The effects of different egg yolk concentrations used with soy bean lecithin-based extender on semen quality to freeze bull semen
<b>Aim:</b> The centrifuged egg yolk (CEY) was added at concentrations of 0.5 or 10% to a defined soy lecithin-based extender (Bioxcell<sup>®</sup>) used to freeze bull semen and their synergistic effects on post-thaw sperm motility, morphological abnormalities and membrane i...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
|
| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=558 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832543833968082944 |
|---|---|
| author | Serpil Sariozkan Purhan Barbaros Tuncer Mustafa Numan Bucak Serhat Buyukleblebici Huseyin Kinet Ali Bilgen |
| author_facet | Serpil Sariozkan Purhan Barbaros Tuncer Mustafa Numan Bucak Serhat Buyukleblebici Huseyin Kinet Ali Bilgen |
| author_sort | Serpil Sariozkan |
| collection | DOAJ |
| description | <b>Aim:</b> The centrifuged egg yolk (CEY) was added at concentrations
of 0.5 or 10% to a defined soy lecithin-based
extender (Bioxcell<sup>®</sup>) used to freeze bull semen and their
synergistic effects on post-thaw sperm motility, morphological
abnormalities and membrane integrity assessed.<p>
<b>Materials and Methods:</b> Ejaculates obtained from each
Simmental bull (n:12) divided in three equal aliquots
and diluted in CEY 5% (B5), 10% (B10) supplemented,
and without any CEY (B0) in soy bean lecithin - based
extender, respectively. Thereafter, they were frozen and
thawed following a standart protocol. Sperm cryosurvival
was evaluated in vitro by microscopic assessments
of post-thaw sperm motility (by means of the CASA),
acrosomal and other abnormalities (head, mid-pieces,
and tail) and plasma membrane integrity (evaluated by
HOST).<p>
<b>Results:</b> In Simmental bulls, semen extended with B5
had significantly higher CASA motility and CASA progressive
motility than those extended with the rest of
extenders after freezing and thawing (p<0.001). There
was no significant difference in VAP, VCL, and ALH
among the three groups (p>0.05). For VSL (p<0.01) and
LIN (p< 0.001), the highest values were obtained from
B10 group. The highest percentages of acrosomal and
other abnormalities were found in semen diluted in B10
(p<0.001). In the group frozen B5, the percentage of
membrane integrity was significantly higher than that of
the other groups (p<0.001).<p>
<b>Conclusion:</b> The use of CEY 5% in combination with soy
bean lecithin significantly improved bull semen freezability. |
| format | Article |
| id | doaj-art-363499aa0f1346edb1fee8bb2e992102 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-363499aa0f1346edb1fee8bb2e9921022025-02-03T11:25:42ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532614549558The effects of different egg yolk concentrations used with soy bean lecithin-based extender on semen quality to freeze bull semenSerpil Sariozkan0Purhan Barbaros Tuncer1Mustafa Numan Bucak2Serhat Buyukleblebici3Huseyin Kinet4Ali Bilgen5Erciyes University, Safiye Cikrikcioglu Vocational College, Kayseri, TurkeyMinistry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Ankara, TurkeyMinistry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Ankara, TurkeyMinistry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Ankara, TurkeyMinistry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Ankara, TurkeyMinistry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Ankara, Turkey<b>Aim:</b> The centrifuged egg yolk (CEY) was added at concentrations of 0.5 or 10% to a defined soy lecithin-based extender (Bioxcell<sup>®</sup>) used to freeze bull semen and their synergistic effects on post-thaw sperm motility, morphological abnormalities and membrane integrity assessed.<p> <b>Materials and Methods:</b> Ejaculates obtained from each Simmental bull (n:12) divided in three equal aliquots and diluted in CEY 5% (B5), 10% (B10) supplemented, and without any CEY (B0) in soy bean lecithin - based extender, respectively. Thereafter, they were frozen and thawed following a standart protocol. Sperm cryosurvival was evaluated in vitro by microscopic assessments of post-thaw sperm motility (by means of the CASA), acrosomal and other abnormalities (head, mid-pieces, and tail) and plasma membrane integrity (evaluated by HOST).