Leveraging RIBOTAC technology: Fluorescent RNase L probes for live-cell imaging and function analysis
RNA-targeting small molecules, particularly RIBOnuclease TArgeting Chimeras (RIBOTACs), represent a powerful and promising therapeutic approach by selectively degrading RNAs through ribonuclease (RNase) recruitment. Despite their potential, the development of effective RNase recruitment tools is sti...
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Elsevier
2025-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024173264 |
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| author | Elias Khaskia Raphael I. Benhamou |
| author_facet | Elias Khaskia Raphael I. Benhamou |
| author_sort | Elias Khaskia |
| collection | DOAJ |
| description | RNA-targeting small molecules, particularly RIBOnuclease TArgeting Chimeras (RIBOTACs), represent a powerful and promising therapeutic approach by selectively degrading RNAs through ribonuclease (RNase) recruitment. Despite their potential, the development of effective RNase recruitment tools is still in its early stages and remains a critical area of research. Ribonuclease L (RNase L) is a key ribonuclease targeted by RIBOTACs, yet the tools available for studying RNase L are limited. In this study, we introduce novel fluorescent ribonuclease binders that enhance the visualization and investigation of RNase L activity. Our findings provide new insights into RNase L dynamics and RNA degradation pathways, paving the way for more effective RNA-targeted degradation strategies. Furthermore, we explore the versatility of these conjugates for real-time tracking of RNase L localization, intracellular trafficking, and mechanistic studies. These fluorescent probes also enable high-throughput fluorescence-based assays to identify small molecules that bind and recruit RNase L, advancing RNA-targeted therapeutic approaches. |
| format | Article |
| id | doaj-art-3624d5b1d73c4f7eb30a4c40411902fa |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-3624d5b1d73c4f7eb30a4c40411902fa2025-01-17T04:50:49ZengElsevierHeliyon2405-84402025-01-01111e41295Leveraging RIBOTAC technology: Fluorescent RNase L probes for live-cell imaging and function analysisElias Khaskia0Raphael I. Benhamou1The Institute for Drug Research of the School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, IsraelCorresponding author.; The Institute for Drug Research of the School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, IsraelRNA-targeting small molecules, particularly RIBOnuclease TArgeting Chimeras (RIBOTACs), represent a powerful and promising therapeutic approach by selectively degrading RNAs through ribonuclease (RNase) recruitment. Despite their potential, the development of effective RNase recruitment tools is still in its early stages and remains a critical area of research. Ribonuclease L (RNase L) is a key ribonuclease targeted by RIBOTACs, yet the tools available for studying RNase L are limited. In this study, we introduce novel fluorescent ribonuclease binders that enhance the visualization and investigation of RNase L activity. Our findings provide new insights into RNase L dynamics and RNA degradation pathways, paving the way for more effective RNA-targeted degradation strategies. Furthermore, we explore the versatility of these conjugates for real-time tracking of RNase L localization, intracellular trafficking, and mechanistic studies. These fluorescent probes also enable high-throughput fluorescence-based assays to identify small molecules that bind and recruit RNase L, advancing RNA-targeted therapeutic approaches.http://www.sciencedirect.com/science/article/pii/S2405844024173264RIBOTACRNase LFluorescent probesSubcellular localization |
| spellingShingle | Elias Khaskia Raphael I. Benhamou Leveraging RIBOTAC technology: Fluorescent RNase L probes for live-cell imaging and function analysis Heliyon RIBOTAC RNase L Fluorescent probes Subcellular localization |
| title | Leveraging RIBOTAC technology: Fluorescent RNase L probes for live-cell imaging and function analysis |
| title_full | Leveraging RIBOTAC technology: Fluorescent RNase L probes for live-cell imaging and function analysis |
| title_fullStr | Leveraging RIBOTAC technology: Fluorescent RNase L probes for live-cell imaging and function analysis |
| title_full_unstemmed | Leveraging RIBOTAC technology: Fluorescent RNase L probes for live-cell imaging and function analysis |
| title_short | Leveraging RIBOTAC technology: Fluorescent RNase L probes for live-cell imaging and function analysis |
| title_sort | leveraging ribotac technology fluorescent rnase l probes for live cell imaging and function analysis |
| topic | RIBOTAC RNase L Fluorescent probes Subcellular localization |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024173264 |
| work_keys_str_mv | AT eliaskhaskia leveragingribotactechnologyfluorescentrnaselprobesforlivecellimagingandfunctionanalysis AT raphaelibenhamou leveragingribotactechnologyfluorescentrnaselprobesforlivecellimagingandfunctionanalysis |