Gut Fungal Community Modulates Fat Deposition in Ningxiang Pigs: Species-Specific Regulation via the Glucose–SCFAs Metabolic Axis

Despite limited understanding of gut fungal roles in fat deposition among indigenous pig breeds, a comparative study between high-fat-accumulating Ningxiang (NX) pigs and lean-type Duroc × Landrace × Yorkshire (DLY) pigs reveals a fungal-driven regulatory mechanism. NX pigs exhibited significantly h...

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Main Authors: Pengfei Huang, Hanmin Wang, Juan Wang, Zhenrong Qiu, Chunfeng Wang, Han Liu, Qiye Wang, Yali Li, Huansheng Yang
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/13/1887
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Summary:Despite limited understanding of gut fungal roles in fat deposition among indigenous pig breeds, a comparative study between high-fat-accumulating Ningxiang (NX) pigs and lean-type Duroc × Landrace × Yorkshire (DLY) pigs reveals a fungal-driven regulatory mechanism. NX pigs exhibited significantly higher fat percentage, elevated serum glucose, and markedly reduced total colonic short-chain fatty acids (SCFAs) compared to DLY pigs (all <i>p</i> < 0.001), with butyrate showing the most pronounced decrease. Beta-diversity confirmed distinct fungal communities (<i>p</i> = 0.002), where NX pigs were enriched with <i>Aspergillus</i> and <i>Penicillium</i>, while DLY pigs harbored dominant <i>Rhodotorula</i>. Strong correlations were observed: NX-enriched fungi positively correlated with glucose and negatively with SCFAs, whereas <i>Rhodotorula</i> strongly associated with SCFAs (<i>p</i> < 0.001). FUNGuild analysis linked <i>Aspergillus</i>/<i>Penicillium</i> to enhanced polysaccharide degradation and glucose bioavailability. The findings propose a gut fungal-mediated “Glucose–SCFAs axis”: NX-enriched fungi elevate glucose (promoting lipogenesis) and suppress SCFAs (reducing butyrate-mediated adipocyte inhibition), whereas <i>Rhodotorula</i> in DLY pigs enhances SCFAs-induced lipolysis. Crucially, we demonstrate that fungal modulation primarily drives fat deposition differences between breeds, offering novel probiotics/antifungal strategies for precision swine breeding.
ISSN:2076-2615