Total and Bioaccessible contents of Microelements in Multivitamin Formulations Exposed to Consumption in Brazil using in vitro Gastrointestinal Digestion (INFOGEST Protocol)
Background: Multivitamin formulations (MFs) are used worldwide to meet needs in diets for the purpose of improving health, when necessary. Therefore, evaluating the in vitro micronutrient bioaccessibility by gastrointestinal digestion methods is essential to understand the available fraction of thes...
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Elsevier
2025-06-01
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| Series: | Journal of Trace Elements and Minerals |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773050625000151 |
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| author | Abdon Luiz Ornelas Latif Thaís Luz de Sousa Laura Beatriz Souza e Souza Ivana Ferreira Simões Alex Sander Lopes da Silva Daniele Cristina Muniz Batista Santos Aníbal de Freitas Santos Júnior Clícia Maria de Jesus Benevides |
| author_facet | Abdon Luiz Ornelas Latif Thaís Luz de Sousa Laura Beatriz Souza e Souza Ivana Ferreira Simões Alex Sander Lopes da Silva Daniele Cristina Muniz Batista Santos Aníbal de Freitas Santos Júnior Clícia Maria de Jesus Benevides |
| author_sort | Abdon Luiz Ornelas Latif |
| collection | DOAJ |
| description | Background: Multivitamin formulations (MFs) are used worldwide to meet needs in diets for the purpose of improving health, when necessary. Therefore, evaluating the in vitro micronutrient bioaccessibility by gastrointestinal digestion methods is essential to understand the available fraction of these analytes in the body. Objective: In this study, methods were optimized and validated using the INFOGEST 2.0 protocol for in vitro gastrointestinal digestion and ICP OES to quantify concentrations of 13 trace elements (Al, Ba, Ca, Cu, Fe, Mn, Mo, Se, Sn, Sr, Ti, V, and Zn) in Brazilian MFs. Additionally, it seeks to correlate the levels of elements indicated on the label with the recommended daily values (RDV). Methods: Approximately, 0.75 g of MF sample were digested in a closed digestion block using 2.0 mL of 65 %(m/m) HNO3 + 5.0 mL of ultrapure water + 1.0 mL of 30 %(m/m) H2O2, at 180 °C for 90 min. Microelement bioaccessibility in Brazilian MFs through in vitro gastrointestinal digestion INFOGEST 2.0 and analysis by ICP OES were performed. Results: The range of microelements total contents (µg g-1) in the multivitamin formulations were: Al (11.2 ± 0.2 to 256.0 ± 22.2), Ba (< 0.004 to 1.2 ± 0.1), Cu (11.0 ± 0.9 to 1327.9 ± 25.6), Fe (1373.2 ± 49.7 to 8480.6 ± 146.9), Mn (443.6 ± 3.9 to 2670.7 ± 137.5), Mo (< 0.02 to 29.0 ± 1.4), Se (< 0.01 to 54.7 ± 1.6), Sn (< 0.01 to 15.0 ± 2.4), Sr (< 0.002 to 60.4 ± 0.7), Ti (< 3.0 to 99.3 ± 5.3), and Zn (551.5 ± 21.2 to 3675.5 ± 40.3). Vanadium was below the quantification limit (< 0.02). The results revealed low bioaccessible contents (%) of Cu (0 to 23.8), Fe (0 to 15.7), Mn (0 to 11.4), and Zn (0 to 9.7), emphasizing the importance of regulation and quality control for consumer confidence. Conclusion: The analysis of Brazilian MFs raised significant concerns, as most samples did not reach 50 % of the total declared content. The low mineral release during digestion simulation indicates the need for more effective formulations. |
| format | Article |
| id | doaj-art-36823e2a065d47e2aa9e77e2cf89bf1e |
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| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | Journal of Trace Elements and Minerals |
| spelling | doaj-art-36823e2a065d47e2aa9e77e2cf89bf1e2025-08-20T01:49:44ZengElsevierJournal of Trace Elements and Minerals2773-05062025-06-011210022410.1016/j.jtemin.2025.100224Total and Bioaccessible contents of Microelements in Multivitamin Formulations Exposed to Consumption in Brazil using in vitro Gastrointestinal Digestion (INFOGEST Protocol)Abdon Luiz Ornelas Latif0Thaís Luz de Sousa1Laura Beatriz Souza e Souza2Ivana Ferreira Simões3Alex Sander Lopes da Silva4Daniele Cristina Muniz Batista Santos5Aníbal de Freitas Santos Júnior6Clícia Maria de Jesus Benevides7Department of Life Sciences, Universidade do Estado da Bahia, 41195-001 Bahia, Salvador, BrazilChemistry Institute, Universidade Federal da Bahia, 40170-115 Bahia, Salvador, BrazilDepartment of Life Sciences, Universidade do Estado da Bahia, 41195-001 Bahia, Salvador, BrazilDepartment of Life Sciences, Universidade do Estado da Bahia, 41195-001 