The Effect of the Application of Chemical Fertilizer and Arbuscular MyCorrhizal Fungi on Maize Yield and Soil Microbiota in Saline Agricultural Soil

The overuse of chemical fertilizers not only leads to resource wastage but also causes problems such as environmental pollution and soil degradation. In particular, crop growth in saline–sodic soils is severely restricted due to high salinity and alkalinity, further exacerbating challenges in agricu...

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Main Authors: Ye Yuan, Zhengjun Feng, Shengxin Yan, Junjie Zhang, Huiping Song, Yan Zou, Dapeng Jin
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Journal of Fungi
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Online Access:https://www.mdpi.com/2309-608X/11/4/319
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author Ye Yuan
Zhengjun Feng
Shengxin Yan
Junjie Zhang
Huiping Song
Yan Zou
Dapeng Jin
author_facet Ye Yuan
Zhengjun Feng
Shengxin Yan
Junjie Zhang
Huiping Song
Yan Zou
Dapeng Jin
author_sort Ye Yuan
collection DOAJ
description The overuse of chemical fertilizers not only leads to resource wastage but also causes problems such as environmental pollution and soil degradation. In particular, crop growth in saline–sodic soils is severely restricted due to high salinity and alkalinity, further exacerbating challenges in agricultural production. The aim of this study was to investigate different fertilization strategies that combine chemical fertilizer reduction with arbuscular mycorrhizal fungi (AMF) for improving saline–sodic soils and to assess the effects of these protocols on crop yield, soil properties, and microbial communities. Field experiments across two sites (BeiWuLao and XuJiaZhen) demonstrated that integrating AMF with CF reduction (AHCF treatment) significantly enhanced maize yield by 23.5% at BeiWuLao (from 11,475 to 14,175 kg/ha) and 81.2% at XuJiaZhen (from 7245 to 13,125 kg/ha) compared to conventional fertilization (CK) (<i>p</i> < 0.01). Soil nutrient analysis revealed substantial improvements: available potassium (AK) increased by 77.7% (61.35 vs. 39.33 mg/kg), available phosphorus (AP) by 33.9% (20.50 vs. 15.50 mg/kg), ammonium nitrogen (AN) by 57.3% (64.17 vs. 40.83 mg/kg), and soil organic matter (SOM) by 96.4% (46.98 vs. 23.91 mg/kg) under AHCF treatment (<i>p</i> < 0.05). Although pH and electrical conductivity (ECe) remained unaffected, AMF inoculation shifted microbial composition, elevating salinity-tolerant taxa such as Actinobacteria (+24.7%) and Anabaena. Beta diversity analysis (PCoA) confirmed distinct microbial community structures between treatments, with ECe and AN identified as primary drivers of bacterial (RDA variance: 74.08%) and fungal (RDA variance: 54.63%) communities, respectively. Overall, the combination of chemical fertilizer reduction and AMF effectively improved soil fertility, microbial community structure, and crop yield. These findings have important implications for improving saline soils and promoting environmental sustainability.
