Development of a super-hydrophilic anaerobic tube for the optimization of platelet-rich fibrin
Horizontal platelet-rich fibrin (H-PRF) contains a variety of bioactive growth factors and cytokines that play a key role in the process of tissue healing and regeneration. The blood collection tubes used to produce Solid-PRF (plasmatrix (PM) tubes) have previously been shown to have a great impact...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Platelets |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/09537104.2024.2316745 |
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| author | Yan Wei Yihong Cheng Hongjiang Wei Yulan Wang Xiaoxin Zhang Richard J. Miron Yufeng Zhang Shanglan Qing |
| author_facet | Yan Wei Yihong Cheng Hongjiang Wei Yulan Wang Xiaoxin Zhang Richard J. Miron Yufeng Zhang Shanglan Qing |
| author_sort | Yan Wei |
| collection | DOAJ |
| description | Horizontal platelet-rich fibrin (H-PRF) contains a variety of bioactive growth factors and cytokines that play a key role in the process of tissue healing and regeneration. The blood collection tubes used to produce Solid-PRF (plasmatrix (PM) tubes) have previously been shown to have a great impact on the morphology, strength and composition of the final H-PRF clot. Therefore, modification to PM tubes is an important step toward the future optimization of PRF. To this end, we innovatively modified the inner wall surface of the PM tubes with plasma and adjusted the gas environment inside the PM tubes to prepare super-hydrophilic anaerobic plasmatrix tubes (SHAP tubes). It was made anaerobic for the preparation of H-PRF with the aim of improving mechanical strength and bioactivity. The findings demonstrated that an anaerobic environment stimulated platelet activation within the PRF tubes. After compression, the prepared H-PRF membrane formed a fibrous cross-linked network with high fracture strength, ideal degradation characteristics, in addition to a significant increase in size. Thereafter, the H-PRF membranes prepared by the SHAP tubes significantly promoted collagen synthesis of gingival fibroblast and the mineralization of osteoblasts while maintaining excellent biocompatibility, and advantageous antibacterial properties. In conclusion, the newly modified PRF tubes had better platelet activation properties leading to better mechanical strength, a longer degradation period, and better regenerative properties in oral cell types including gingival fibroblast and alveolar osteoblasts. It also improves the success rate of H-PRF preparation in patients with coagulation dysfunction and expands the clinical application scenario. |
| format | Article |
| id | doaj-art-368e9e60247d4473bcf63d05eae852e8 |
| institution | OA Journals |
| issn | 0953-7104 1369-1635 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj-art-368e9e60247d4473bcf63d05eae852e82025-08-20T02:27:18ZengTaylor & Francis GroupPlatelets0953-71041369-16352024-12-0135110.1080/09537104.2024.2316745Development of a super-hydrophilic anaerobic tube for the optimization of platelet-rich fibrinYan Wei0Yihong Cheng1Hongjiang Wei2Yulan Wang3Xiaoxin Zhang4Richard J. Miron5Yufeng Zhang6Shanglan Qing7The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, ChinaDepartment of Periodontology, University of Bern, Bern SwitzerlandDepartment of Dental Implantology, School and Hospital of Stomatology University of Wuhan, Wuhan, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, ChinaDepartment of Stomatology Chongqing General Hospital, Chongqing, ChinaHorizontal platelet-rich fibrin (H-PRF) contains a variety of bioactive growth factors and cytokines that play a key role in the process of tissue healing and regeneration. The blood collection tubes used to produce Solid-PRF (plasmatrix (PM) tubes) have previously been shown to have a great impact on the morphology, strength and composition of the final H-PRF clot. Therefore, modification to PM tubes is an important step toward the future optimization of PRF. To this end, we innovatively modified the inner wall surface of the PM tubes with plasma and adjusted the gas environment inside the PM tubes to prepare super-hydrophilic anaerobic plasmatrix tubes (SHAP tubes). It was made anaerobic for the preparation of H-PRF with the aim of improving mechanical strength and bioactivity. The findings demonstrated that an anaerobic environment stimulated platelet activation within the PRF tubes. After compression, the prepared H-PRF membrane formed a fibrous cross-linked network with high fracture strength, ideal degradation characteristics, in addition to a significant increase in size. Thereafter, the H-PRF membranes prepared by the SHAP tubes significantly promoted collagen synthesis of gingival fibroblast and the mineralization of osteoblasts while maintaining excellent biocompatibility, and advantageous antibacterial properties. In conclusion, the newly modified PRF tubes had better platelet activation properties leading to better mechanical strength, a longer degradation period, and better regenerative properties in oral cell types including gingival fibroblast and alveolar osteoblasts. It also improves the success rate of H-PRF preparation in patients with coagulation dysfunction and expands the clinical application scenario.https://www.tandfonline.com/doi/10.1080/09537104.2024.2316745Bio-PRFH-PRFhorizontal platelet rich fibrinplasmatrixplatelet rich fibrin |
| spellingShingle | Yan Wei Yihong Cheng Hongjiang Wei Yulan Wang Xiaoxin Zhang Richard J. Miron Yufeng Zhang Shanglan Qing Development of a super-hydrophilic anaerobic tube for the optimization of platelet-rich fibrin Platelets Bio-PRF H-PRF horizontal platelet rich fibrin plasmatrix platelet rich fibrin |
| title | Development of a super-hydrophilic anaerobic tube for the optimization of platelet-rich fibrin |
| title_full | Development of a super-hydrophilic anaerobic tube for the optimization of platelet-rich fibrin |
| title_fullStr | Development of a super-hydrophilic anaerobic tube for the optimization of platelet-rich fibrin |
| title_full_unstemmed | Development of a super-hydrophilic anaerobic tube for the optimization of platelet-rich fibrin |
| title_short | Development of a super-hydrophilic anaerobic tube for the optimization of platelet-rich fibrin |
| title_sort | development of a super hydrophilic anaerobic tube for the optimization of platelet rich fibrin |
| topic | Bio-PRF H-PRF horizontal platelet rich fibrin plasmatrix platelet rich fibrin |
| url | https://www.tandfonline.com/doi/10.1080/09537104.2024.2316745 |
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