Diel changes in the expression of a marker gene and candidate genes for intracellular amorphous CaCO3 biomineralization in Microcystis
Phylogenetically diverse cyanobacteria biomineralize intracellular amorphous calcium carbonate (iACC) inclusions. This includes several genotypes of the Microcystis genus, a potentially toxic, bloom-forming cyanobacterium found worldwide in freshwater ecosystems. While we ignore the biological funct...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Peer Community In
2025-02-01
|
| Series: | Peer Community Journal |
| Subjects: | |
| Online Access: | https://peercommunityjournal.org/articles/10.24072/pcjournal.516/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825206347241619456 |
|---|---|
| author | Bruley, Apolline Gaëtan, Juliette Gugger, Muriel Pancrace, Claire Millet, Maxime Gaschignard, Geoffroy Dezi, Manuela Humbert, Jean-François Leloup, Julie Skouri-Panet, Fériel Callebaut, Isabelle Benzerara, Karim Duprat, Elodie |
| author_facet | Bruley, Apolline Gaëtan, Juliette Gugger, Muriel Pancrace, Claire Millet, Maxime Gaschignard, Geoffroy Dezi, Manuela Humbert, Jean-François Leloup, Julie Skouri-Panet, Fériel Callebaut, Isabelle Benzerara, Karim Duprat, Elodie |
| author_sort | Bruley, Apolline |
| collection | DOAJ |
| description | Phylogenetically diverse cyanobacteria biomineralize intracellular amorphous calcium carbonate (iACC) inclusions. This includes several genotypes of the Microcystis genus, a potentially toxic, bloom-forming cyanobacterium found worldwide in freshwater ecosystems. While we ignore the biological function of iACC and the molecular mechanisms driving their formation, this process may impact local geochemical cycles and/or be used for bioremediation strategies. Recently, a marker gene of this biomineralization pathway, named ccyA, was discovered. However, the function of the calcyanin protein encoded by ccyA remains unknown. Here, based on an RNA-Seq approach, we assess the expression of the ccyA gene in Microcystis aeruginosa PCC 7806 during a 24 h day/night cycle. The ccyA gene shows a clear day/night expression pattern with maximum transcript abundances during the second half of the night. This is consistent with the assumption that iACC biomineralization is related with photosynthesis and may therefore follow a day/night cycle as well. Moreover, several genes directly co-localized upstream and downstream of ccyA, on the same DNA strand show a similar expression pattern, including a cax gene encoding a calcium/proton exchanger and a gene encoding a protein with a domain also present in the N-terminal region of calcyanins in many iACC-forming cyanobacteria. This suggests that they all could be part of an operon, and may play a concerted role in iACC formation. Last, several other genes involved in carbon concentrating mechanisms and calcium transport show an expression pattern similar to that of ccyA. Overall, this study provides a list of candidate genes that may be involved in the biomineralization of iACC by cyanobacteria and whose role could be, in the future, analyzed by biochemistry and genetics approaches. |
| format | Article |
| id | doaj-art-3633619c2bb7411d9b1ea104ee71facc |
| institution | Kabale University |
| issn | 2804-3871 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Peer Community In |
| record_format | Article |
| series | Peer Community Journal |
