Purified Clinoptilolite-Tuff as a Trap for Amines Associated with Chronic Wounds: Binding of Cadaverine, Putrescine, Histamines and Polyamines
Ulcerous lesions can arise in primary skin cancers and upon infiltration of the skin by malignant cells originating from other organs. These malignant fungating wounds are difficult to treat, and they cause pain, itching and malodor. Distressing malodor imposes a major burden on patients. The carrio...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Scientia Pharmaceutica |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-0532/93/1/7 |
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| Summary: | Ulcerous lesions can arise in primary skin cancers and upon infiltration of the skin by malignant cells originating from other organs. These malignant fungating wounds are difficult to treat, and they cause pain, itching and malodor. Distressing malodor imposes a major burden on patients. The carrion odor of decaying tissue is—at least in part—due to the bacterial breakdown products cadaverine and putrescine. Here, we examined the binding of cadaverine, histamine, putrescine, spermidine and spermine to the preparation of micronized purified clinoptilolite-tuff (PCT) by relying on three radiolabeled tracers ([<sup>3</sup>H]cadaverine, [<sup>3</sup>H]histamine and [<sup>3</sup>H]spermidine). Binding was rapid, stable and of high capacity. The binding affinities were in the low µM range. Displacement experiments indicated that the binding sites were non-equivalent. These three properties combined to support effective binding for any given ligand in the presence of the expected, submillimolar concentrations of competing ligands. This was further verified by measuring the binding of [<sup>3</sup>H]cadaverine in the presence of wound drainage fluids. [<sup>3</sup>H]Cadaverine was effectively adsorbed by a wound dressing, into which purified clinoptilolite-tuff had been incorporated: the observed binding capacity of this wound dressing was consistent with its content of purified clinoptilolite-tuff. Based on these findings, we propose that purified clinoptilolite-tuff be further investigated as a means to control malodor emanating from chronic wounds. |
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| ISSN: | 0036-8709 2218-0532 |