Applicability of Oral Fluid and Dried Blood Spot for Hepatitis B Virus Diagnosis
Hepatitis B virus (HBV) is one of the major causes of chronic liver disease worldwide; however most of individuals are not aware about the infection. Oral fluid and dried blood spot (DBS) samples may be an alternative to serum to HBV diagnosis to increase the access to diagnosis in remote areas or h...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Canadian Journal of Gastroenterology and Hepatology |
| Online Access: | http://dx.doi.org/10.1155/2019/5672795 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551868937535488 |
|---|---|
| author | Livia Melo Villar Cristianne Sousa Bezerra Helena Medina Cruz Moyra Machado Portilho Geane Lopes Flores |
| author_facet | Livia Melo Villar Cristianne Sousa Bezerra Helena Medina Cruz Moyra Machado Portilho Geane Lopes Flores |
| author_sort | Livia Melo Villar |
| collection | DOAJ |
| description | Hepatitis B virus (HBV) is one of the major causes of chronic liver disease worldwide; however most of individuals are not aware about the infection. Oral fluid and dried blood spot (DBS) samples may be an alternative to serum to HBV diagnosis to increase the access to diagnosis in remote areas or high-risk groups. The main objective of this review is to give an insight about the usefulness of oral fluid and DBS for detecting HBV markers. Several groups have evaluated the detection of HBsAg, anti-HBc, and anti-HBs markers in oral fluid and DBS samples demonstrating 13 to 100% of sensitivity and specificity according different groups, sample collectors, and diagnosis assays. In the same way, HBV DNA detection using oral fluid and DBS samples demonstrate different values of sensitivity according type of collection, studied group, extraction, and detection methods. Thus, serological and molecular diagnostic tests demonstrated good performance for detecting HBV using oral fluid and DBS according some characteristics and could be useful to increase the access to the diagnosis of HBV. |
| format | Article |
| id | doaj-art-36390fb9f8db48e9be9c1140f000b321 |
| institution | Kabale University |
| issn | 2291-2789 2291-2797 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Gastroenterology and Hepatology |
| spelling | doaj-art-36390fb9f8db48e9be9c1140f000b3212025-02-03T06:00:10ZengWileyCanadian Journal of Gastroenterology and Hepatology2291-27892291-27972019-01-01201910.1155/2019/56727955672795Applicability of Oral Fluid and Dried Blood Spot for Hepatitis B Virus DiagnosisLivia Melo Villar0Cristianne Sousa Bezerra1Helena Medina Cruz2Moyra Machado Portilho3Geane Lopes Flores4Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, BrazilFederal Institute of Education, Science and Technology of Ceará, Campus Fortaleza, Ceará, BrazilLaboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, BrazilInstituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, BrazilLaboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, BrazilHepatitis B virus (HBV) is one of the major causes of chronic liver disease worldwide; however most of individuals are not aware about the infection. Oral fluid and dried blood spot (DBS) samples may be an alternative to serum to HBV diagnosis to increase the access to diagnosis in remote areas or high-risk groups. The main objective of this review is to give an insight about the usefulness of oral fluid and DBS for detecting HBV markers. Several groups have evaluated the detection of HBsAg, anti-HBc, and anti-HBs markers in oral fluid and DBS samples demonstrating 13 to 100% of sensitivity and specificity according different groups, sample collectors, and diagnosis assays. In the same way, HBV DNA detection using oral fluid and DBS samples demonstrate different values of sensitivity according type of collection, studied group, extraction, and detection methods. Thus, serological and molecular diagnostic tests demonstrated good performance for detecting HBV using oral fluid and DBS according some characteristics and could be useful to increase the access to the diagnosis of HBV.http://dx.doi.org/10.1155/2019/5672795 |
| spellingShingle | Livia Melo Villar Cristianne Sousa Bezerra Helena Medina Cruz Moyra Machado Portilho Geane Lopes Flores Applicability of Oral Fluid and Dried Blood Spot for Hepatitis B Virus Diagnosis Canadian Journal of Gastroenterology and Hepatology |
| title | Applicability of Oral Fluid and Dried Blood Spot for Hepatitis B Virus Diagnosis |
| title_full | Applicability of Oral Fluid and Dried Blood Spot for Hepatitis B Virus Diagnosis |
| title_fullStr | Applicability of Oral Fluid and Dried Blood Spot for Hepatitis B Virus Diagnosis |
| title_full_unstemmed | Applicability of Oral Fluid and Dried Blood Spot for Hepatitis B Virus Diagnosis |
| title_short | Applicability of Oral Fluid and Dried Blood Spot for Hepatitis B Virus Diagnosis |
| title_sort | applicability of oral fluid and dried blood spot for hepatitis b virus diagnosis |
| url | http://dx.doi.org/10.1155/2019/5672795 |
| work_keys_str_mv | AT liviamelovillar applicabilityoforalfluidanddriedbloodspotforhepatitisbvirusdiagnosis AT cristiannesousabezerra applicabilityoforalfluidanddriedbloodspotforhepatitisbvirusdiagnosis AT helenamedinacruz applicabilityoforalfluidanddriedbloodspotforhepatitisbvirusdiagnosis AT moyramachadoportilho applicabilityoforalfluidanddriedbloodspotforhepatitisbvirusdiagnosis AT geanelopesflores applicabilityoforalfluidanddriedbloodspotforhepatitisbvirusdiagnosis |