenic materials for improvement of crop quality.http://www.sciencedirect.com/science/article/pii/S2214514125001588GhGCS1SphingolipidsGluCerPDMPBrassinosteroids |
| spellingShingle | Qiaoling Wang Xingying Yan Qi Niu Hongju Zhang Huidan Tian Zheng Chen Fan Xu Yu You Yinghui Gao Zhiqing Qiao Ming Luo Glucosylceramides containing very long-acyl-chain fatty acid are critical for cotton fiber elongation by influencing brassinosteroid synthesis and signaling Crop Journal GhGCS1 Sphingolipids GluCer PDMP Brassinosteroids |
| title | Glucosylceramides containing very long-acyl-chain fatty acid are critical for cotton fiber elongation by influencing brassinosteroid synthesis and signaling |
| title_full | Glucosylceramides containing very long-acyl-chain fatty acid are critical for cotton fiber elongation by influencing brassinosteroid synthesis and signaling |
| title_fullStr | Glucosylceramides containing very long-acyl-chain fatty acid are critical for cotton fiber elongation by influencing brassinosteroid synthesis and signaling |
| title_full_unstemmed | Glucosylceramides containing very long-acyl-chain fatty acid are critical for cotton fiber elongation by influencing brassinosteroid synthesis and signaling |
| title_short | Glucosylceramides containing very long-acyl-chain fatty acid are critical for cotton fiber elongation by influencing brassinosteroid synthesis and signaling |
| title_sort | glucosylceramides containing very long acyl chain fatty acid are critical for cotton fiber elongation by influencing brassinosteroid synthesis and signaling |
| topic | GhGCS1 Sphingolipids GluCer PDMP Brassinosteroids |
| url | http://www.sciencedirect.com/science/article/pii/S2214514125001588 |
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