| spelling | doaj-art-3633619c2bb7411d9b1ea104ee71facc2025-02-07T10:34:51ZengPeer Community InPeer Community Journal2804-38712025-02-01510.24072/pcjournal.51610.24072/pcjournal.516Diel changes in the expression of a marker gene and candidate genes for intracellular amorphous CaCO3 biomineralization in Microcystis Bruley, Apolline0Gaëtan, Juliette1Gugger, Muriel2https://orcid.org/0000-0001-6728-1976Pancrace, Claire3Millet, Maxime4Gaschignard, Geoffroy5Dezi, Manuela6https://orcid.org/0000-0001-8727-9910Humbert, Jean-François7Leloup, Julie8https://orcid.org/0000-0002-2777-284XSkouri-Panet, Fériel9Callebaut, Isabelle10Benzerara, Karim11https://orcid.org/0000-0002-0553-0137Duprat, Elodie12https://orcid.org/0000-0003-4150-6967Sorbonne Université, Muséum National d’Histoire Naturelle, UMR CNRS 7590, Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie, IMPMC – Paris, FranceSorbonne Université, Muséum National d’Histoire Naturelle, UMR CNRS 7590, Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie, IMPMC – Paris, FranceInstitut Pasteur, Université Paris Cité, Collection of Cyanobacteria – Paris, FranceInstitut Pasteur, Université Paris Cité, Collection of Cyanobacteria – Paris, France; Sorbonne Université, CNRS, INRAE, IRD, Université Paris Est Créteil, Université Paris Cité, UMR 7618. Institut d’Ecologie et des Sciences de l’Environnement de Paris (iEES-Paris) – Paris, FranceSorbonne Université, Muséum National d’Histoire Naturelle, UMR CNRS 7590, Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie, IMPMC – Paris, FranceSorbonne Université, Muséum National d’Histoire Naturelle, UMR CNRS 7590, Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie, IMPMC – Paris, FranceSorbonne Université, Muséum National d’Histoire Naturelle, UMR CNRS 7590, Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie, IMPMC – Paris, FranceSorbonne Université, CNRS, INRAE, IRD, Université Paris Est Créteil, Université Paris Cité, UMR 7618. Institut d’Ecologie et des Sciences de l’Environnement de Paris (iEES-Paris) – Paris, FranceSorbonne Université, CNRS, INRAE, IRD, Université Paris Est Créteil, Université Paris Cité, UMR 7618. Institut d’Ecologie et des Sciences de l’Environnement de Paris (iEES-Paris) – Paris, FranceSorbonne Université, Muséum National d’Histoire Naturelle, UMR CNRS 7590, Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie, IMPMC – Paris, FranceSorbonne Université, Muséum National d’Histoire Naturelle, UMR CNRS 7590, Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie, IMPMC – Paris, FranceSorbonne Université, Muséum National d’Histoire Naturelle, UMR CNRS 7590, Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie, IMPMC – Paris, FranceSorbonne Université, Muséum National d’Histoire Naturelle, UMR CNRS 7590, Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie, IMPMC – Paris, FrancePhylogenetically diverse cyanobacteria biomineralize intracellular amorphous calcium carbonate (iACC) inclusions. This includes several genotypes of the Microcystis genus, a potentially toxic, bloom-forming cyanobacterium found worldwide in freshwater ecosystems. While we ignore the biological function of iACC and the molecular mechanisms driving their formation, this process may impact local geochemical cycles and/or be used for bioremediation strategies. Recently, a marker gene of this biomineralization pathway, named ccyA, was discovered. However, the function of the calcyanin protein encoded by ccyA remains unknown. Here, based on an RNA-Seq approach, we assess the expression of the ccyA gene in Microcystis aeruginosa PCC 7806 during a 24 h day/night cycle. The ccyA gene shows a clear day/night expression pattern with maximum transcript abundances during the second half of the night. This is consistent with the assumption that iACC biomineralization is related with photosynthesis and may therefore follow a day/night cycle as well. Moreover, several genes directly co-localized upstream and downstream of ccyA, on the same DNA strand show a similar expression pattern, including a cax gene encoding a calcium/proton exchanger and a gene encoding a protein with a domain also present in the N-terminal region of calcyanins in many iACC-forming cyanobacteria. This suggests that they all could be part of an operon, and may play a concerted role in iACC formation. Last, several other genes involved in carbon concentrating mechanisms and calcium transport show an expression pattern similar to that of ccyA. Overall, this study provides a list of candidate genes that may be involved in the biomineralization of iACC by cyanobacteria and whose role could be, in the future, analyzed by biochemistry and genetics approaches.https://peercommunityjournal.org/articles/10.24072/pcjournal.516/Biomineralization, amorphous calcium carbonate, cyanobacteria, RNA-seq, cax gene, CCM |
