Resistin Is Increased in Periodontal Cells and Tissues: In Vitro and In Vivo Studies
Resistin, a proinflammatory adipokine, is elevated in many inflammatory diseases. However, little is known about its performance in periodontitis. The present study is aimed at evaluating resistin expression and synthesis in periodontal cells and tissues under inflammatory/microbial stress in additi...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/9817095 |
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| author | Andressa V. B. Nogueira Marjan Nokhbehsaim Sema Tekin Rafael S. de Molon Luis C. Spolidorio Svenja Memmert Anna Damanaki Andreas Jäger Sigrun Eick James Deschner Joni A. Cirelli |
| author_facet | Andressa V. B. Nogueira Marjan Nokhbehsaim Sema Tekin Rafael S. de Molon Luis C. Spolidorio Svenja Memmert Anna Damanaki Andreas Jäger Sigrun Eick James Deschner Joni A. Cirelli |
| author_sort | Andressa V. B. Nogueira |
| collection | DOAJ |
| description | Resistin, a proinflammatory adipokine, is elevated in many inflammatory diseases. However, little is known about its performance in periodontitis. The present study is aimed at evaluating resistin expression and synthesis in periodontal cells and tissues under inflammatory/microbial stress in addition to its effects on the periodontium. In vivo, 24 male rats were randomly divided into two groups: control and ligature-induced periodontal disease. After 6 and 12 days, animals were sacrificed to analyze gene expression of adipokines, bone loss, inflammation, and resistin synthesis. In vitro, human periodontal ligament (PDL) fibroblasts were used to evaluate the expression of resistin after inflammatory stimuli. In addition, PDL fibroblasts were exposed to resistin to evaluate its role on soft and hard tissue metabolism markers. The periodontitis group demonstrated significant bone loss, an increase in the number of inflammatory cells and vascular structures, an increase in resistin expression and synthesis, and a decrease in the expression of adiponectin, leptin, and its functional receptor. PDL fibroblasts showed a significant increase in resistin expression and synthesis in response to the inflammatory stimulus by IL-1β. Resistin induced an increase in cytokine expression and a decrease in the regulation of some hard tissue and matrix formation genes in PDL fibroblasts. These data indicate that resistin is produced by periodontal cells and tissues, and this effect is enhanced by inflammatory stimuli. Moreover, resistin seems to interfere with soft and hard tissue metabolism during periodontitis by reducing markers related to matrix formation and bone tissue. |
| format | Article |
| id | doaj-art-365cf934f39445949bbf9b0fc03dd16f |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-365cf934f39445949bbf9b0fc03dd16f2025-08-20T02:08:15ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/98170959817095Resistin Is Increased in Periodontal Cells and Tissues: In Vitro and In Vivo StudiesAndressa V. B. Nogueira0Marjan Nokhbehsaim1Sema Tekin2Rafael S. de Molon3Luis C. Spolidorio4Svenja Memmert5Anna Damanaki6Andreas Jäger7Sigrun Eick8James Deschner9Joni A. Cirelli10Department of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University, Mainz 55131, GermanySection of Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn 53111, GermanyPrivate Practice, Istanbul, Turkey, Formerly Clinical Research Unit 208, University of Bonn, Bonn 53111, GermanyDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University-UNESP, Araraquara, São Paulo 14801-903, BrazilDepartment of Physiology and Pathology, School of Dentistry at Araraquara, São Paulo State University-UNESP, Araraquara, São Paulo 14801-903, BrazilSection of Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn 53111, GermanyDepartment of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University, Mainz 55131, GermanyDepartment of Orthodontics, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn 53111, GermanyDepartment of Periodontology, Laboratory of Oral Microbiology, University of Bern, Bern 3012, SwitzerlandDepartment of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University, Mainz 55131, GermanyDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University-UNESP, Araraquara, São Paulo 14801-903, BrazilResistin, a proinflammatory adipokine, is elevated in many inflammatory diseases. However, little is known about its performance in periodontitis. The present study is aimed at evaluating resistin expression and synthesis in periodontal cells and tissues under inflammatory/microbial stress in addition to its effects on the periodontium. In vivo, 24 male rats were randomly divided into two groups: control and ligature-induced periodontal disease. After 6 and 12 days, animals were sacrificed to analyze gene expression of adipokines, bone loss, inflammation, and resistin synthesis. In vitro, human periodontal ligament (PDL) fibroblasts were used to evaluate the expression of resistin after inflammatory stimuli. In addition, PDL fibroblasts were exposed to resistin to evaluate its role on soft and hard tissue metabolism markers. The periodontitis group demonstrated significant bone loss, an increase in the number of inflammatory cells and vascular structures, an increase in resistin expression and synthesis, and a decrease in the expression of adiponectin, leptin, and its functional receptor. PDL fibroblasts showed a significant increase in resistin expression and synthesis in response to the inflammatory stimulus by IL-1β. Resistin induced an increase in cytokine expression and a decrease in the regulation of some hard tissue and matrix formation genes in PDL fibroblasts. These data indicate that resistin is produced by periodontal cells and tissues, and this effect is enhanced by inflammatory stimuli. Moreover, resistin seems to interfere with soft and hard tissue metabolism during periodontitis by reducing markers related to matrix formation and bone tissue.http://dx.doi.org/10.1155/2020/9817095 |
| spellingShingle | Andressa V. B. Nogueira Marjan Nokhbehsaim Sema Tekin Rafael S. de Molon Luis C. Spolidorio Svenja Memmert Anna Damanaki Andreas Jäger Sigrun Eick James Deschner Joni A. Cirelli Resistin Is Increased in Periodontal Cells and Tissues: In Vitro and In Vivo Studies Mediators of Inflammation |
| title | Resistin Is Increased in Periodontal Cells and Tissues: In Vitro and In Vivo Studies |
| title_full | Resistin Is Increased in Periodontal Cells and Tissues: In Vitro and In Vivo Studies |
| title_fullStr | Resistin Is Increased in Periodontal Cells and Tissues: In Vitro and In Vivo Studies |
| title_full_unstemmed | Resistin Is Increased in Periodontal Cells and Tissues: In Vitro and In Vivo Studies |
| title_short | Resistin Is Increased in Periodontal Cells and Tissues: In Vitro and In Vivo Studies |
| title_sort | resistin is increased in periodontal cells and tissues in vitro and in vivo studies |
| url | http://dx.doi.org/10.1155/2020/9817095 |
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