In Vitro Study on the Antioxidant Potentials of the Leaves and Fruits of Nauclea latifolia
This study was carried out to investigate the in vitro antioxidant potentials of the leaves and fruits of Nauclea latifolia, a straggling shrub or small tree, native to tropical Africa and Asia. Hot water extracts of the leaves and fruits of Nauclea latifolia were assessed for their total polyphenol...
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/437081 |
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| author | Ademola O. Ayeleso Oluwafemi O. Oguntibeju Nicole L. Brooks |
| author_facet | Ademola O. Ayeleso Oluwafemi O. Oguntibeju Nicole L. Brooks |
| author_sort | Ademola O. Ayeleso |
| collection | DOAJ |
| description | This study was carried out to investigate the in vitro antioxidant potentials of the leaves and fruits of
Nauclea latifolia, a straggling shrub or small tree, native to tropical Africa and Asia. Hot water extracts of the leaves and fruits of Nauclea latifolia were assessed for their total polyphenolic, flavanol, and flavonol contents as well as 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability, ferric reducing antioxidant power (FRAP), Trolox equivalence antioxidant capacity (TEAC), and oxygen radical absorbance capacity (ORAC) assays. The aqueous extract of the leaves was found to contain higher level of total polyphenols (11.63±0.023 mg GAE/g), flavanol (1.45±0.10 mg CE/g), and flavonol (2.22±0.37 mg QE/g) than the extract of the fruits with values of 1.75±0.02 mg GAE/g (total polyphenol), 0.15±0.01 mg CE/g (flavanol), and 1.00±0.13 mg QE/g (flavonol). Similarly, the aqueous extract of the leaves also exhibited higher DPPH (IC50 20.64 mg/mL), FRAP (86.10±3.46 μmol AAE/g), TEAC (94.83±3.57 μmol TE/g), and ORAC (196.55±0.073 μmol TE/g) than the extract of the fruits with DPPH (IC50 120.33 mg/mL), FRAP (12.23±0.40 μmol AAE/g), TEAC (12.48±0.21 μmol TE/g), and ORAC (58.88±0.073 μmol TE/g). The present study showed that Nauclea latifolia has strong antioxidant potentials with the leaves demonstrating higher in vitro antioxidant activities than the fruits. |
| format | Article |
| id | doaj-art-367c51f4464943a3a352d3255c8df8f1 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-367c51f4464943a3a352d3255c8df8f12025-02-03T01:33:08ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/437081437081In Vitro Study on the Antioxidant Potentials of the Leaves and Fruits of Nauclea latifoliaAdemola O. Ayeleso0Oluwafemi O. Oguntibeju1Nicole L. Brooks2Oxidative Stress Research Centre, Department of Biomedical Sciences, Faculty of Health & Wellness Sciences, Cape Peninsula University of Technology, Bellville 7535, South AfricaOxidative Stress Research Centre, Department of Biomedical Sciences, Faculty of Health & Wellness Sciences, Cape Peninsula University of Technology, Bellville 7535, South AfricaDepartment of Wellness Sciences, Faculty of Health & Wellness Sciences, Cape Peninsula University of Technology, Cape Town 8000, South AfricaThis study was carried out to investigate the in vitro antioxidant potentials of the leaves and fruits of Nauclea latifolia, a straggling shrub or small tree, native to tropical Africa and Asia. Hot water extracts of the leaves and fruits of Nauclea latifolia were assessed for their total polyphenolic, flavanol, and flavonol contents as well as 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability, ferric reducing antioxidant power (FRAP), Trolox equivalence antioxidant capacity (TEAC), and oxygen radical absorbance capacity (ORAC) assays. The aqueous extract of the leaves was found to contain higher level of total polyphenols (11.63±0.023 mg GAE/g), flavanol (1.45±0.10 mg CE/g), and flavonol (2.22±0.37 mg QE/g) than the extract of the fruits with values of 1.75±0.02 mg GAE/g (total polyphenol), 0.15±0.01 mg CE/g (flavanol), and 1.00±0.13 mg QE/g (flavonol). Similarly, the aqueous extract of the leaves also exhibited higher DPPH (IC50 20.64 mg/mL), FRAP (86.10±3.46 μmol AAE/g), TEAC (94.83±3.57 μmol TE/g), and ORAC (196.55±0.073 μmol TE/g) than the extract of the fruits with DPPH (IC50 120.33 mg/mL), FRAP (12.23±0.40 μmol AAE/g), TEAC (12.48±0.21 μmol TE/g), and ORAC (58.88±0.073 μmol TE/g). The present study showed that Nauclea latifolia has strong antioxidant potentials with the leaves demonstrating higher in vitro antioxidant activities than the fruits.http://dx.doi.org/10.1155/2014/437081 |
| spellingShingle | Ademola O. Ayeleso Oluwafemi O. Oguntibeju Nicole L. Brooks In Vitro Study on the Antioxidant Potentials of the Leaves and Fruits of Nauclea latifolia The Scientific World Journal |
| title | In Vitro Study on the Antioxidant Potentials of the Leaves and Fruits of Nauclea latifolia |
| title_full | In Vitro Study on the Antioxidant Potentials of the Leaves and Fruits of Nauclea latifolia |
| title_fullStr | In Vitro Study on the Antioxidant Potentials of the Leaves and Fruits of Nauclea latifolia |
| title_full_unstemmed | In Vitro Study on the Antioxidant Potentials of the Leaves and Fruits of Nauclea latifolia |
| title_short | In Vitro Study on the Antioxidant Potentials of the Leaves and Fruits of Nauclea latifolia |
| title_sort | in vitro study on the antioxidant potentials of the leaves and fruits of nauclea latifolia |
| url | http://dx.doi.org/10.1155/2014/437081 |
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