Reduced Serum PD-L1 and Markers of Inflammation in Response to Alternate Day Fasting With a Low-Carbohydrate Intervention: A Secondary Analysis of a Single-Arm Trial
This secondary analysis aimed to examine the effect of a single-arm alternate day fasting intervention with a 30% low-carbohydrate diet on biomarkers of inflammation and immune activation in adults with obesity. A 12-week weight-loss period was followed by a 12-week weight maintenance period. Anthro...
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Elsevier
2025-03-01
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| Series: | Current Developments in Nutrition |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2475299125000253 |
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| author | Rand T Akasheh Giamila Fantuzzi Krista A Varady Ting-Yuan D Cheng Faiza Kalam |
| author_facet | Rand T Akasheh Giamila Fantuzzi Krista A Varady Ting-Yuan D Cheng Faiza Kalam |
| author_sort | Rand T Akasheh |
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| description | This secondary analysis aimed to examine the effect of a single-arm alternate day fasting intervention with a 30% low-carbohydrate diet on biomarkers of inflammation and immune activation in adults with obesity. A 12-week weight-loss period was followed by a 12-week weight maintenance period. Anthropometrics and blood samples were collected at baseline and weeks 12 and 24. Multiplex assay was used to measure serum biomarkers including programmed death ligand 1 (PD-L1), interleukin 8 (IL-8), IL-1 receptor antagonist (IL-1ra), chemokine ligand (CCL)2, CCL4, interferon gamma (IFnγ), IFNγ–induced protein 10 (IP-10), and cluster of differentiation 40 ligand (CD40-L). In 28 participants, body weight and fat mass decreased during the weight-loss period but stabilized during the weight maintenance period. Serum PD-L1 decreased from baseline to week 12 (P = 0.005) but not at week 24. Moreover, IL-1ra and CCL4 concentrations decreased from baseline to week 24 (P < 0.001 and P < 0.008, respectively). Changes were not significant for in CCL2, IL-8, CD40-L, IFNγ, or IP-10. In conclusion, alternate day fasting–low carbohydrate modulates circulating immune biomarkers, which may be relevant to diabetes, cancer, and autoimmunity.This trial was registered at clinicaltrials.gov as NCT03528317 (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934424/). |
| format | Article |
| id | doaj-art-360f3b4c6a444fcc953fc9f620dbd854 |
| institution | Kabale University |
| issn | 2475-2991 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Current Developments in Nutrition |
| spelling | doaj-art-360f3b4c6a444fcc953fc9f620dbd8542025-08-20T03:44:28ZengElsevierCurrent Developments in Nutrition2475-29912025-03-019310456610.1016/j.cdnut.2025.104566Reduced Serum PD-L1 and Markers of Inflammation in Response to Alternate Day Fasting With a Low-Carbohydrate Intervention: A Secondary Analysis of a Single-Arm TrialRand T Akasheh0Giamila Fantuzzi1Krista A Varady2Ting-Yuan D Cheng3Faiza Kalam4Division of Cancer Prevention and Control, Department of Internal Medicine, The Ohio State University Comprehensive Cancer Center – The James Cancer Hospital and Solove Research Institute, College of Medicine, The Ohio State University Wexner Medical Center, Columbus, OH, USADepartment of Kinesiology and Nutrition, College of Applied Health Sciences, University of Illinois Chicago, Chicago, IL, USADepartment of Kinesiology and Nutrition, College of Applied Health Sciences, University of Illinois Chicago, Chicago, IL, USADivision of Cancer Prevention and Control, Department of Internal Medicine, The Ohio State University Comprehensive Cancer Center – The James Cancer Hospital and Solove Research Institute, College of Medicine, The Ohio State University Wexner Medical Center, Columbus, OH, USA; Corresponding author.Division of Cancer Prevention and Control, Department of Internal Medicine, The Ohio State University Comprehensive Cancer Center – The James Cancer Hospital and Solove Research Institute, College of Medicine, The Ohio State University Wexner Medical Center, Columbus, OH, USAThis secondary analysis aimed to examine the effect of a single-arm alternate day fasting intervention with a 30% low-carbohydrate diet on biomarkers of inflammation and immune activation in adults with obesity. A 12-week weight-loss period was followed by a 12-week weight maintenance period. Anthropometrics and blood samples were collected at baseline and weeks 12 and 24. Multiplex assay was used to measure serum biomarkers including programmed death ligand 1 (PD-L1), interleukin 8 (IL-8), IL-1 receptor antagonist (IL-1ra), chemokine ligand (CCL)2, CCL4, interferon gamma (IFnγ), IFNγ–induced protein 10 (IP-10), and cluster of differentiation 40 ligand (CD40-L). In 28 participants, body weight and fat mass decreased during the weight-loss period but stabilized during the weight maintenance period. Serum PD-L1 decreased from baseline to week 12 (P = 0.005) but not at week 24. Moreover, IL-1ra and CCL4 concentrations decreased from baseline to week 24 (P < 0.001 and P < 0.008, respectively). Changes were not significant for in CCL2, IL-8, CD40-L, IFNγ, or IP-10. In conclusion, alternate day fasting–low carbohydrate modulates circulating immune biomarkers, which may be relevant to diabetes, cancer, and autoimmunity.This trial was registered at clinicaltrials.gov as NCT03528317 (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934424/).http://www.sciencedirect.com/science/article/pii/S2475299125000253Intermittent fastingalternate day fastinglow-carbohydrate dietobesityweight lossinflammation |
| spellingShingle | Rand T Akasheh Giamila Fantuzzi Krista A Varady Ting-Yuan D Cheng Faiza Kalam Reduced Serum PD-L1 and Markers of Inflammation in Response to Alternate Day Fasting With a Low-Carbohydrate Intervention: A Secondary Analysis of a Single-Arm Trial Current Developments in Nutrition Intermittent fasting alternate day fasting low-carbohydrate diet obesity weight loss inflammation |
| title | Reduced Serum PD-L1 and Markers of Inflammation in Response to Alternate Day Fasting With a Low-Carbohydrate Intervention: A Secondary Analysis of a Single-Arm Trial |
| title_full | Reduced Serum PD-L1 and Markers of Inflammation in Response to Alternate Day Fasting With a Low-Carbohydrate Intervention: A Secondary Analysis of a Single-Arm Trial |
| title_fullStr | Reduced Serum PD-L1 and Markers of Inflammation in Response to Alternate Day Fasting With a Low-Carbohydrate Intervention: A Secondary Analysis of a Single-Arm Trial |
| title_full_unstemmed | Reduced Serum PD-L1 and Markers of Inflammation in Response to Alternate Day Fasting With a Low-Carbohydrate Intervention: A Secondary Analysis of a Single-Arm Trial |
| title_short | Reduced Serum PD-L1 and Markers of Inflammation in Response to Alternate Day Fasting With a Low-Carbohydrate Intervention: A Secondary Analysis of a Single-Arm Trial |
| title_sort | reduced serum pd l1 and markers of inflammation in response to alternate day fasting with a low carbohydrate intervention a secondary analysis of a single arm trial |
| topic | Intermittent fasting alternate day fasting low-carbohydrate diet obesity weight loss inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S2475299125000253 |
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