Phylogenetic Patterns of Genus Megophrys (Anura: Megophryidae) from Java and Sumatra
The emergence of Java and Sumatra islands at the end of Miocene, has impact to the diversity of fauna that living in the islands. Amphibia, as one of a genetic information series, can be used as a good phylogenetic comparison. Genus Megophrys (Anura: Megophryidae), is a member of amphibians that liv...
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University of Brawijaya
2017-12-01
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| Series: | Journal of Experimental Life Science |
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| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/235 |
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| author | Nia Kurniawan Aminuddin Affandi Erintha Eka Wardani Indah Nur Chomsy Anggun Sausan Firdaus Agung Sih Kurnianto |
| author_facet | Nia Kurniawan Aminuddin Affandi Erintha Eka Wardani Indah Nur Chomsy Anggun Sausan Firdaus Agung Sih Kurnianto |
| author_sort | Nia Kurniawan |
| collection | DOAJ |
| description | The emergence of Java and Sumatra islands at the end of Miocene, has impact to the diversity of fauna that living in the islands. Amphibia, as one of a genetic information series, can be used as a good phylogenetic comparison. Genus Megophrys (Anura: Megophryidae), is a member of amphibians that live isolated at an altitude of 1000 ASL (Above Sea Level) or more. The purpose of this research is to analyze the phylogenetic patterns of genus Megophrys in Java and Sumatra based on the analysis of 16s rRNA gene. We also analyzed the zoogeography to understand the history of relationship and the establishment of these islands. We took samples on 12 localities: 10 areas of Sumatra and 2 areas of Java. We used Qiamp Blood and Tissue Kit to extract the DNA from tissues. Qualitative analysis of DNA was done by running electrophoresis on 1% agarose gel. Qualitative analysis of DNA was done by running electrophoresis on 1% agarose gel. Results show that Clade A has 2 subclasses: I and II. Subclade I consists of Sumatran specimens, whereas subclade II consists of Javanese specimens. Subclaude I and II have paraphyletic connections. Clade B classified as politomy clade consists of Megophrys sp. from West Pasaman, Megophrys sp. from West Sumatra and Megophrys sp. from Pasawaran, Lampung. The result shows that Megophrys sp. of subclade I (clade A) and clade B, belongs to the same group, both of them were originated from Sumatra. Megophrys sp. subclade I (clade A) originated from Tanggamus (Lampung) and West Lampung (Lampung) separated with 2 species of Megophrys sp. of clade B originated from Pasawaran (Lampung). |
| format | Article |
| id | doaj-art-35efcce33a5d4dbf9f4f385646c9b841 |
| institution | Kabale University |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2017-12-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Experimental Life Science |
| spelling | doaj-art-35efcce33a5d4dbf9f4f385646c9b8412025-08-20T03:47:45ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552017-12-01727683https://doi.org/10.21776/ub.jels.2017.007.02.03Phylogenetic Patterns of Genus Megophrys (Anura: Megophryidae) from Java and SumatraNia Kurniawan0Aminuddin Affandi1Erintha Eka Wardani2Indah Nur Chomsy3Anggun Sausan Firdaus4Agung Sih Kurnianto5Universitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaDepartement of Life Science, National Central University, TaiwanUniversitas BrawijayaThe emergence of Java and Sumatra islands at the end of Miocene, has impact to the diversity of fauna that living in the islands. Amphibia, as one of a genetic information series, can be used as a good phylogenetic comparison. Genus Megophrys (Anura: Megophryidae), is a member of amphibians that live isolated at an altitude of 1000 ASL (Above Sea Level) or more. The purpose of this research is to analyze the phylogenetic patterns of genus Megophrys in Java and Sumatra based on the analysis of 16s rRNA gene. We also analyzed the zoogeography to understand the history of relationship and the establishment of these islands. We took samples on 12 localities: 10 areas of Sumatra and 2 areas of Java. We used Qiamp Blood and Tissue Kit to extract the DNA from tissues. Qualitative analysis of DNA was done by running electrophoresis on 1% agarose gel. Qualitative analysis of DNA was done by running electrophoresis on 1% agarose gel. Results show that Clade A has 2 subclasses: I and II. Subclade I consists of Sumatran specimens, whereas subclade II consists of Javanese specimens. Subclaude I and II have paraphyletic connections. Clade B classified as politomy clade consists of Megophrys sp. from West Pasaman, Megophrys sp. from West Sumatra and Megophrys sp. from Pasawaran, Lampung. The result shows that Megophrys sp. of subclade I (clade A) and clade B, belongs to the same group, both of them were originated from Sumatra. Megophrys sp. subclade I (clade A) originated from Tanggamus (Lampung) and West Lampung (Lampung) separated with 2 species of Megophrys sp. of clade B originated from Pasawaran (Lampung).https://jels.ub.ac.id/index.php/jels/article/view/23516s rrnajavamegohpryssumatrazoogeography |
| spellingShingle | Nia Kurniawan Aminuddin Affandi Erintha Eka Wardani Indah Nur Chomsy Anggun Sausan Firdaus Agung Sih Kurnianto Phylogenetic Patterns of Genus Megophrys (Anura: Megophryidae) from Java and Sumatra Journal of Experimental Life Science 16s rrna java megohprys sumatra zoogeography |
| title | Phylogenetic Patterns of Genus Megophrys (Anura: Megophryidae) from Java and Sumatra |
| title_full | Phylogenetic Patterns of Genus Megophrys (Anura: Megophryidae) from Java and Sumatra |
| title_fullStr | Phylogenetic Patterns of Genus Megophrys (Anura: Megophryidae) from Java and Sumatra |
| title_full_unstemmed | Phylogenetic Patterns of Genus Megophrys (Anura: Megophryidae) from Java and Sumatra |
| title_short | Phylogenetic Patterns of Genus Megophrys (Anura: Megophryidae) from Java and Sumatra |
| title_sort | phylogenetic patterns of genus megophrys anura megophryidae from java and sumatra |
| topic | 16s rrna java megohprys sumatra zoogeography |
| url | https://jels.ub.ac.id/index.php/jels/article/view/235 |
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