Optimal Nitrogen Accumulation and Remobilization Can Synergistically Improve Maize Yield and Nitrogen-Use Efficiency Under Low-Nitrogen Conditions

Increasing the nitrogen (N) use efficiency (NUE) of modern high-yield maize hybrids is essential for food security and reducing environmental risks. However, the relationship between dry matter (DM), N accumulation, and reallocation among different high-yield maize hybrids and NUE, particularly unde...

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Main Authors: Xiang Li, Lin Piao, Wenhao Duan, Yan Bai, Nanheng Zhu, Qingquan Tang, Fangming He, Hong Ren, Yan Gu
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Agronomy
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Online Access:https://www.mdpi.com/2073-4395/15/5/1159
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author Xiang Li
Lin Piao
Wenhao Duan
Yan Bai
Nanheng Zhu
Qingquan Tang
Fangming He
Hong Ren
Yan Gu
author_facet Xiang Li
Lin Piao
Wenhao Duan
Yan Bai
Nanheng Zhu
Qingquan Tang
Fangming He
Hong Ren
Yan Gu
author_sort Xiang Li
collection DOAJ
description Increasing the nitrogen (N) use efficiency (NUE) of modern high-yield maize hybrids is essential for food security and reducing environmental risks. However, the relationship between dry matter (DM), N accumulation, and reallocation among different high-yield maize hybrids and NUE, particularly under various N fertilization levels, is not well understood. The field experiment was conducted in Jilin Province, Northeast China. In this study, two maize hybrids, Zhengdan958 (ZD958) and Tie 391 (T391), were grown under four N fertilizer levels: 0 (NN), 120 (LN), 240 (MN), and 360 (HN) kg ha<sup>−1</sup>. We examined the effects of N input on grain yield, NUE, DM, and N accumulation, partitioning, and reallocation of these two high-yielding maize hybrids during the 2023–2024 growing season. The results showed that N input significantly increased grain yield but reduced NUE. There was no significant difference in yield and NUE between the two maize hybrids at the HN level. However, under LN conditions, the grain yield and NUE of ZD958 were higher by 16.2% and 15.6%, respectively, compared to T391. Meanwhile, ZD958 exhibited greater per-silking and post-silking DM (5.0% and 7.9%) and N accumulation (11.6% and 32.7%), as well as a higher amount of reallocated DM (45.6%) and N (17.5%) compared to T391. Moreover, 15.5–38.1% of grain N for ZD958 and 17.2–46.7% for T391 still needed to be reallocated from vegetative organs, with a larger fraction coming from the stem rather than the leaves. The middle leaves and lower stems of the canopy tended to reallocate more N to the grain, and lower-layer stem N reallocation was significantly related to grain yield. In conclusion, higher accumulation of DM and N, along with greater N reallocation—especially from the lower-layer stem—could be regarded as important traits in maize breeding to improve the NUE of high-yield maize hybrids under insufficient N supply.
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series Agronomy
spelling doaj-art-3636d26a34ae41bbbc2daa24ba59fc9e2025-08-20T01:57:04ZengMDPI AGAgronomy2073-43952025-05-01155115910.3390/agronomy15051159Optimal Nitrogen Accumulation and Remobilization Can Synergistically Improve Maize Yield and Nitrogen-Use Efficiency Under Low-Nitrogen ConditionsXiang Li0Lin Piao1Wenhao Duan2Yan Bai3Nanheng Zhu4Qingquan Tang5Fangming He6Hong Ren7Yan Gu8Faculty of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaKey Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region, Northeast Agricultural University, Ministry of Education, Harbin 150030, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaFaculty of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaIncreasing the nitrogen (N) use efficiency (NUE) of modern high-yield maize hybrids is essential for food security and reducing environmental risks. However, the relationship between dry matter (DM), N accumulation, and reallocation among different high-yield maize hybrids and NUE, particularly under various N fertilization levels, is not well understood. The field experiment was conducted in Jilin Province, Northeast China. In this study, two maize hybrids, Zhengdan958 (ZD958) and Tie 391 (T391), were grown under four N fertilizer levels: 0 (NN), 120 (LN), 240 (MN), and 360 (HN) kg ha<sup>−1</sup>. We examined the effects of N input on grain yield, NUE, DM, and N accumulation, partitioning, and reallocation of these two high-yielding maize hybrids during the 2023–2024 growing season. The results showed that N input significantly increased grain yield but reduced NUE. There was no significant difference in yield and NUE between the two maize hybrids at the HN level. However, under LN conditions, the grain yield and NUE of ZD958 were higher by 16.2% and 15.6%, respectively, compared to T391. Meanwhile, ZD958 exhibited greater per-silking and post-silking DM (5.0% and 7.9%) and N accumulation (11.6% and 32.7%), as well as a higher amount of reallocated DM (45.6%) and N (17.5%) compared to T391. Moreover, 15.5–38.1% of grain N for ZD958 and 17.2–46.7% for T391 still needed to be reallocated from vegetative organs, with a larger fraction coming from the stem rather than the leaves. The middle leaves and lower stems of the canopy tended to reallocate more N to the grain, and lower-layer stem N reallocation was significantly related to grain yield. In conclusion, higher accumulation of DM and N, along with greater N reallocation—especially from the lower-layer stem—could be regarded as important traits in maize breeding to improve the NUE of high-yield maize hybrids under insufficient N supply.https://www.mdpi.com/2073-4395/15/5/1159N-use efficiencymaize hybridsN levelN accumulationreallocation
spellingShingle Xiang Li
Lin Piao
Wenhao Duan
Yan Bai
Nanheng Zhu
Qingquan Tang
Fangming He
Hong Ren
Yan Gu
Optimal Nitrogen Accumulation and Remobilization Can Synergistically Improve Maize Yield and Nitrogen-Use Efficiency Under Low-Nitrogen Conditions
Agronomy
N-use efficiency
maize hybrids
N level
N accumulation
reallocation
title Optimal Nitrogen Accumulation and Remobilization Can Synergistically Improve Maize Yield and Nitrogen-Use Efficiency Under Low-Nitrogen Conditions
title_full Optimal Nitrogen Accumulation and Remobilization Can Synergistically Improve Maize Yield and Nitrogen-Use Efficiency Under Low-Nitrogen Conditions
title_fullStr Optimal Nitrogen Accumulation and Remobilization Can Synergistically Improve Maize Yield and Nitrogen-Use Efficiency Under Low-Nitrogen Conditions
title_full_unstemmed Optimal Nitrogen Accumulation and Remobilization Can Synergistically Improve Maize Yield and Nitrogen-Use Efficiency Under Low-Nitrogen Conditions
title_short Optimal Nitrogen Accumulation and Remobilization Can Synergistically Improve Maize Yield and Nitrogen-Use Efficiency Under Low-Nitrogen Conditions
title_sort optimal nitrogen accumulation and remobilization can synergistically improve maize yield and nitrogen use efficiency under low nitrogen conditions
topic N-use efficiency
maize hybrids
N level
N accumulation
reallocation
url https://www.mdpi.com/2073-4395/15/5/1159
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