Olfactory sensory neurons transiently express multiple olfactory receptors during development
Abstract In mammals, each olfactory sensory neuron randomly expresses one, and only one, olfactory receptor (OR)—a phenomenon called the “one‐neuron‐one‐receptor” rule. Although extensively studied, this rule was never proven for all ~1,000 OR genes in one cell at once, and little is known about its...
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Springer Nature
2015-12-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.15252/msb.20156639 |
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| _version_ | 1849235765491400704 |
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| author | Longzhi Tan Qian Li X Sunney Xie |
| author_facet | Longzhi Tan Qian Li X Sunney Xie |
| author_sort | Longzhi Tan |
| collection | DOAJ |
| description | Abstract In mammals, each olfactory sensory neuron randomly expresses one, and only one, olfactory receptor (OR)—a phenomenon called the “one‐neuron‐one‐receptor” rule. Although extensively studied, this rule was never proven for all ~1,000 OR genes in one cell at once, and little is known about its dynamics. Here, we directly tested this rule by single‐cell transcriptomic sequencing of 178 cells from the main olfactory epithelium of adult and newborn mice. To our surprise, a subset of cells expressed multiple ORs. Most of these cells were developmentally immature. Our results illustrated how the “one‐neuron‐one‐receptor” rule may have been established: At first, a single neuron temporarily expressed multiple ORs—seemingly violating the rule—and then all but one OR were eliminated. This work provided experimental evidence that epigenetic regulation in the olfactory system selects a single OR by suppressing a few transiently expressed ORs in a single cell during development. |
| format | Article |
| id | doaj-art-3642d64accdb4cabb7934e9833205eda |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2015-12-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-3642d64accdb4cabb7934e9833205eda2025-08-20T04:02:41ZengSpringer NatureMolecular Systems Biology1744-42922015-12-0111121910.15252/msb.20156639Olfactory sensory neurons transiently express multiple olfactory receptors during developmentLongzhi Tan0Qian Li1X Sunney Xie2Department of Chemistry and Chemical Biology, Harvard UniversityDepartment of Cell Biology, Harvard Medical SchoolDepartment of Chemistry and Chemical Biology, Harvard UniversityAbstract In mammals, each olfactory sensory neuron randomly expresses one, and only one, olfactory receptor (OR)—a phenomenon called the “one‐neuron‐one‐receptor” rule. Although extensively studied, this rule was never proven for all ~1,000 OR genes in one cell at once, and little is known about its dynamics. Here, we directly tested this rule by single‐cell transcriptomic sequencing of 178 cells from the main olfactory epithelium of adult and newborn mice. To our surprise, a subset of cells expressed multiple ORs. Most of these cells were developmentally immature. Our results illustrated how the “one‐neuron‐one‐receptor” rule may have been established: At first, a single neuron temporarily expressed multiple ORs—seemingly violating the rule—and then all but one OR were eliminated. This work provided experimental evidence that epigenetic regulation in the olfactory system selects a single OR by suppressing a few transiently expressed ORs in a single cell during development.https://doi.org/10.15252/msb.20156639immature and mature olfactory sensory neuronsodorant receptorolfactory systemsingle‐cell RNA‐Seqtrace amine‐associated receptor (TAAR) |
| spellingShingle | Longzhi Tan Qian Li X Sunney Xie Olfactory sensory neurons transiently express multiple olfactory receptors during development Molecular Systems Biology immature and mature olfactory sensory neurons odorant receptor olfactory system single‐cell RNA‐Seq trace amine‐associated receptor (TAAR) |
| title | Olfactory sensory neurons transiently express multiple olfactory receptors during development |
| title_full | Olfactory sensory neurons transiently express multiple olfactory receptors during development |
| title_fullStr | Olfactory sensory neurons transiently express multiple olfactory receptors during development |
| title_full_unstemmed | Olfactory sensory neurons transiently express multiple olfactory receptors during development |
| title_short | Olfactory sensory neurons transiently express multiple olfactory receptors during development |
| title_sort | olfactory sensory neurons transiently express multiple olfactory receptors during development |
| topic | immature and mature olfactory sensory neurons odorant receptor olfactory system single‐cell RNA‐Seq trace amine‐associated receptor (TAAR) |
| url | https://doi.org/10.15252/msb.20156639 |
| work_keys_str_mv | AT longzhitan olfactorysensoryneuronstransientlyexpressmultipleolfactoryreceptorsduringdevelopment AT qianli olfactorysensoryneuronstransientlyexpressmultipleolfactoryreceptorsduringdevelopment AT xsunneyxie olfactorysensoryneuronstransientlyexpressmultipleolfactoryreceptorsduringdevelopment |