Evaluation of Factors Affecting Uricase Production by the Screened Wild/Natural Microbes
Gout is a metabolic disorder due to the deposition of uric acid crystals within articular or periarticular tissues. Uricase (urate oxidase) catalyzes the oxidation of less water soluble uric acid (7 mg/dl) to a compound allantoin which is more water soluble(11g/L at 40°C) resulting into the ease of...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/976242 |
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| author | Harinath Dwivedi Kusum Agrawal Shubhini A Saraf |
| author_facet | Harinath Dwivedi Kusum Agrawal Shubhini A Saraf |
| author_sort | Harinath Dwivedi |
| collection | DOAJ |
| description | Gout is a metabolic disorder due to the deposition of uric acid crystals within articular or periarticular tissues. Uricase (urate oxidase) catalyzes the oxidation of less water soluble uric acid (7 mg/dl) to a compound allantoin which is more water soluble(11g/L at 40°C) resulting into the ease of excretion of uric acid. The objective of the work was to develop a new method for screening of microbes for uricase production and estimation of uricase thereof. This was achieved by utilizing the fact that uric acid dissolves on being acted upon by uricase. The proposed method is a novel, inexpensive, simple and sensitive technique for screening and estimation of uricase. Biomass and uricase production at different stages of microbial growth curve for the uricase producing microbe was studied. Effects of different medium components affecting uricase production by microbes were studied using Placket Burman statistical design. Addition of uric acid in the nutrient medium was found to be effective in increasing the uricase production by microbes growing in the medium. |
| format | Article |
| id | doaj-art-35cd2be3113a4c028343c31878834053 |
| institution | OA Journals |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-35cd2be3113a4c028343c318788340532025-08-20T02:24:07ZengWileyE-Journal of Chemistry0973-49452090-98102012-01-01942287229610.1155/2012/976242Evaluation of Factors Affecting Uricase Production by the Screened Wild/Natural MicrobesHarinath Dwivedi0Kusum Agrawal1Shubhini A Saraf2School of Pharmaceutics, Faculty of Pharmacy, Babu Banarasi Das University, Lucknow (Uttar Pradesh), IndiaSchool of Biotechnology, Shobhit University Meerut (Uttar Pradesh), IndiaBabasaheb Bhimrao Ambedkar University, Lucknow (Uttar Pradesh), IndiaGout is a metabolic disorder due to the deposition of uric acid crystals within articular or periarticular tissues. Uricase (urate oxidase) catalyzes the oxidation of less water soluble uric acid (7 mg/dl) to a compound allantoin which is more water soluble(11g/L at 40°C) resulting into the ease of excretion of uric acid. The objective of the work was to develop a new method for screening of microbes for uricase production and estimation of uricase thereof. This was achieved by utilizing the fact that uric acid dissolves on being acted upon by uricase. The proposed method is a novel, inexpensive, simple and sensitive technique for screening and estimation of uricase. Biomass and uricase production at different stages of microbial growth curve for the uricase producing microbe was studied. Effects of different medium components affecting uricase production by microbes were studied using Placket Burman statistical design. Addition of uric acid in the nutrient medium was found to be effective in increasing the uricase production by microbes growing in the medium.http://dx.doi.org/10.1155/2012/976242 |
| spellingShingle | Harinath Dwivedi Kusum Agrawal Shubhini A Saraf Evaluation of Factors Affecting Uricase Production by the Screened Wild/Natural Microbes E-Journal of Chemistry |
| title | Evaluation of Factors Affecting Uricase Production by the Screened Wild/Natural Microbes |
| title_full | Evaluation of Factors Affecting Uricase Production by the Screened Wild/Natural Microbes |
| title_fullStr | Evaluation of Factors Affecting Uricase Production by the Screened Wild/Natural Microbes |
| title_full_unstemmed | Evaluation of Factors Affecting Uricase Production by the Screened Wild/Natural Microbes |
| title_short | Evaluation of Factors Affecting Uricase Production by the Screened Wild/Natural Microbes |
| title_sort | evaluation of factors affecting uricase production by the screened wild natural microbes |
| url | http://dx.doi.org/10.1155/2012/976242 |
| work_keys_str_mv | AT harinathdwivedi evaluationoffactorsaffectinguricaseproductionbythescreenedwildnaturalmicrobes AT kusumagrawal evaluationoffactorsaffectinguricaseproductionbythescreenedwildnaturalmicrobes AT shubhiniasaraf evaluationoffactorsaffectinguricaseproductionbythescreenedwildnaturalmicrobes |