Annexin A3 Knockdown Suppresses Lung Adenocarcinoma
Our previous study identified an elevated abundance of annexin A3 (Anxa3) as a novel prognostic biomarker of lung adenocarcinoma (LADC) through quantitative proteomics analysis. However, the biological functions of Anxa3 in LADC are not fully clear. In this study, in vitro and in vivo assays were pe...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2016/4131403 |
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| author | Ying-Fu Liu Qing-Qing Liu Yue-Hua Zhang Jing-Hua Qiu |
| author_facet | Ying-Fu Liu Qing-Qing Liu Yue-Hua Zhang Jing-Hua Qiu |
| author_sort | Ying-Fu Liu |
| collection | DOAJ |
| description | Our previous study identified an elevated abundance of annexin A3 (Anxa3) as a novel prognostic biomarker of lung adenocarcinoma (LADC) through quantitative proteomics analysis. However, the biological functions of Anxa3 in LADC are not fully clear. In this study, in vitro and in vivo assays were performed to investigate the effects of Anxa3 downregulation on the growth, migration, invasion, metastasis, and signaling pathway activation of LADC cells. After Anxa3 downregulation, the growth of A549 and LTEP-a2 LADC cells was slowed and they showed decreased migration and invasion in vitro. Anxa3 knockdown significantly inhibited tumor formation by A549 cells in vivo; while many metastases were formed by control A549 cells, there were obvious reductions in the numbers of lung, liver, and brain metastases formed by Anxa3 knockdown in A549 cells. Furthermore, Anxa3 knockdown significantly decreased MMP-2 and N-cadherin expression and increased E-cadherin expression both in cell lines in vitro and in tumor nodules examined during in vivo tumorigenesis assays. Interestingly, Anxa3 downregulation reduced the phosphorylated levels of MEK and ERK. In summary, Anxa3 knockdown inhibited the growth, migration, invasion, and metastasis of LADC, decreased the activation of the MEK/ERK signaling pathway, and modulated the expression of MMP-2, E-cadherin, and N-cadherin. |
| format | Article |
| id | doaj-art-35c9309a783a49c8a563231a9a6b01a9 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-35c9309a783a49c8a563231a9a6b01a92025-08-20T03:55:16ZengWileyAnalytical Cellular Pathology2210-71772210-71852016-01-01201610.1155/2016/41314034131403Annexin A3 Knockdown Suppresses Lung AdenocarcinomaYing-Fu Liu0Qing-Qing Liu1Yue-Hua Zhang2Jing-Hua Qiu3Department of Basic Medical Sciences, Medical College, Xiamen University, Xiamen, Fujian, ChinaDepartment of Basic Medical Sciences, Medical College, Xiamen University, Xiamen, Fujian, ChinaLaboratory Animal Center, Xiamen University, Xiamen, Fujian, ChinaDepartment of Basic Medical Sciences, Medical College, Xiamen University, Xiamen, Fujian, ChinaOur previous study identified an elevated abundance of annexin A3 (Anxa3) as a novel prognostic biomarker of lung adenocarcinoma (LADC) through quantitative proteomics analysis. However, the biological functions of Anxa3 in LADC are not fully clear. In this study, in vitro and in vivo assays were performed to investigate the effects of Anxa3 downregulation on the growth, migration, invasion, metastasis, and signaling pathway activation of LADC cells. After Anxa3 downregulation, the growth of A549 and LTEP-a2 LADC cells was slowed and they showed decreased migration and invasion in vitro. Anxa3 knockdown significantly inhibited tumor formation by A549 cells in vivo; while many metastases were formed by control A549 cells, there were obvious reductions in the numbers of lung, liver, and brain metastases formed by Anxa3 knockdown in A549 cells. Furthermore, Anxa3 knockdown significantly decreased MMP-2 and N-cadherin expression and increased E-cadherin expression both in cell lines in vitro and in tumor nodules examined during in vivo tumorigenesis assays. Interestingly, Anxa3 downregulation reduced the phosphorylated levels of MEK and ERK. In summary, Anxa3 knockdown inhibited the growth, migration, invasion, and metastasis of LADC, decreased the activation of the MEK/ERK signaling pathway, and modulated the expression of MMP-2, E-cadherin, and N-cadherin.http://dx.doi.org/10.1155/2016/4131403 |
| spellingShingle | Ying-Fu Liu Qing-Qing Liu Yue-Hua Zhang Jing-Hua Qiu Annexin A3 Knockdown Suppresses Lung Adenocarcinoma Analytical Cellular Pathology |
| title | Annexin A3 Knockdown Suppresses Lung Adenocarcinoma |
| title_full | Annexin A3 Knockdown Suppresses Lung Adenocarcinoma |
| title_fullStr | Annexin A3 Knockdown Suppresses Lung Adenocarcinoma |
| title_full_unstemmed | Annexin A3 Knockdown Suppresses Lung Adenocarcinoma |
| title_short | Annexin A3 Knockdown Suppresses Lung Adenocarcinoma |
| title_sort | annexin a3 knockdown suppresses lung adenocarcinoma |
| url | http://dx.doi.org/10.1155/2016/4131403 |
| work_keys_str_mv | AT yingfuliu annexina3knockdownsuppresseslungadenocarcinoma AT qingqingliu annexina3knockdownsuppresseslungadenocarcinoma AT yuehuazhang annexina3knockdownsuppresseslungadenocarcinoma AT jinghuaqiu annexina3knockdownsuppresseslungadenocarcinoma |