Ultrasonic/compound enzyme extraction, comparative characterisation and biological activity of Lonicera macranthoides polysaccharides
This study investigates two extraction methods—ultrasound-assisted extraction (UAE) and compound enzyme extraction (CE)—for the isolation of Lonicera macranthoides polysaccharides (LMP) and evaluates their physicochemical properties, bioactivity, and therapeutic potential. The optimization of extrac...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | Ultrasonics Sonochemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417725000380 |
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| author | Mengdie Ren Yiru Hou Danli Peng Hui Li Xuxiang Zhang Lei Qiao XiaoShuang Wang Yongmei Jiang FaMing Wu Gang Wang |
| author_facet | Mengdie Ren Yiru Hou Danli Peng Hui Li Xuxiang Zhang Lei Qiao XiaoShuang Wang Yongmei Jiang FaMing Wu Gang Wang |
| author_sort | Mengdie Ren |
| collection | DOAJ |
| description | This study investigates two extraction methods—ultrasound-assisted extraction (UAE) and compound enzyme extraction (CE)—for the isolation of Lonicera macranthoides polysaccharides (LMP) and evaluates their physicochemical properties, bioactivity, and therapeutic potential. The optimization of extraction conditions was performed using single-factor experiments and response surface methodology. The yield of LMP from UAE was optimized to 8.02 % ± 0.12 %, while the yield from CE was 10.03 % ± 0.32 %. Both polysaccharide extracts were characterized in terms of chemical composition, monosaccharide composition, molecular weight, thermal stability, and functional group analysis. Notably, UAE-LMP exhibited superior antioxidant, hypolipidemic, nitrite scavenging, and anti-tumor activities compared to CE-LMP. The ultrasound-assisted method enhanced the stability and bioactivity of LMP, with improved water- and oil-holding capacities and the integrity of the active groups. Our findings demonstrate that the extraction method significantly influences the structural and functional properties of LMP, suggesting that UAE may be a more effective approach for extracting bioactive polysaccharides with potential applications in medicine and nutrition. |
| format | Article |
| id | doaj-art-3610667013b34418a9a60951f9ace1c2 |
| institution | Kabale University |
| issn | 1350-4177 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-3610667013b34418a9a60951f9ace1c22025-02-11T04:33:38ZengElsevierUltrasonics Sonochemistry1350-41772025-03-01114107259Ultrasonic/compound enzyme extraction, comparative characterisation and biological activity of Lonicera macranthoides polysaccharidesMengdie Ren0Yiru Hou1Danli Peng2Hui Li3Xuxiang Zhang4Lei Qiao5XiaoShuang Wang6Yongmei Jiang7FaMing Wu8Gang Wang9School of Pharmacy, Zunyi Medical University, Zunyi 563000, Guizhou, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, ChinaSchool of Pharmacy, Zunyi Medical University, Zunyi 563000, Guizhou, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, ChinaSchool of Pharmacy, Zunyi Medical University, Zunyi 563000, Guizhou, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, ChinaSchool of Pharmacy, Zunyi Medical University, Zunyi 563000, Guizhou, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, ChinaSchool of Pharmacy, Zunyi Medical University, Zunyi 563000, Guizhou, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, ChinaSchool of Pharmacy, Zunyi Medical University, Zunyi 563000, Guizhou, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, ChinaSchool of Pharmacy, Zunyi Medical University, Zunyi 563000, Guizhou, ChinaSchool of Pharmacy, Zunyi Medical University, Zunyi 563000, Guizhou, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, ChinaSchool of Pharmacy, Zunyi Medical University, Zunyi 563000, Guizhou, China; Corresponding authors at: School of Pharmacy, Zunyi Medical University, Zunyi 563003, Guizhou, China.School of Pharmacy, Zunyi Medical University, Zunyi 563000, Guizhou, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, China; Corresponding authors at: School of Pharmacy, Zunyi Medical University, Zunyi 563003, Guizhou, China.This study investigates two extraction methods—ultrasound-assisted extraction (UAE) and compound enzyme extraction (CE)—for the isolation of Lonicera macranthoides polysaccharides (LMP) and evaluates their physicochemical properties, bioactivity, and therapeutic potential. The optimization of extraction conditions was performed using single-factor experiments and response surface methodology. The yield of LMP from UAE was optimized to 8.02 % ± 0.12 %, while the yield from CE was 10.03 % ± 0.32 %. Both polysaccharide extracts were characterized in terms of chemical composition, monosaccharide composition, molecular weight, thermal stability, and functional group analysis. Notably, UAE-LMP exhibited superior antioxidant, hypolipidemic, nitrite scavenging, and anti-tumor activities compared to CE-LMP. The ultrasound-assisted method enhanced the stability and bioactivity of LMP, with improved water- and oil-holding capacities and the integrity of the active groups. Our findings demonstrate that the extraction method significantly influences the structural and functional properties of LMP, suggesting that UAE may be a more effective approach for extracting bioactive polysaccharides with potential applications in medicine and nutrition.http://www.sciencedirect.com/science/article/pii/S1350417725000380PolysaccharideLonicera macranthoidesUltrasonicAntioxidantNitrite removalCholate binding Antitumor |
| spellingShingle | Mengdie Ren Yiru Hou Danli Peng Hui Li Xuxiang Zhang Lei Qiao XiaoShuang Wang Yongmei Jiang FaMing Wu Gang Wang Ultrasonic/compound enzyme extraction, comparative characterisation and biological activity of Lonicera macranthoides polysaccharides Ultrasonics Sonochemistry Polysaccharide Lonicera macranthoides Ultrasonic Antioxidant Nitrite removal Cholate binding Antitumor |
| title | Ultrasonic/compound enzyme extraction, comparative characterisation and biological activity of Lonicera macranthoides polysaccharides |
| title_full | Ultrasonic/compound enzyme extraction, comparative characterisation and biological activity of Lonicera macranthoides polysaccharides |
| title_fullStr | Ultrasonic/compound enzyme extraction, comparative characterisation and biological activity of Lonicera macranthoides polysaccharides |
| title_full_unstemmed | Ultrasonic/compound enzyme extraction, comparative characterisation and biological activity of Lonicera macranthoides polysaccharides |
| title_short | Ultrasonic/compound enzyme extraction, comparative characterisation and biological activity of Lonicera macranthoides polysaccharides |
| title_sort | ultrasonic compound enzyme extraction comparative characterisation and biological activity of lonicera macranthoides polysaccharides |
| topic | Polysaccharide Lonicera macranthoides Ultrasonic Antioxidant Nitrite removal Cholate binding Antitumor |
| url | http://www.sciencedirect.com/science/article/pii/S1350417725000380 |
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