<i>Rhizobium</i> sp. as a Growth Inducer of <i>Phaseolus vulgaris</i> L., Determining the Qualitative Chemical Composition of Its Ethyl Acetate Extract Using High-Resolution Liquid Chromatography Coupled with Mass Spectrometry
<i>Phaseolus vulgaris</i> L. is one of the most important legumes for human consumption due to its contents of proteins, antioxidants, minerals, and bioactive compounds. In the last decade, there has been a growing research interest in increasing yields while reducing or replacing the us...
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2025-03-01
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| Series: | International Journal of Plant Biology |
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| author | Giselle Hernández Yoania Ríos Trina H. García Yusset Louis Iraida Spengler Yarelis Ortiz |
| author_facet | Giselle Hernández Yoania Ríos Trina H. García Yusset Louis Iraida Spengler Yarelis Ortiz |
| author_sort | Giselle Hernández |
| collection | DOAJ |
| description | <i>Phaseolus vulgaris</i> L. is one of the most important legumes for human consumption due to its contents of proteins, antioxidants, minerals, and bioactive compounds. In the last decade, there has been a growing research interest in increasing yields while reducing or replacing the use of chemical fertilizers. This has led to a focus on plant growth-promoting Rhizobacteria (PGPR) as biofertilizers in sustainable agricultural practices. The aim of this study was to determine the growth-promoting activity of a culture broth of the Gram-negative soil bacteria <i>Rhizobium</i> sp. (F7), which is conserved in the Collection of Beneficial Bacteria at the Institute of Fundamental Research in Tropical Agriculture (INIFAT), and to identify the main secondary metabolites present in the ethyl acetate crude extract using high-resolution liquid chromatography coupled with mass spectrometry (UHPLC-ESI-MS/MS). The growth-promoting activity of the culture broth on <i>Phaseolus vulgaris</i> L. seeds was evaluated. The ethyl acetate extract was obtained by liquid–liquid extraction with ethyl acetate from the culture broth, and UHPLC-ESI-MS/MS was used to identify secondary metabolites. The results indicated that the culture broth of <i>Rhizobium</i> sp. exhibited an in vitro growth-stimulating effect. Furthermore, ten secondary metabolites were identified in the ethyl acetate extract (p-coumaric acid, indole-3-lactic acid, naringenin, and siderophores B and C, among others). These findings highlight the bioactive metabolites produced by <i>Rhizobium</i> sp., a bacterial strain of the INIFAT collection, which have a positive effect as growth promoters in plants. They reveal the potential of <i>Rhizobium</i> sp. as a promising candidate for inclusion in agricultural management practices. |
| format | Article |
| id | doaj-art-35a28fbc65da4719a0234c19530e0df8 |
| institution | DOAJ |
| issn | 2037-0164 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | International Journal of Plant Biology |
| spelling | doaj-art-35a28fbc65da4719a0234c19530e0df82025-08-20T02:42:34ZengMDPI AGInternational Journal of Plant Biology2037-01642025-03-011613710.3390/ijpb16010037<i>Rhizobium</i> sp. as a Growth Inducer of <i>Phaseolus vulgaris</i> L., Determining the Qualitative Chemical Composition of Its Ethyl Acetate Extract Using High-Resolution Liquid Chromatography Coupled with Mass SpectrometryGiselle Hernández0Yoania Ríos1Trina H. García2Yusset Louis3Iraida Spengler4Yarelis Ortiz5Center for Natural Products Research, Faculty of Chemistry, University of Havana, Havana 10400, CubaInstitute of Fundamental Research in Tropical Agriculture “Alexander von Humboldt” (INIFAT), 188 St # 38754 Between 397 and Linderos, Santiago de Las Vegas, Havana 17200, CubaCenter for Natural Products Research, Faculty of Chemistry, University of Havana, Havana 10400, CubaCenter for Natural Products Research, Faculty of Chemistry, University of Havana, Havana 10400, CubaCenter for Natural Products Research, Faculty of Chemistry, University of Havana, Havana 10400, CubaInstitute of Fundamental Research in Tropical