Isolation of a Novel Caprine <i>Eimeria christenseni</i> Strain (GC) in Canary Islands and Analysis of Parasitological, Clinical, and Pathological Findings on Experimentally Infected Goat Kids
<i>Eimeria christenseni</i> is considered among the most pathogenic <i>Eimeria</i> species in goats. The aim of this study was to isolate an <i>E. christenseni</i> strain and to assess its infectivity, pathogenicity, and ability to develop a protective immune resp...
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2025-01-01
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| author | Emilio Barba José Manuel Molina Francisco Rodríguez Otilia Ferrer María Carmen Muñoz Liliana M. R. Silva María Cristina Del Río José Adrián Molina Anja Taubert Carlos Hermosilla Antonio Ruiz |
| author_facet | Emilio Barba José Manuel Molina Francisco Rodríguez Otilia Ferrer María Carmen Muñoz Liliana M. R. Silva María Cristina Del Río José Adrián Molina Anja Taubert Carlos Hermosilla Antonio Ruiz |
| author_sort | Emilio Barba |
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| description | <i>Eimeria christenseni</i> is considered among the most pathogenic <i>Eimeria</i> species in goats. The aim of this study was to isolate an <i>E. christenseni</i> strain and to assess its infectivity, pathogenicity, and ability to develop a protective immune response. After previous collection of <i>E. christenseni</i>-positive faeces, purification of oocysts, and amplification in donor animals, an experimental infection was carried out. A total of 19 kids were divided into three groups: primary-infected and challenged, challenge control, and uninfected control. Infections were performed orally with 2 × 10<sup>5</sup> sporulated oocysts per animal. Oocyst shedding, clinical signs, and production parameters, in addition to haematological and histopathological features, were monitored. The results showed that the Gran Canaria (GC) <i>E. christenseni</i> strain had similar morphological and biological characteristics to those previously described, but no significant clinical signs were observed despite the high oocyst counts here recorded. The novel strain isolated would therefore be of low pathogenicity but still able to develop significant immunoprotective responses upon challenge infections. Its biological similarities to highly pathogenic species such as <i>Eimeria ninakohlyakimovae</i> and <i>Eimeria arloingi</i> might enable comparative studies aimed at developing alternative strategies for drug treatments, including <i>Eimeria</i> species (strain)-specific vaccination strategies for the efficient control of goat coccidiosis. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Animals |
| spelling | doaj-art-361e9f16568f471b885ec0f6af388d712025-01-24T13:17:43ZengMDPI AGAnimals2076-26152025-01-0115213910.3390/ani15020139Isolation of a Novel Caprine <i>Eimeria christenseni</i> Strain (GC) in Canary Islands and Analysis of Parasitological, Clinical, and Pathological Findings on Experimentally Infected Goat KidsEmilio Barba0José Manuel Molina1Francisco Rodríguez2Otilia Ferrer3María Carmen Muñoz4Liliana M. R. Silva5María Cristina Del Río6José Adrián Molina7Anja Taubert8Carlos Hermosilla9Antonio Ruiz10Department of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas of Gran Canaria, 35001 Las Palmas de Gran Canaria, SpainDepartment of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas of Gran Canaria, 35001 Las Palmas de Gran Canaria, SpainDepartment of Anatomy and Compared Anatomy Pathology, Faculty of Veterinary Medicine, University of Las Palmas of Gran Canaria, 35001 Las Palmas de Gran Canaria, SpainDepartment of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas of Gran Canaria, 35001 Las Palmas de Gran Canaria, SpainDepartment of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas of Gran Canaria, 35001 Las Palmas de Gran Canaria, SpainFaculty of Veterinary Medicine, Institute of Parasitology, Justus Liebig University Giessen, 35390 Giessen, GermanyDepartment of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas of Gran Canaria, 35001 Las Palmas