<p> <b>Results:</b> In Simmental bulls, semen extended with B5 had significantly higher CASA motility and CASA progressive motility than those extended with the rest of extenders after freezing and thawing (p<0.001). There was no significant difference in VAP, VCL, and ALH among the three groups (p>0.05). For VSL (p<0.01) and LIN (p< 0.001), the highest values were obtained from B10 group. The highest percentages of acrosomal and other abnormalities were found in semen diluted in B10 (p<0.001). In the group frozen B5, the percentage of membrane integrity was significantly higher than that of the other groups (p<0.001).<p> <b>Conclusion:</b> The use of CEY 5% in combination with soy bean lecithin significantly improved bull semen freezability.http://eurasianjvetsci.org/pdf.php3?id=558soy bean lecithinegg yolkcryopreservationsemen parametersbull |
| spellingShingle | Serpil Sariozkan Purhan Barbaros Tuncer Mustafa Numan Bucak Serhat Buyukleblebici Huseyin Kinet Ali Bilgen The effects of different egg yolk concentrations used with soy bean lecithin-based extender on semen quality to freeze bull semen Eurasian Journal of Veterinary Sciences soy bean lecithin egg yolk cryopreservation semen parameters bull |
| title | The effects of different egg yolk concentrations used with soy bean lecithin-based extender on semen quality to freeze bull semen |
| title_full | The effects of different egg yolk concentrations used with soy bean lecithin-based extender on semen quality to freeze bull semen |
| title_fullStr | The effects of different egg yolk concentrations used with soy bean lecithin-based extender on semen quality to freeze bull semen |
| title_full_unstemmed | The effects of different egg yolk concentrations used with soy bean lecithin-based extender on semen quality to freeze bull semen |
| title_short | The effects of different egg yolk concentrations used with soy bean lecithin-based extender on semen quality to freeze bull semen |
| title_sort | effects of different egg yolk concentrations used with soy bean lecithin based extender on semen quality to freeze bull semen |
| topic | soy bean lecithin egg yolk cryopreservation semen parameters bull |
| url | http://eurasianjvetsci.org/pdf.php3?id=558 |
| work_keys_str_mv | AT serpilsariozkan theeffectsofdifferenteggyolkconcentrationsusedwithsoybeanlecithinbasedextenderonsemenqualitytofreezebullsemen AT purhanbarbarostuncer theeffectsofdifferenteggyolkconcentrationsusedwithsoybeanlecithinbasedextenderonsemenqualitytofreezebullsemen AT mustafanumanbucak theeffectsofdifferenteggyolkconcentrationsusedwithsoybeanlecithinbasedextenderonsemenqualitytofreezebullsemen AT serhatbuyukleblebici theeffectsofdifferenteggyolkconcentrationsusedwithsoybeanlecithinbasedextenderonsemenqualitytofreezebullsemen AT huseyinkinet theeffectsofdifferenteggyolkconcentrationsusedwithsoybeanlecithinbasedextenderonsemenqualitytofreezebullsemen AT alibilgen theeffectsofdifferenteggyolkconcentrationsusedwithsoybeanlecithinbasedextenderonsemenqualitytofreezebullsemen AT serpilsariozkan effectsofdifferenteggyolkconcentrationsusedwithsoybeanlecithinbasedextenderonsemenqualitytofreezebullsemen AT purhanbarbarostuncer effectsofdifferenteggyolkconcentrationsusedwithsoybeanlecithinbasedextenderonsemenqualitytofreezebullsemen AT mustafanumanbucak effectsofdifferenteggyolkconcentrationsusedwithsoybeanlecithinbasedextenderonsemenqualitytofreezebullsemen AT serhatbuyukleblebici effectsofdifferenteggyolkconcentrationsusedwithsoybeanlecithinbasedextenderonsemenqualitytofreezebullsemen AT huseyinkinet effectsofdifferenteggyolkconcentrationsusedwithsoybeanlecithinbasedextenderonsemenqualitytofreezebullsemen AT alibilgen effectsofdifferenteggyolkconcentrationsusedwithsoybeanlecithinbasedextenderonsemenqualitytofreezebullsemen |