Critical parameters for robust Agrobacterium‐mediated transient transformation and quantitative promoter assays in Catharanthus roseus seedlings
Abstract Agrobacterium‐mediated transient expression methods are widely used to study gene function in both model and non‐model plants. Using a dual‐luciferase assay, we quantified the effect of Agrobacterium‐infiltration parameters on the transient transformation efficiency of Catharanthus roseus s...
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Main Authors: | Lauren F. Cole‐Osborn, Emma Meehan, Carolyn W. T. Lee‐Parsons |
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Format: | Article |
Language: | English |
Published: |
Wiley
2024-06-01
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Series: | Plant Direct |
Subjects: | |
Online Access: | https://doi.org/10.1002/pld3.596 |
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