de Gran Canaria, SpainDepartment of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas of Gran Canaria, 35001 Las Palmas de Gran Canaria, SpainFaculty of Veterinary Medicine, Institute of Parasitology, Justus Liebig University Giessen, 35390 Giessen, GermanyFaculty of Veterinary Medicine, Institute of Parasitology, Justus Liebig University Giessen, 35390 Giessen, GermanyDepartment of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas of Gran Canaria, 35001 Las Palmas de Gran Canaria, Spain<i>Eimeria christenseni</i> is considered among the most pathogenic <i>Eimeria</i> species in goats. The aim of this study was to isolate an <i>E. christenseni</i> strain and to assess its infectivity, pathogenicity, and ability to develop a protective immune response. After previous collection of <i>E. christenseni</i>-positive faeces, purification of oocysts, and amplification in donor animals, an experimental infection was carried out. A total of 19 kids were divided into three groups: primary-infected and challenged, challenge control, and uninfected control. Infections were performed orally with 2 × 10<sup>5</sup> sporulated oocysts per animal. Oocyst shedding, clinical signs, and production parameters, in addition to haematological and histopathological features, were monitored. The results showed that the Gran Canaria (GC) <i>E. christenseni</i> strain had similar morphological and biological characteristics to those previously described, but no significant clinical signs were observed despite the high oocyst counts here recorded. The novel strain isolated would therefore be of low pathogenicity but still able to develop significant immunoprotective responses upon challenge infections. Its biological similarities to highly pathogenic species such as <i>Eimeria ninakohlyakimovae</i> and <i>Eimeria arloingi</i> might enable comparative studies aimed at developing alternative strategies for drug treatments, including <i>Eimeria</i> species (strain)-specific vaccination strategies for the efficient control of goat coccidiosis.https://www.mdpi.com/2076-2615/15/2/139coccidiagoatsnew strain<i>Eimeria christenseni</i>experimental infection |
| spellingShingle | Emilio Barba José Manuel Molina Francisco Rodríguez Otilia Ferrer María Carmen Muñoz Liliana M. R. Silva María Cristina Del Río José Adrián Molina Anja Taubert Carlos Hermosilla Antonio Ruiz Isolation of a Novel Caprine <i>Eimeria christenseni</i> Strain (GC) in Canary Islands and Analysis of Parasitological, Clinical, and Pathological Findings on Experimentally Infected Goat Kids Animals coccidia goats new strain <i>Eimeria christenseni</i> experimental infection |
| title | Isolation of a Novel Caprine <i>Eimeria christenseni</i> Strain (GC) in Canary Islands and Analysis of Parasitological, Clinical, and Pathological Findings on Experimentally Infected Goat Kids |
| title_full | Isolation of a Novel Caprine <i>Eimeria christenseni</i> Strain (GC) in Canary Islands and Analysis of Parasitological, Clinical, and Pathological Findings on Experimentally Infected Goat Kids |
| title_fullStr | Isolation of a Novel Caprine <i>Eimeria christenseni</i> Strain (GC) in Canary Islands and Analysis of Parasitological, Clinical, and Pathological Findings on Experimentally Infected Goat Kids |
| title_full_unstemmed | Isolation of a Novel Caprine <i>Eimeria christenseni</i> Strain (GC) in Canary Islands and Analysis of Parasitological, Clinical, and Pathological Findings on Experimentally Infected Goat Kids |
| title_short | Isolation of a Novel Caprine <i>Eimeria christenseni</i> Strain (GC) in Canary Islands and Analysis of Parasitological, Clinical, and Pathological Findings on Experimentally Infected Goat Kids |
| title_sort | isolation of a novel caprine i eimeria christenseni i strain gc in canary islands and analysis of parasitological clinical and pathological findings on experimentally infected goat kids |
| topic | coccidia goats new strain <i>Eimeria christenseni</i> experimental infection |
| url | https://www.mdpi.com/2076-2615/15/2/139 |
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