Effect of green coffee on miR-133a, miR-155 and inflammatory biomarkers in obese individuals
Abstract Objectives Metabolic syndrome is a cluster of conditions that increases the risk of atherosclerotic cardiovascular diseases. The current study was a randomized, double blind, placebo-controlled study that aimed to determine the impact of green coffee (GC) in obese patients with metabolic sy...
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BMC
2024-10-01
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| Series: | Diabetology & Metabolic Syndrome |
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| Online Access: | https://doi.org/10.1186/s13098-024-01478-7 |
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| author | Naglaa F. Khedr Enas S. Zahran Abla M. Ebeid Samuel T. Melek Rehab H. Werida |
| author_facet | Naglaa F. Khedr Enas S. Zahran Abla M. Ebeid Samuel T. Melek Rehab H. Werida |
| author_sort | Naglaa F. Khedr |
| collection | DOAJ |
| description | Abstract Objectives Metabolic syndrome is a cluster of conditions that increases the risk of atherosclerotic cardiovascular diseases. The current study was a randomized, double blind, placebo-controlled study that aimed to determine the impact of green coffee (GC) in obese patients with metabolic syndrome through analysis of miRNA-155, miRNA-133a and the inflammatory biomarkers such as resistin, TNF-α, total sialic acid, homocysteine, high sensitivity C-reactive protein (hs-CRP), and the anti-inflammatory cytokine, adiponectin. Methods One hundred-sixty obese patients were randomly supplemented either with GC capsules (800 mg) or placebo daily for six months. Both groups were advised to take a balanced diet. Blood samples were collected at baseline and after six months of supplementation. Results GC supplementation for 6 months reduced BMI (p = 0.002), waist circumference (p = 0.038), blood glucose (p = 0.002), HbA1c% (p = 0.000), Insulin (p = 0.000), systolic blood pressure (p = 0.005), diastolic BP (p = 0.001) compared with placebo. GC significantly decreased total cholesterol (TC, p = 0.000), LDL-C (p = 0.001), triglycerides (TG, p = 0.002) and increased HDL-C (p = 0.008) compared with placebo group. In addition, GC significantly (p ≤ 0.005) reduced total sialic acid, homocysteine, resistin, TNF-α, hs-CRP and the oxidative stress marker malondialdehyde (MDA), but increased serum adiponectin (p = 0.000) compared to placebo group. There was a significant reduction in the gene expression of miR-133a (p = 0.000) in GC group as compared with baseline levels and with the control placebo group (p = 0.001) after 6 months. Conclusion GC administration modulated metabolic syndrome by decreasing BMI, high BP, blood glucose, dyslipidemia, miRNA-133a and inflammatory biomarkers that constitute risk factors for cardiovascular diseases. ClinicalTrials.gov registration No. is NCT05688917. Graphical Abstract |
| format | Article |
| id | doaj-art-36407ae952bc4258b57f1ceb26ca8c4f |
| institution | OA Journals |
| issn | 1758-5996 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Diabetology & Metabolic Syndrome |
| spelling | doaj-art-36407ae952bc4258b57f1ceb26ca8c4f2025-08-20T02:18:31ZengBMCDiabetology & Metabolic Syndrome1758-59962024-10-0116111610.1186/s13098-024-01478-7Effect of green coffee on miR-133a, miR-155 and inflammatory biomarkers in obese individualsNaglaa F. Khedr0Enas S. Zahran1Abla M. Ebeid2Samuel T. Melek3Rehab H. Werida4Biochemistry Department, Faculty of Pharmacy, Medical Complex, Tanta UniversityInternal Medicine Department, Faculty of Medicine, Menoufia UniversityClinical Pharmacy Department, Faculty of Pharmacy, AL-Delta UniversityDepartment of Parasitology and Blood Research at National Organization for Drug Control and Research (NODCAR)Clinical Pharmacy Department, Faculty of Pharmacy, Damanhour UniversityAbstract Objectives Metabolic syndrome is a cluster of conditions that increases the risk of atherosclerotic cardiovascular diseases. The current study was a randomized, double blind, placebo-controlled study that aimed to determine the impact of green coffee (GC) in obese patients with metabolic syndrome through analysis of miRNA-155, miRNA-133a and the inflammatory biomarkers such as resistin, TNF-α, total sialic acid, homocysteine, high sensitivity C-reactive protein (hs-CRP), and the anti-inflammatory cytokine, adiponectin. Methods One hundred-sixty obese patients were randomly supplemented either with GC capsules (800 mg) or placebo daily for six months. Both groups were advised to take a balanced diet. Blood samples were collected at baseline and after six months of supplementation. Results GC supplementation for 6 months reduced BMI (p = 0.002), waist circumference (p = 0.038), blood glucose (p = 0.002), HbA1c% (p = 0.000), Insulin (p = 0.000), systolic blood pressure (p = 0.005), diastolic BP (p = 0.001) compared with placebo. GC significantly decreased total cholesterol (TC, p = 0.000), LDL-C (p = 0.001), triglycerides (TG, p = 0.002) and increased HDL-C (p = 0.008) compared with placebo group. In addition, GC significantly (p ≤ 0.005) reduced total sialic acid, homocysteine, resistin, TNF-α, hs-CRP and the oxidative stress marker malondialdehyde (MDA), but increased serum adiponectin (p = 0.000) compared to placebo group. There was a significant reduction in the gene expression of miR-133a (p = 0.000) in GC group as compared with baseline levels and with the control placebo group (p = 0.001) after 6 months. Conclusion GC administration modulated metabolic syndrome by decreasing BMI, high BP, blood glucose, dyslipidemia, miRNA-133a and inflammatory biomarkers that constitute risk factors for cardiovascular diseases. ClinicalTrials.gov registration No. is NCT05688917. Graphical Abstracthttps://doi.org/10.1186/s13098-024-01478-7AdiponectinGreen coffeeMetabolic syndromemiR-133amiR-155Resistin |
| spellingShingle | Naglaa F. Khedr Enas S. Zahran Abla M. Ebeid Samuel T. Melek Rehab H. Werida Effect of green coffee on miR-133a, miR-155 and inflammatory biomarkers in obese individuals Diabetology & Metabolic Syndrome Adiponectin Green coffee Metabolic syndrome miR-133a miR-155 Resistin |
| title | Effect of green coffee on miR-133a, miR-155 and inflammatory biomarkers in obese individuals |
| title_full | Effect of green coffee on miR-133a, miR-155 and inflammatory biomarkers in obese individuals |
| title_fullStr | Effect of green coffee on miR-133a, miR-155 and inflammatory biomarkers in obese individuals |
| title_full_unstemmed | Effect of green coffee on miR-133a, miR-155 and inflammatory biomarkers in obese individuals |
| title_short | Effect of green coffee on miR-133a, miR-155 and inflammatory biomarkers in obese individuals |
| title_sort | effect of green coffee on mir 133a mir 155 and inflammatory biomarkers in obese individuals |
| topic | Adiponectin Green coffee Metabolic syndrome miR-133a miR-155 Resistin |
| url | https://doi.org/10.1186/s13098-024-01478-7 |
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