Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey

The aim of this study was to provide molecular detection and characterization of the goat isolate of Taenia hydatigena from Ankara province of Turkey. For this purpose, PCR amplification of small subunit ribosomal RNA (rrnS) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-...

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Main Authors: Armagan Erdem Utuk, Fatma Cigdem Piskin
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/2012/962732
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author Armagan Erdem Utuk
Fatma Cigdem Piskin
author_facet Armagan Erdem Utuk
Fatma Cigdem Piskin
author_sort Armagan Erdem Utuk
collection DOAJ
description The aim of this study was to provide molecular detection and characterization of the goat isolate of Taenia hydatigena from Ankara province of Turkey. For this purpose, PCR amplification of small subunit ribosomal RNA (rrnS) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed in a one-month-old dead goat. According to rrnS-PCR results, parasites were identified as Taenia spp., and partial sequence of mt-CO1 gene was corresponding to T. hydatigena. At the end of the study, we concluded that molecular tools can be used to define species of parasites in cases where the key morphologic features cannot be detected. Nucleotide sequence data of Turkish goat isolate of T. hydatigena was submitted to GenBank for other researchers interested in this subject. By this study, molecular detection and characterization of T. hydatigena was done for the first time in Turkey.
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spelling doaj-art-3657cf5485994dc19b6275bdc343677f2025-02-03T06:06:04ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/962732962732Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in TurkeyArmagan Erdem Utuk0Fatma Cigdem Piskin1Veterinary Control Central Research Institute, Parasitology and Bee Diseases Laboratory, 06020 Ankara, TurkeyVeterinary Control Central Research Institute, Parasitology and Bee Diseases Laboratory, 06020 Ankara, TurkeyThe aim of this study was to provide molecular detection and characterization of the goat isolate of Taenia hydatigena from Ankara province of Turkey. For this purpose, PCR amplification of small subunit ribosomal RNA (rrnS) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed in a one-month-old dead goat. According to rrnS-PCR results, parasites were identified as Taenia spp., and partial sequence of mt-CO1 gene was corresponding to T. hydatigena. At the end of the study, we concluded that molecular tools can be used to define species of parasites in cases where the key morphologic features cannot be detected. Nucleotide sequence data of Turkish goat isolate of T. hydatigena was submitted to GenBank for other researchers interested in this subject. By this study, molecular detection and characterization of T. hydatigena was done for the first time in Turkey.http://dx.doi.org/10.1100/2012/962732
spellingShingle Armagan Erdem Utuk
Fatma Cigdem Piskin
Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey
The Scientific World Journal
title Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey
title_full Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey
title_fullStr Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey
title_full_unstemmed Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey
title_short Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey
title_sort molecular detection and characterization of goat isolate of taenia hydatigena in turkey
url http://dx.doi.org/10.1100/2012/962732
work_keys_str_mv AT armaganerdemutuk moleculardetectionandcharacterizationofgoatisolateoftaeniahydatigenainturkey
AT fatmacigdempiskin moleculardetectionandcharacterizationofgoatisolateoftaeniahydatigenainturkey