Bahia, Salvador, BrazilDepartment of Life Sciences, Universidade do Estado da Bahia, 41195-001 Bahia, Salvador, BrazilChemistry Institute, Universidade Federal da Bahia, 40170-115 Bahia, Salvador, BrazilDepartment of Life Sciences, Universidade do Estado da Bahia, 41195-001 Bahia, Salvador, Brazil; Corresponding author.Department of Life Sciences, Universidade do Estado da Bahia, 41195-001 Bahia, Salvador, BrazilBackground: Multivitamin formulations (MFs) are used worldwide to meet needs in diets for the purpose of improving health, when necessary. Therefore, evaluating the in vitro micronutrient bioaccessibility by gastrointestinal digestion methods is essential to understand the available fraction of these analytes in the body. Objective: In this study, methods were optimized and validated using the INFOGEST 2.0 protocol for in vitro gastrointestinal digestion and ICP OES to quantify concentrations of 13 trace elements (Al, Ba, Ca, Cu, Fe, Mn, Mo, Se, Sn, Sr, Ti, V, and Zn) in Brazilian MFs. Additionally, it seeks to correlate the levels of elements indicated on the label with the recommended daily values (RDV). Methods: Approximately, 0.75 g of MF sample were digested in a closed digestion block using 2.0 mL of 65 %(m/m) HNO3 + 5.0 mL of ultrapure water + 1.0 mL of 30 %(m/m) H2O2, at 180 °C for 90 min. Microelement bioaccessibility in Brazilian MFs through in vitro gastrointestinal digestion INFOGEST 2.0 and analysis by ICP OES were performed. Results: The range of microelements total contents (µg g-1) in the multivitamin formulations were: Al (11.2 ± 0.2 to 256.0 ± 22.2), Ba (< 0.004 to 1.2 ± 0.1), Cu (11.0 ± 0.9 to 1327.9 ± 25.6), Fe (1373.2 ± 49.7 to 8480.6 ± 146.9), Mn (443.6 ± 3.9 to 2670.7 ± 137.5), Mo (< 0.02 to 29.0 ± 1.4), Se (< 0.01 to 54.7 ± 1.6), Sn (< 0.01 to 15.0 ± 2.4), Sr (< 0.002 to 60.4 ± 0.7), Ti (< 3.0 to 99.3 ± 5.3), and Zn (551.5 ± 21.2 to 3675.5 ± 40.3). Vanadium was below the quantification limit (< 0.02). The results revealed low bioaccessible contents (%) of Cu (0 to 23.8), Fe (0 to 15.7), Mn (0 to 11.4), and Zn (0 to 9.7), emphasizing the importance of regulation and quality control for consumer confidence. Conclusion: The analysis of Brazilian MFs raised significant concerns, as most samples did not reach 50 % of the total declared content. The low mineral release during digestion simulation indicates the need for more effective formulations.http://www.sciencedirect.com/science/article/pii/S2773050625000151In vitro bioaccessibility, Multivitamin formulations, Trace elementsICP OES, Quality control |
| spellingShingle | Abdon Luiz Ornelas Latif Thaís Luz de Sousa Laura Beatriz Souza e Souza Ivana Ferreira Simões Alex Sander Lopes da Silva Daniele Cristina Muniz Batista Santos Aníbal de Freitas Santos Júnior Clícia Maria de Jesus Benevides Total and Bioaccessible contents of Microelements in Multivitamin Formulations Exposed to Consumption in Brazil using in vitro Gastrointestinal Digestion (INFOGEST Protocol) Journal of Trace Elements and Minerals In vitro bioaccessibility, Multivitamin formulations, Trace elements ICP OES, Quality control |
| title | Total and Bioaccessible contents of Microelements in Multivitamin Formulations Exposed to Consumption in Brazil using in vitro Gastrointestinal Digestion (INFOGEST Protocol) |
| title_full | Total and Bioaccessible contents of Microelements in Multivitamin Formulations Exposed to Consumption in Brazil using in vitro Gastrointestinal Digestion (INFOGEST Protocol) |
| title_fullStr | Total and Bioaccessible contents of Microelements in Multivitamin Formulations Exposed to Consumption in Brazil using in vitro Gastrointestinal Digestion (INFOGEST Protocol) |
| title_full_unstemmed | Total and Bioaccessible contents of Microelements in Multivitamin Formulations Exposed to Consumption in Brazil using in vitro Gastrointestinal Digestion (INFOGEST Protocol) |
| title_short | Total and Bioaccessible contents of Microelements in Multivitamin Formulations Exposed to Consumption in Brazil using in vitro Gastrointestinal Digestion (INFOGEST Protocol) |
| title_sort | total and bioaccessible contents of microelements in multivitamin formulations exposed to consumption in brazil using in vitro gastrointestinal digestion infogest protocol |
| topic | In vitro bioaccessibility, Multivitamin formulations, Trace elements ICP OES, Quality control |
| url | http://www.sciencedirect.com/science/article/pii/S2773050625000151 |
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