Agriculture “Alexander von Humboldt” (INIFAT), 188 St # 38754 Between 397 and Linderos, Santiago de Las Vegas, Havana 17200, Cuba<i>Phaseolus vulgaris</i> L. is one of the most important legumes for human consumption due to its contents of proteins, antioxidants, minerals, and bioactive compounds. In the last decade, there has been a growing research interest in increasing yields while reducing or replacing the use of chemical fertilizers. This has led to a focus on plant growth-promoting Rhizobacteria (PGPR) as biofertilizers in sustainable agricultural practices. The aim of this study was to determine the growth-promoting activity of a culture broth of the Gram-negative soil bacteria <i>Rhizobium</i> sp. (F7), which is conserved in the Collection of Beneficial Bacteria at the Institute of Fundamental Research in Tropical Agriculture (INIFAT), and to identify the main secondary metabolites present in the ethyl acetate crude extract using high-resolution liquid chromatography coupled with mass spectrometry (UHPLC-ESI-MS/MS). The growth-promoting activity of the culture broth on <i>Phaseolus vulgaris</i> L. seeds was evaluated. The ethyl acetate extract was obtained by liquid–liquid extraction with ethyl acetate from the culture broth, and UHPLC-ESI-MS/MS was used to identify secondary metabolites. The results indicated that the culture broth of <i>Rhizobium</i> sp. exhibited an in vitro growth-stimulating effect. Furthermore, ten secondary metabolites were identified in the ethyl acetate extract (p-coumaric acid, indole-3-lactic acid, naringenin, and siderophores B and C, among others). These findings highlight the bioactive metabolites produced by <i>Rhizobium</i> sp., a bacterial strain of the INIFAT collection, which have a positive effect as growth promoters in plants. They reveal the potential of <i>Rhizobium</i> sp. as a promising candidate for inclusion in agricultural management practices.https://www.mdpi.com/2037-0164/16/1/37rhizobacteriametabolites<i>Phaseolus vulgaris</i>grainUHPLC-ESI-MS/MS |
| spellingShingle | Giselle Hernández Yoania Ríos Trina H. García Yusset Louis Iraida Spengler Yarelis Ortiz <i>Rhizobium</i> sp. as a Growth Inducer of <i>Phaseolus vulgaris</i> L., Determining the Qualitative Chemical Composition of Its Ethyl Acetate Extract Using High-Resolution Liquid Chromatography Coupled with Mass Spectrometry International Journal of Plant Biology rhizobacteria metabolites <i>Phaseolus vulgaris</i> grain UHPLC-ESI-MS/MS |
| title | <i>Rhizobium</i> sp. as a Growth Inducer of <i>Phaseolus vulgaris</i> L., Determining the Qualitative Chemical Composition of Its Ethyl Acetate Extract Using High-Resolution Liquid Chromatography Coupled with Mass Spectrometry |
| title_full | <i>Rhizobium</i> sp. as a Growth Inducer of <i>Phaseolus vulgaris</i> L., Determining the Qualitative Chemical Composition of Its Ethyl Acetate Extract Using High-Resolution Liquid Chromatography Coupled with Mass Spectrometry |
| title_fullStr | <i>Rhizobium</i> sp. as a Growth Inducer of <i>Phaseolus vulgaris</i> L., Determining the Qualitative Chemical Composition of Its Ethyl Acetate Extract Using High-Resolution Liquid Chromatography Coupled with Mass Spectrometry |
| title_full_unstemmed | <i>Rhizobium</i> sp. as a Growth Inducer of <i>Phaseolus vulgaris</i> L., Determining the Qualitative Chemical Composition of Its Ethyl Acetate Extract Using High-Resolution Liquid Chromatography Coupled with Mass Spectrometry |
| title_short | <i>Rhizobium</i> sp. as a Growth Inducer of <i>Phaseolus vulgaris</i> L., Determining the Qualitative Chemical Composition of Its Ethyl Acetate Extract Using High-Resolution Liquid Chromatography Coupled with Mass Spectrometry |
| title_sort | i rhizobium i sp as a growth inducer of i phaseolus vulgaris i l determining the qualitative chemical composition of its ethyl acetate extract using high resolution liquid chromatography coupled with mass spectrometry |
| topic | rhizobacteria metabolites <i>Phaseolus vulgaris</i> grain UHPLC-ESI-MS/MS |
| url | https://www.mdpi.com/2037-0164/16/1/37 |
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