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spelling doaj-art-3668c72389b74babb07ea10de0f1514c2025-08-20T02:18:03ZengMDPI AGJournal of Fungi2309-608X2025-04-0111431910.3390/jof11040319The Effect of the Application of Chemical Fertilizer and Arbuscular MyCorrhizal Fungi on Maize Yield and Soil Microbiota in Saline Agricultural SoilYe Yuan0Zhengjun Feng1Shengxin Yan2Junjie Zhang3Huiping Song4Yan Zou5Dapeng Jin6Institute of Resources and Environmental Engineering, Shanxi University, Taiyuan 030006, ChinaInstitute of Resources and Environmental Engineering, Shanxi University, Taiyuan 030006, ChinaInstitute of Resources and Environmental Engineering, Shanxi University, Taiyuan 030006, ChinaInstitute of Resources and Environmental Engineering, Shanxi University, Taiyuan 030006, ChinaInstitute of Resources and Environmental Engineering, Shanxi University, Taiyuan 030006, ChinaShanxi Qinghuan Nengchuang Environmental Protection Technology Co., Ltd., Taiyuan 030006, ChinaShanxi Qinghuan Nengchuang Environmental Protection Technology Co., Ltd., Taiyuan 030006, ChinaThe overuse of chemical fertilizers not only leads to resource wastage but also causes problems such as environmental pollution and soil degradation. In particular, crop growth in saline–sodic soils is severely restricted due to high salinity and alkalinity, further exacerbating challenges in agricultural production. The aim of this study was to investigate different fertilization strategies that combine chemical fertilizer reduction with arbuscular mycorrhizal fungi (AMF) for improving saline–sodic soils and to assess the effects of these protocols on crop yield, soil properties, and microbial communities. Field experiments across two sites (BeiWuLao and XuJiaZhen) demonstrated that integrating AMF with CF reduction (AHCF treatment) significantly enhanced maize yield by 23.5% at BeiWuLao (from 11,475 to 14,175 kg/ha) and 81.2% at XuJiaZhen (from 7245 to 13,125 kg/ha) compared to conventional fertilization (CK) (<i>p</i> < 0.01). Soil nutrient analysis revealed substantial improvements: available potassium (AK) increased by 77.7% (61.35 vs. 39.33 mg/kg), available phosphorus (AP) by 33.9% (20.50 vs. 15.50 mg/kg), ammonium nitrogen (AN) by 57.3% (64.17 vs. 40.83 mg/kg), and soil organic matter (SOM) by 96.4% (46.98 vs. 23.91 mg/kg) under AHCF treatment (<i>p</i> < 0.05). Although pH and electrical conductivity (ECe) remained unaffected, AMF inoculation shifted microbial composition, elevating salinity-tolerant taxa such as Actinobacteria (+24.7%) and Anabaena. Beta diversity analysis (PCoA) confirmed distinct microbial community structures between treatments, with ECe and AN identified as primary drivers of bacterial (RDA variance: 74.08%) and fungal (RDA variance: 54.63%) communities, respectively. Overall, the combination of chemical fertilizer reduction and AMF effectively improved soil fertility, microbial community structure, and crop yield. These findings have important implications for improving saline soils and promoting environmental sustainability.https://www.mdpi.com/2309-608X/11/4/319chemical fertilizerarbuscular mycorrhizal fungi (AMF)saline–sodic soilsoil microbial community
spellingShingle Ye Yuan
Zhengjun Feng
Shengxin Yan
Junjie Zhang
Huiping Song
Yan Zou
Dapeng Jin
The Effect of the Application of Chemical Fertilizer and Arbuscular MyCorrhizal Fungi on Maize Yield and Soil Microbiota in Saline Agricultural Soil
Journal of Fungi
chemical fertilizer
arbuscular mycorrhizal fungi (AMF)
saline–sodic soil
soil microbial community
title The Effect of the Application of Chemical Fertilizer and Arbuscular MyCorrhizal Fungi on Maize Yield and Soil Microbiota in Saline Agricultural Soil
title_full The Effect of the Application of Chemical Fertilizer and Arbuscular MyCorrhizal Fungi on Maize Yield and Soil Microbiota in Saline Agricultural Soil
title_fullStr The Effect of the Application of Chemical Fertilizer and Arbuscular MyCorrhizal Fungi on Maize Yield and Soil Microbiota in Saline Agricultural Soil
title_full_unstemmed The Effect of the Application of Chemical Fertilizer and Arbuscular MyCorrhizal Fungi on Maize Yield and Soil Microbiota in Saline Agricultural Soil
title_short The Effect of the Application of Chemical Fertilizer and Arbuscular MyCorrhizal Fungi on Maize Yield and Soil Microbiota in Saline Agricultural Soil
title_sort effect of the application of chemical fertilizer and arbuscular mycorrhizal fungi on maize yield and soil microbiota in saline agricultural soil
topic chemical fertilizer
arbuscular mycorrhizal fungi (AMF)
saline–sodic soil
soil microbial community
url https://www.mdpi.com/2309-608X/11/4/319
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