Gene Expression Profile in Delay Graft Function: Inflammatory Markers Are Associated with Recipient and Donor Risk Factors
Background. Delayed graft function (DGF) remains an important problem after kidney transplantation and reduced long-term graft survival of the transplanted organ. The aim of the present study was to determine if the development of DGF was associated with a specific pattern of inflammatory gene expre...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/167361 |
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| author | Diego Guerrieri Luis Re Jorgelina Petroni Nella Ambrosi Roxana E. Pilotti H. Eduardo Chuluyan Domingo Casadei Claudio Incardona |
| author_facet | Diego Guerrieri Luis Re Jorgelina Petroni Nella Ambrosi Roxana E. Pilotti H. Eduardo Chuluyan Domingo Casadei Claudio Incardona |
| author_sort | Diego Guerrieri |
| collection | DOAJ |
| description | Background. Delayed graft function (DGF) remains an important problem after kidney transplantation and reduced long-term graft survival of the transplanted organ. The aim of the present study was to determine if the development of DGF was associated with a specific pattern of inflammatory gene expression in expanded criteria of deceased donor kidney transplantation. Also, we explored the presence of correlations between DGF risk factors and the profile that was found. Methods. Seven days after kidney transplant, a cDNA microarray was performed on biopsies of graft from patients with and without DGF. Data was confirmed by real-time PCR. Correlations were performed between inflammatory gene expression and clinical risk factors. Results. From a total of 84 genes analyzed, 58 genes were upregulated while only 1 gene was downregulated in patients with DGF compared with no DGF (P=0.01). The most relevant genes fold changes observed was IFNA1, IL-10, IL-1F7, IL-1R1, HMOX-1, and TGF-β. The results were confirmed for IFNA1, IL-1R1, HMOX-1 and TGF-β. A correlation was observed between TGF-β, donor age, and preablation creatinine, but not body mass index (BMI). Also, TGF-β showed an association with recipient age, while IFNA1 correlated with recipient BMI. Furthermore, TGF-β, IFNA1 and HMOX-1 correlated with several posttransplant kidney function markers, such as diuresis, ultrasound Doppler, and glycemia. Conclusions. Overall, the present study shows that DGF is associated with inflammatory markers, which are correlated with donor and recipient DGF risk factors. |
| format | Article |
| id | doaj-art-3619b214ca8743698eb9283f1dfa4ace |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-3619b214ca8743698eb9283f1dfa4ace2025-02-03T07:25:29ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/167361167361Gene Expression Profile in Delay Graft Function: Inflammatory Markers Are Associated with Recipient and Donor Risk FactorsDiego Guerrieri0Luis Re1Jorgelina Petroni2Nella Ambrosi3Roxana E. Pilotti4H. Eduardo Chuluyan5Domingo Casadei6Claudio Incardona7CEFYBO-School of Medicine, University of Buenos Aires, Paraguay 2155, 16th Floor, C1121ABG Buenos Aires, ArgentinaInstituto de Nefrología de Buenos Aires, Cabello 3889, C1425APQ Buenos Aires, ArgentinaInstituto de Nefrología de Buenos Aires, Cabello 3889, C1425APQ Buenos Aires, ArgentinaCEFYBO-School of Medicine, University of Buenos Aires, Paraguay 2155, 16th Floor, C1121ABG Buenos Aires, ArgentinaInstituto de Nefrología de Buenos Aires, Cabello 3889, C1425APQ Buenos Aires, ArgentinaCEFYBO-School of Medicine, University of Buenos Aires, Paraguay 2155, 16th Floor, C1121ABG Buenos Aires, ArgentinaInstituto de Nefrología de Buenos Aires, Cabello 3889, C1425APQ Buenos Aires, ArgentinaGADOR S.A., Darwin 429, C1414CUH Buenos Aires, ArgentinaBackground. Delayed graft function (DGF) remains an important problem after kidney transplantation and reduced long-term graft survival of the transplanted organ. The aim of the present study was to determine if the development of DGF was associated with a specific pattern of inflammatory gene expression in expanded criteria of deceased donor kidney transplantation. Also, we explored the presence of correlations between DGF risk factors and the profile that was found. Methods. Seven days after kidney transplant, a cDNA microarray was performed on biopsies of graft from patients with and without DGF. Data was confirmed by real-time PCR. Correlations were performed between inflammatory gene expression and clinical risk factors. Results. From a total of 84 genes analyzed, 58 genes were upregulated while only 1 gene was downregulated in patients with DGF compared with no DGF (P=0.01). The most relevant genes fold changes observed was IFNA1, IL-10, IL-1F7, IL-1R1, HMOX-1, and TGF-β. The results were confirmed for IFNA1, IL-1R1, HMOX-1 and TGF-β. A correlation was observed between TGF-β, donor age, and preablation creatinine, but not body mass index (BMI). Also, TGF-β showed an association with recipient age, while IFNA1 correlated with recipient BMI. Furthermore, TGF-β, IFNA1 and HMOX-1 correlated with several posttransplant kidney function markers, such as diuresis, ultrasound Doppler, and glycemia. Conclusions. Overall, the present study shows that DGF is associated with inflammatory markers, which are correlated with donor and recipient DGF risk factors.http://dx.doi.org/10.1155/2014/167361 |
| spellingShingle | Diego Guerrieri Luis Re Jorgelina Petroni Nella Ambrosi Roxana E. Pilotti H. Eduardo Chuluyan Domingo Casadei Claudio Incardona Gene Expression Profile in Delay Graft Function: Inflammatory Markers Are Associated with Recipient and Donor Risk Factors Mediators of Inflammation |
| title | Gene Expression Profile in Delay Graft Function: Inflammatory Markers Are Associated with Recipient and Donor Risk Factors |
| title_full | Gene Expression Profile in Delay Graft Function: Inflammatory Markers Are Associated with Recipient and Donor Risk Factors |
| title_fullStr | Gene Expression Profile in Delay Graft Function: Inflammatory Markers Are Associated with Recipient and Donor Risk Factors |
| title_full_unstemmed | Gene Expression Profile in Delay Graft Function: Inflammatory Markers Are Associated with Recipient and Donor Risk Factors |
| title_short | Gene Expression Profile in Delay Graft Function: Inflammatory Markers Are Associated with Recipient and Donor Risk Factors |
| title_sort | gene expression profile in delay graft function inflammatory markers are associated with recipient and donor risk factors |
| url | http://dx.doi.org/10.1155/2014/167361 |
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