| spellingShingle | Bruley, Apolline Gaëtan, Juliette Gugger, Muriel Pancrace, Claire Millet, Maxime Gaschignard, Geoffroy Dezi, Manuela Humbert, Jean-François Leloup, Julie Skouri-Panet, Fériel Callebaut, Isabelle Benzerara, Karim Duprat, Elodie Diel changes in the expression of a marker gene and candidate genes for intracellular amorphous CaCO3 biomineralization in Microcystis Peer Community Journal Biomineralization, amorphous calcium carbonate, cyanobacteria, RNA-seq, cax gene, CCM |
| title | Diel changes in the expression of a marker gene and candidate genes for intracellular amorphous CaCO3 biomineralization in Microcystis
|
| title_full | Diel changes in the expression of a marker gene and candidate genes for intracellular amorphous CaCO3 biomineralization in Microcystis
|
| title_fullStr | Diel changes in the expression of a marker gene and candidate genes for intracellular amorphous CaCO3 biomineralization in Microcystis
|
| title_full_unstemmed | Diel changes in the expression of a marker gene and candidate genes for intracellular amorphous CaCO3 biomineralization in Microcystis
|
| title_short | Diel changes in the expression of a marker gene and candidate genes for intracellular amorphous CaCO3 biomineralization in Microcystis
|
| title_sort | diel changes in the expression of a marker gene and candidate genes for intracellular amorphous caco3 biomineralization in microcystis |
| topic | Biomineralization, amorphous calcium carbonate, cyanobacteria, RNA-seq, cax gene, CCM |
| url | https://peercommunityjournal.org/articles/10.24072/pcjournal.516/ |
| work_keys_str_mv | AT bruleyapolline dielchangesintheexpressionofamarkergeneandcandidategenesforintracellularamorphouscaco3biomineralizationinmicrocystis AT gaetanjuliette dielchangesintheexpressionofamarkergeneandcandidategenesforintracellularamorphouscaco3biomineralizationinmicrocystis AT guggermuriel dielchangesintheexpressionofamarkergeneandcandidategenesforintracellularamorphouscaco3biomineralizationinmicrocystis AT pancraceclaire dielchangesintheexpressionofamarkergeneandcandidategenesforintracellularamorphouscaco3biomineralizationinmicrocystis AT milletmaxime dielchangesintheexpressionofamarkergeneandcandidategenesforintracellularamorphouscaco3biomineralizationinmicrocystis AT gaschignardgeoffroy dielchangesintheexpressionofamarkergeneandcandidategenesforintracellularamorphouscaco3biomineralizationinmicrocystis AT dezimanuela dielchangesintheexpressionofamarkergeneandcandidategenesforintracellularamorphouscaco3biomineralizationinmicrocystis AT humbertjeanfrancois dielchangesintheexpressionofamarkergeneandcandidategenesforintracellularamorphouscaco3biomineralizationinmicrocystis AT leloupjulie dielchangesintheexpressionofamarkergeneandcandidategenesforintracellularamorphouscaco3biomineralizationinmicrocystis AT skouripanetferiel dielchangesintheexpressionofamarkergeneandcandidategenesforintracellularamorphouscaco3biomineralizationinmicrocystis AT callebautisabelle dielchangesintheexpressionofamarkergeneandcandidategenesforintracellularamorphouscaco3biomineralizationinmicrocystis AT benzerarakarim dielchangesintheexpressionofamarkergeneandcandidategenesforintracellularamorphouscaco3biomineralizationinmicrocystis AT dupratelodie dielchangesintheexpressionofamarkergeneandcandidategenesforintracellularamorphouscaco3biomineralizationinmicrocystis |